{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T05:49:45Z","timestamp":1767851385522,"version":"3.49.0"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2001,5,17]],"date-time":"2001-05-17T00:00:00Z","timestamp":990057600000},"content-version":"unspecified","delay-in-days":105,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2001,2]]},"abstract":"<jats:p>In this paper the effects of capacitation and fertilisation stimulating\ncompounds (heparin, caffeine, glucose, D-penicillamine, bovine serum (BOS),\nbovine serum albumin (BSA), polyvinyl alcohol (PVA)) were analysed in\nseveral <jats:italic>in vitro<\/jats:italic> fertilisation protocols. Attention was paid to the rate\nof penetrated oocytes, kinetics of penetration and to polyspermic\nfertilisation. Cryopreserved bovine sperm and <jats:italic>in vitro<\/jats:italic> matured bovine\noocytes were used throughout all the fertilisation experiments. As detected\nin the first 8 h fertilisation experiment with non-incubated sperm, the\nsupplementation of medium with heparin, BOS and glucose supported the\nfertilisation rate most effectively (100%), including the kinetics of\npronuclei formation (52.4%). The absence of BOS resulted in a decreased\nfertilisation rate (62.7%) as well as a delay in pronuclei formation\n(13.6%), similar to that after substitution of heparin with caffeine\n(73.0% and 25.4%, respectively). The penetration rate in the control\nmedium with BOS (without heparin and caffeine) was surprisingly\nhigh, especially in medium without glucose (62.2%). The positive effect of\nglucose on sperm penetration was observed mainly in a chemically defined\nmedium with PVA. High polyspermy rates were observed throughout all\nexperiments in the media containing heparin or caffeine and BOS as the\nmacromolecular component. D-Penicillamine was not shown to be a\nfertilisation-stimulating molecule. However, as detected in the second\nexperiment in which oocytes were fertilised with 5 h incubated sperm,\nits positive effect on the prolongation of a fertile life span of\ncryopreserved spermatozoa was significant. The presence of either\ncaffeine or heparin in the fertilisation medium (FM) with BOS during\nsperm incubation induced tyrosine phosphorylation of an approximately\n90 kDa protein, detected after 5 h of sperm incubation. The absence of\nBOS reduced tyrosine phosphorylation of this protein in fertilisation\nmedium with heparin. The percentage of motile spermatozoa and those with\nintact acrosomes were monitored throughout all experiments.<\/jats:p>","DOI":"10.1017\/s0967199401001046","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:17:11Z","timestamp":1027761431000},"page":"25-38","source":"Crossref","is-referenced-by-count":11,"title":["The effect of various capacitation active compounds and capacitation time on the <i>in vitro<\/i> fertility and protein tyrosine phosphorylation profiles of bovine sperm"],"prefix":"10.1017","volume":"9","author":[{"given":"A.","family":"Pavlok","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Kubelka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"P\u011bknicoc\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2001,5,17]]},"container-title":["Zygote"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0967199401001046","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,8]],"date-time":"2019-05-08T15:02:57Z","timestamp":1557327777000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0967199401001046\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,2]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2001,2]]}},"alternative-id":["S0967199401001046"],"URL":"https:\/\/doi.org\/10.1017\/s0967199401001046","relation":{},"ISSN":["0967-1994","1469-8730"],"issn-type":[{"value":"0967-1994","type":"print"},{"value":"1469-8730","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,2]]}}}