{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T13:47:45Z","timestamp":1761918465146,"version":"3.32.0"},"reference-count":0,"publisher":"Maximum Academic Press","issue":"5","license":[{"start":{"date-parts":[[2002,5,20]],"date-time":"2002-05-20T00:00:00Z","timestamp":1021852800000},"content-version":"unspecified","delay-in-days":261,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Vis Neurosci"],"published-print":{"date-parts":[[2001,9]]},"abstract":"<jats:p>Glutamate and kainate-induced currents of primate ganglion \ncells were studied using the whole-cell patch-clamp technique \nin a retinal slice preparation. Antagonists and allosteric \nmodulators of desensitization selective for either \n\u03b1-amino-3-hydroxy-5-methyl-4-isoazoleprionic acid (AMPA)- \nor kainate-preferring receptors were used to determine the \ncontributions of each type of receptor to excitatory responses. \nWith synaptic transmission and NMDA receptors blocked, the \nAMPA-preferring receptor antagonist GYKI 52466 (30 \u03bcM\u2013100 \n\u03bcM) reversibly blocked most of the glutamate-induced current \nin ganglion cells. GYKI 52466 also blocked the response in ganglion \ncells to focally applied kainate, suggesting that the current \nresponse to kainate arises from activation of AMPA-preferring \nreceptors, and not kainate-preferring receptors. Both cyclothiazide \n(10 \u03bcM\u2013100 \u03bcM) and the novel drug \n4-[2-(phenylsulfonylamino)ethylthio]-2,6-difluoro-phenoxyacetamide\n (PEPA, 20 \u03bcM\u2013100 \u03bcM), which selectively enhance \nresponses mediated by AMPA-preferring receptors, enhanced \nglutamate-induced responses of ganglion cells. Since these drugs \npreferentially inhibit desensitization of the flip and flop \nsplice variants, respectively, of AMPA-preferring receptors, \nit is likely that both splice variants are present on these \nganglion cells. Concanavalin A, which selectively suppresses \nthe desensitization of kainate-preferring receptors, had no \neffect on the glutamate-induced responses of ganglion cells. \nWe conclude that the non-NMDA component of the excitatory, \nglutamatergic input to primate ganglion cells is mediated largely \nby AMPA-preferring receptors, with little, if any, \nkainate-preferring receptor-mediated response.<\/jats:p>","DOI":"10.1017\/s0952523801185044","type":"journal-article","created":{"date-parts":[[2002,12,11]],"date-time":"2002-12-11T22:09:45Z","timestamp":1039644585000},"page":"703-710","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":16,"title":["AMPA-preferring receptors mediate excitatory non-NMDA responses \nof primate retinal ganglion cells"],"prefix":"10.48130","volume":"18","author":[{"given":"ROY A.","family":"JACOBY","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SAMUEL M.","family":"WU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"27968","published-online":{"date-parts":[[2002,5,20]]},"container-title":["Visual Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0952523801185044","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,2]],"date-time":"2025-01-02T20:00:56Z","timestamp":1735848056000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0952523801185044\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,9]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2001,9]]}},"alternative-id":["S0952523801185044"],"URL":"https:\/\/doi.org\/10.1017\/s0952523801185044","relation":{},"ISSN":["0952-5238","1469-8714"],"issn-type":[{"type":"print","value":"0952-5238"},{"type":"electronic","value":"1469-8714"}],"subject":[],"published":{"date-parts":[[2001,9]]},"assertion":[{"name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}