{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T23:42:49Z","timestamp":1769298169892,"version":"3.49.0"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2006,3,24]],"date-time":"2006-03-24T00:00:00Z","timestamp":1143158400000},"content-version":"unspecified","delay-in-days":51,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:p>The aim of the work was to study a potential relationship between acrosome response characteristics of bovine spermatozoa and their ability to fertilize oocytes and produce <jats:italic>in vitro<\/jats:italic> embryos. Sperm of artificial insemination bulls with a high rate (22.0 \u00b1 4.1%, group A, <jats:italic>n<\/jats:italic> = 7) or a low rate (10.3 \u00b1 4.1%, group B, <jats:italic>n<\/jats:italic> = 8) of embryos were used. For acrosome assessment, motile spermatozoa from a Percoll gradient were incubated with or without heparin and examined by the fix-vital sperm assay (FVSA). The differences between the heparin-treated (H<jats:sup>+<\/jats:sup>) and the non-treated (H<jats:sup>\u2212<\/jats:sup>) spermatozoa were significant (<jats:italic>p<\/jats:italic> &lt; 0.01) in all bulls at all tested intervals. According to the kinetics of the heparin response, the bulls fell into three categories: fast (FR, <jats:italic>n<\/jats:italic> = 7), moderate (MR, <jats:italic>n<\/jats:italic> = 5) or slow (SR, <jats:italic>n<\/jats:italic> = 3) acrosome responses (<jats:italic>p<\/jats:italic> &lt; 0.01). Five MR bulls were found in group A in comparison with two MR bulls in group B (57.1 vs 12.5%; <jats:italic>p<\/jats:italic> &lt; 0.05). Intensity of the acrosome response (response index) was significantly higher in bull group A compared with bull group B (7.0 vs 4.6, <jats:italic>p<\/jats:italic> &lt; 0.01). A positive correlation was recorded between response index and embryo rate (<jats:italic>r<\/jats:italic> = 0.668, <jats:italic>p<\/jats:italic> &lt; 0.01). In conclusion (<jats:italic>a<\/jats:italic>) the kinetics of spermatozoa response to heparin may be important for <jats:italic>in vitro<\/jats:italic> fertilization, bulls with a moderate response appearing to be most suitable for embryo production; (<jats:italic>b<\/jats:italic>) greater spermatozoa response to heparin was related to more effective embryo production.<\/jats:p>","DOI":"10.1017\/s096719940600356x","type":"journal-article","created":{"date-parts":[[2006,3,24]],"date-time":"2006-03-24T09:10:32Z","timestamp":1143191432000},"page":"63-69","source":"Crossref","is-referenced-by-count":3,"title":["A potential relationship between the acrosome response characteristics of bovine spermatozoa and their <i>in vitro<\/i> fertilizing ability"],"prefix":"10.1017","volume":"14","author":[{"given":"Zuzana","family":"Molnarova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie","family":"Machatkova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ladislav","family":"Machal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jindra","family":"Horakova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katerina","family":"Hanzalova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2006,3,24]]},"container-title":["Zygote"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S096719940600356X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,8]],"date-time":"2019-04-08T19:11:58Z","timestamp":1554750718000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S096719940600356X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,2]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2006,2]]}},"alternative-id":["S096719940600356X"],"URL":"https:\/\/doi.org\/10.1017\/s096719940600356x","relation":{},"ISSN":["0967-1994","1469-8730"],"issn-type":[{"value":"0967-1994","type":"print"},{"value":"1469-8730","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,2]]}}}