{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,1,3]],"date-time":"2025-01-03T05:40:40Z","timestamp":1735882840660,"version":"3.32.0"},"reference-count":0,"publisher":"Maximum Academic Press","issue":"6","license":[{"start":{"date-parts":[[2001,4,9]],"date-time":"2001-04-09T00:00:00Z","timestamp":986774400000},"content-version":"unspecified","delay-in-days":159,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vis Neurosci"],"published-print":{"date-parts":[[2000,11]]},"abstract":"<jats:p>In retinal rods, light exposure decreases the total outer \nsegment content of both cGMP and cAMP by about 50%. The \nfunctional role of the light-evoked change in cAMP is not \nknown. It is postulated to trigger changes in the phosphorylation \nstate of phosducin, a phosphoprotein that is phosphorylated \nin the dark by cAMP-dependent protein kinase (PKA) and \ndephosphorylated by basal phosphatase activity when PKA \nis inhibited by the light-evoked drop in cAMP. In biochemical \nstudies, dephosphorylated phosducin binds to free \u03b2\u03b3 \ndimer of transducin (T\u03b2\u03b3) and prevents the regeneration \nof heterotrimeric transducin by blocking the re-association \nof the \u03b2\u03b3 and \u03b1 subunits. Phosducin's interaction \nwith T\u03b2\u03b3 is blocked when it is phosphorylated on a single \nresidue by PKA. To evaluate the effect of the light-evoked fall \nin cAMP, functionally intact isolated lizard rod outer \nsegments were dialyzed in whole-cell voltage clamp with \na standard internal solution and electrical light responses \nwere recorded with and without adding cAMP to the dialysis \nsolution. Since the total outer segment content of cAMP \nin darkness is \u223c5 \u03bcM, internal dialysis with solution \ncontaining a much higher concentration (100 \u03bcM) of \ncAMP (or 8-bromo-cAMP) will overcome the effects of a light-evoked \ndecrease in its concentration by keeping cAMP-dependent \nprocesses fully activated. Neither cyclic nucleotide had \nany influence on the generation, light sensitivity, recovery, \nor background adaptation of the flash response. These results \nalso argue against the participation of phosducin in the \nsequence of events that are responsible for these aspects \nof rod function. This does not exclude the possibility \nof phosducin being involved in adaptation caused by higher \nlight levels than used in the present study, that is, bleaching \nadaptation, or in light-dependent processes other than \nphototransduction.<\/jats:p>","DOI":"10.1017\/s0952523800176072","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:39:08Z","timestamp":1027777148000},"page":"887-892","source":"Crossref","is-referenced-by-count":7,"title":["Cyclic AMP has no effect on the generation, recovery, or background \nadaptation of light responses in functionally intact rod outer segments: \nWith implications about the function of phosducin"],"prefix":"10.48130","volume":"17","author":[{"given":"HANA","family":"JINDROVA","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PETER B.","family":"DETWILER","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"27968","published-online":{"date-parts":[[2001,4,9]]},"container-title":["Visual Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0952523800176072","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,2]],"date-time":"2025-01-02T20:00:37Z","timestamp":1735848037000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0952523800176072\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,11]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2000,11]]}},"alternative-id":["S0952523800176072"],"URL":"https:\/\/doi.org\/10.1017\/s0952523800176072","relation":{},"ISSN":["0952-5238","1469-8714"],"issn-type":[{"type":"print","value":"0952-5238"},{"type":"electronic","value":"1469-8714"}],"subject":[],"published":{"date-parts":[[2000,11]]}}}