{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T22:21:11Z","timestamp":1770070871479,"version":"3.49.0"},"reference-count":0,"publisher":"Maximum Academic Press","issue":"2","license":[{"start":{"date-parts":[[2002,7,2]],"date-time":"2002-07-02T00:00:00Z","timestamp":1025568000000},"content-version":"unspecified","delay-in-days":123,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Vis Neurosci"],"published-print":{"date-parts":[[2002,3]]},"abstract":"<jats:p>Alpha-2 adrenoceptor agonists have previously been shown to \nenhance neuronal survival in an optic nerve mechanical injury \nmodel and to protect photoreceptors in a light-induced degeneration \nmodel. The purpose of this study was to examine the effect of \nthe alpha-2 adrenoceptor agonist in a pressure-induced retinal \nischemia model. Brown-Norway rats were treated systemically \nor topically with alpha-2 adrenoceptor specific agonist \nbrimonidine. Retinal ischemia was induced by increasing the \nintraocular pressure to 110 mm Hg for 50 min. The effect of \nbrimonidine on retinal ischemic injury was functionally assessed \nin the rats 7 d later using electroretinography (ERG). \nIschemia-induced retinal cell death was studied using the terminal \ndeoxynucleotidyl transferase-mediated dUTP nick end-labeling \n(TUNEL) staining. We found that brimonidine treatment significantly \nprotected the retina from retinal ischemic injury in a dose- \nand time-dependent manner. This protection can be achieved either \nby systemic or topical application and can be blocked by \npretreatment with the alpha-2 adrenoceptor antagonist, yohimbine. \nUsing reverse transcription-polymerase chain reaction (RT-PCR) \nand Western blot analysis, we found that brimonidine can \nup-regulate the expression of basic fibroblast growth factor, \nbcl-2 and bcl-xl in the retina. The drug also can activate two \nmajor cell survival signaling pathways in the retina: the \nextracellular-signal-regulated kinases (ERKs) and \nphosphatidylinositol-3\u2032 kinase\/protein kinase Akt pathways. \nAll these aforementioned factors may potentially contribute \nin mediating brimonidine's protective effect in this acute \nretinal ischemia model.<\/jats:p>","DOI":"10.1017\/s0952523802191152","type":"journal-article","created":{"date-parts":[[2002,12,11]],"date-time":"2002-12-11T22:10:34Z","timestamp":1039644634000},"page":"175-185","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":65,"title":["Alpha-2 adrenoceptor agonist protects retinal function after \nacute retinal ischemic injury in the rat"],"prefix":"10.48130","volume":"19","author":[{"given":"RONALD K.","family":"LAI","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"TERESA","family":"CHUN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DAIN","family":"HASSON","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"STEVE","family":"LEE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"FARROKH","family":"MEHRBOD","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LARRY","family":"WHEELER","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"27968","published-online":{"date-parts":[[2002,7,2]]},"container-title":["Visual Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0952523802191152","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,2]],"date-time":"2025-01-02T20:01:08Z","timestamp":1735848068000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0952523802191152\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,3]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2002,3]]}},"alternative-id":["S0952523802191152"],"URL":"https:\/\/doi.org\/10.1017\/s0952523802191152","relation":{},"ISSN":["0952-5238","1469-8714"],"issn-type":[{"value":"0952-5238","type":"print"},{"value":"1469-8714","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,3]]},"assertion":[{"value":"2002 Cambridge University Press","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}