{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T16:33:45Z","timestamp":1761323625202,"version":"3.32.0"},"reference-count":33,"publisher":"Maximum Academic Press","issue":"2","license":[{"start":{"date-parts":[[2009,6,2]],"date-time":"2009-06-02T00:00:00Z","timestamp":1243900800000},"content-version":"unspecified","delay-in-days":4841,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Vis Neurosci"],"published-print":{"date-parts":[[1996,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Two distinct opsin-encoding cDNAs, designated MANOP1 and MANOP2, were isolated as 3\u2032 fragments from the sphingid moth<jats:italic>Manduca sexta<\/jats:italic>. They were obtained by reverse transcription of retinal RNA and amplification with the polymerase chain reaction (PCR) using a degenerate primer designed to an amino-acid sequence conserved in arthropod opsins. The cDNA fragments labelled bands at approximately 1.8 kb on Northern blots of retinal RNA extracts. Levels of opsin message were compared in retinas from normal moths, whose diets were fortified with carotenoid precursors of the<jats:italic>Manduca<\/jats:italic>rhodopsin chromophore, 3-hydroxyretinal, and those reared on carotenoid\/retinoid (vitamin A) deficient diets. The chromophore-depleted retinas contained more opsin mRNA; this was particularly true for MANOP2. Thus, the chromophore is not required for opsin gene transcription in<jats:italic>Manduca<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s0952523800007598","type":"journal-article","created":{"date-parts":[[2009,6,2]],"date-time":"2009-06-02T11:25:13Z","timestamp":1243941913000},"page":"353-358","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":6,"title":["Expression of opsin mRNA in normal and vitamin A deficient retinas of the sphingid moth<i>Manduca sexta<\/i>"],"prefix":"10.48130","volume":"13","author":[{"given":"Michael R.","family":"Chase","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruth R.","family":"Bennett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard H.","family":"White","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"27968","published-online":{"date-parts":[[2009,6,2]]},"reference":[{"key":"S0952523800007598_ref028","doi-asserted-by":"publisher","DOI":"10.1007\/BF00221461"},{"key":"S0952523800007598_ref008","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1002\/j.1460-2075.1987.tb04774.x","article-title":"An opsin gene that is expressed only in the R7 photoreceptor cell of Drosophila","volume":"6","author":"Fryxell","year":"1987","journal-title":"EMBO Journal"},{"key":"S0952523800007598_ref004","doi-asserted-by":"publisher","DOI":"10.1017\/S0952523800001322"},{"key":"S0952523800007598_ref025","doi-asserted-by":"publisher","DOI":"10.1016\/1043-2760(94)90076-0"},{"key":"S0952523800007598_ref017","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1983.tb07869.x"},{"key":"S0952523800007598_ref016","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(93)90059-Z"},{"key":"S0952523800007598_ref003","doi-asserted-by":"publisher","DOI":"10.1007\/BF00612992"},{"key":"S0952523800007598_ref027","first-page":"640","article-title":"Manduca opsin mRNA in relation to retinoids and light\/dark cycle","volume":"17","author":"White","year":"1991","journal-title":"Society for Neuroscience Abstracts"},{"key":"S0952523800007598_ref024","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1993.1113"},{"key":"S0952523800007598_ref005","doi-asserted-by":"publisher","DOI":"10.1006\/mpev.1995.1004"},{"key":"S0952523800007598_ref023","first-page":"S771","article-title":"Transcriptional control of Drosophila opsins by retinoids","volume":"36","author":"Stark","year":"1995","journal-title":"Investigative Ophthalmology and Visual Science"},{"key":"S0952523800007598_ref012","first-page":"219","volume-title":"Methods in Enzymology","volume":"152","author":"Macdonald","year":"1987"},{"key":"S0952523800007598_ref007","first-page":"50","article-title":"Monoclonal antibodies provide evidence that rhodopsin in the outer rhabdomeres of Drosophila melanogaster is not glycosylated","volume":"44","author":"Decouet","year":"1987","journal-title":"European Journal of Cell Biology"},{"key":"S0952523800007598_ref011","first-page":"1968","article-title":"Maintenance of opsin density in photoreceptor outer segments of retinoid-deprived rats","volume":"32","author":"Katz","year":"1991","journal-title":"Investigative Ophthalmology and Visual Science"},{"key":"S0952523800007598_ref010","first-page":"50","article-title":"Opsin maturation and targeting to rhabdomeral photoreceptor membranes requires the retinal chromophore","volume":"44","author":"Huber","year":"1994","journal-title":"European Journal of Cell Biology"},{"key":"S0952523800007598_ref002","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6989(85)90002-1"},{"key":"S0952523800007598_ref006","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90836-6"},{"key":"S0952523800007598_ref009","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(93)81180-8"},{"key":"S0952523800007598_ref013","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.07-05-01558.1987"},{"key":"S0952523800007598_ref014","doi-asserted-by":"crossref","DOI":"10.1093\/oso\/9780198547709.001.0001","volume-title":"Retinoids in Normal Development and Teratogenesis","author":"Morriss-Kay","year":"1992"},{"key":"S0952523800007598_ref015","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90343-5"},{"volume-title":"Molecular Cloning a Laboratory Manual","year":"1989","author":"Sambrook","key":"S0952523800007598_ref018"},{"key":"S0952523800007598_ref019","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(91)90147-7"},{"key":"S0952523800007598_ref020","first-page":"277","volume-title":"Structure and Functions of Retinal Proteins","author":"Schwemer","year":"1992"},{"key":"S0952523800007598_ref021","doi-asserted-by":"publisher","DOI":"10.1016\/0965-1748(92)90119-Y"},{"key":"S0952523800007598_ref022","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.13.6150"},{"key":"S0952523800007598_ref026","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(94)90101-5"},{"key":"S0952523800007598_ref029","doi-asserted-by":"publisher","DOI":"10.1007\/BF00381880"},{"key":"S0952523800007598_ref030","first-page":"776","article-title":"Opsin genes from Manduca and their expression in vitamin A deficiency","volume":"20","author":"White","year":"1994","journal-title":"Society for Neuroscience Abstracts"},{"key":"S0952523800007598_ref031","doi-asserted-by":"publisher","DOI":"10.1007\/BF00606365"},{"key":"S0952523800007598_ref032","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90344-7"},{"key":"S0952523800007598_ref033","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.07-05-01550.1987"},{"key":"S0952523800007598_ref001","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1002\/j.1460-2075.1993.tb05814.x","article-title":"The probable arrangement of the helices in G protein-coupled receptors","volume":"12","author":"Balwdin","year":"1993","journal-title":"EMBO Journal"}],"container-title":["Visual Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0952523800007598","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,2]],"date-time":"2025-01-02T19:57:44Z","timestamp":1735847864000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0952523800007598\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,3]]},"references-count":33,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1996,3]]}},"alternative-id":["S0952523800007598"],"URL":"https:\/\/doi.org\/10.1017\/s0952523800007598","relation":{},"ISSN":["0952-5238","1469-8714"],"issn-type":[{"type":"print","value":"0952-5238"},{"type":"electronic","value":"1469-8714"}],"subject":[],"published":{"date-parts":[[1996,3]]},"assertion":[{"value":"Cambridge University Press 1996","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}