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The<jats:italic>Thy1-<\/jats:italic>CFP D2 (CFP-D2) line was created by congenically breeding the D2 line, which develops pigmentary glaucoma, and the<jats:italic>Thy1<\/jats:italic>-CFP line, which expresses CFP in ganglion cells. Microsatellite marker analysis of CFP-D2 progeny verified the genetic inclusion of the D2<jats:italic>isa<\/jats:italic>and<jats:italic>ipd<\/jats:italic>loci. Specific mutations within these loci lead to dysfunctional melanosomal proteins and glaucomatous phenotype in D2 mice. Polymerase chain reaction analysis confirmed the inclusion of the<jats:italic>Thy1<\/jats:italic>-CFP transgene. CFP-fluorescent ganglion cells, 6\u201320<jats:italic>\u03bc<\/jats:italic>m in diameter, were distributed in all retinal regions, CFP processes were throughout the inner plexiform layer, and CFP-fluorescent axons were in the fiber layer and optic nerve head. Immunohistochemistry with antibodies to ganglion cell markers NF-L, NeuN, Brn3a, and SMI32 was used to confirm CFP expression in ganglion cells. Immunohistochemistry with antibodies to amacrine cell markers HPC-1 and ChAT was used to confirm weak CFP expression in cholinergic amacrine cells. CFP-D2 mice developed a glaucomatous phenotype, including iris disease, ganglion cell loss, attrition of the fiber layer, and elevated intraocular pressure. A CFP-D2 transgenic line with CFP-expressing ganglion cells was developed, which has (1) a predominantly D2 genetic background, (2) CFP-expressing ganglion cells, and (3) age-related progressive glaucoma. This line will be of value for experimental studies investigating ganglion cells and their axons<jats:italic>in vivo<\/jats:italic>and<jats:italic>in vitro<\/jats:italic>during the progressive development of glaucoma.<\/jats:p>","DOI":"10.1017\/s095252380999023x","type":"journal-article","created":{"date-parts":[[2009,11,23]],"date-time":"2009-11-23T11:48:59Z","timestamp":1258976939000},"page":"453-465","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":22,"title":["A<i>Thy1<\/i>-CFP DBA\/2J mouse line with cyan fluorescent protein expression in retinal ganglion cells"],"prefix":"10.48130","volume":"26","author":[{"given":"IONA D.","family":"RAYMOND","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ANGELA 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