{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T00:37:44Z","timestamp":1770511064039,"version":"3.49.0"},"reference-count":31,"publisher":"Maximum Academic Press","issue":"1","license":[{"start":{"date-parts":[[2013,12,23]],"date-time":"2013-12-23T00:00:00Z","timestamp":1387756800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Vis Neurosci"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The <jats:italic>\u03b1<\/jats:italic><jats:sub>2<\/jats:sub><jats:italic>\u03b4<\/jats:italic> auxiliary subunits of voltage-gated Ca<jats:sup>2+<\/jats:sup> channels (VGCCs) are important modulators of VGCC function. Gabapentin interacts with <jats:italic>\u03b1<\/jats:italic><jats:sub>2<\/jats:sub><jats:italic>\u03b4<\/jats:italic><jats:sub>1<\/jats:sub> and <jats:italic>\u03b1<\/jats:italic><jats:sub>2<\/jats:sub><jats:italic>\u03b4<\/jats:italic><jats:sub>2<\/jats:sub> subunits and is reported to reduce Ca<jats:sup>2+<\/jats:sup> channel current amplitude (<jats:italic>I<\/jats:italic><jats:sub>Ca<\/jats:sub>). This study aimed to determine the effects of gabapentin on VGCCs in retinal ganglion cells (RGCs). Whole cell patch clamp was used to record <jats:italic>I<\/jats:italic><jats:sub>Ca<\/jats:sub> in isolated RGCs, and calcium imaging was used to measure Ca<jats:sup>2+<\/jats:sup> transients from RGCs <jats:italic>in situ<\/jats:italic>. Immunohistochemistry was used to detect the presence of <jats:italic>\u03b1<\/jats:italic><jats:sub>2<\/jats:sub><jats:italic>\u03b4<\/jats:italic><jats:sub>1<\/jats:sub>-containing VGCCs in isolated RGCs in the absence and presence of gabapentin pretreatment. Acute administration of gabapentin reduced <jats:italic>I<\/jats:italic><jats:sub>Ca<\/jats:sub> and Ca<jats:sup>2+<\/jats:sup> transients compared to control conditions. In isolated RGCs, pretreatment with gabapentin (4\u201318 h) reduced <jats:italic>I<\/jats:italic><jats:sub>Ca<\/jats:sub>, and cell surface <jats:italic>\u03b1<\/jats:italic><jats:sub>2<\/jats:sub><jats:italic>\u03b4<\/jats:italic><jats:sub>1<\/jats:sub> staining was reduced compared to nonpretreated cells. Acute administration of gabapentin to isolated RGCs that had been pretreated further reduced <jats:italic>I<\/jats:italic><jats:sub>Ca<\/jats:sub>. These results show that gabapentin has both short-term and long-term mechanisms to reduce <jats:italic>I<\/jats:italic><jats:sub>Ca<\/jats:sub> in isolated RGCs. Some Ca<jats:sup>2+<\/jats:sup> channel blockers have been shown to protect RGCs in retinal trauma suggesting that modulation of VGCCs by gabapentin may prevent the deleterious effects of elevated Ca<jats:sup>2+<\/jats:sup> levels in RGCs in trauma and disease.<\/jats:p>","DOI":"10.1017\/s0952523813000588","type":"journal-article","created":{"date-parts":[[2013,12,23]],"date-time":"2013-12-23T11:24:44Z","timestamp":1387797884000},"page":"47-55","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":8,"title":["Modulation of voltage-gated Ca<sup>2+<\/sup> channels in rat retinal ganglion cells by gabapentin"],"prefix":"10.48130","volume":"31","author":[{"given":"SPRING R.","family":"FARRELL","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ALLISON","family":"SARGOY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"NICHOLAS 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