{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T08:08:30Z","timestamp":1778227710741,"version":"3.51.4"},"reference-count":34,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2009,11,26]],"date-time":"2009-11-26T00:00:00Z","timestamp":1259193600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Cryopreservation of gametes is an important tool in assisted reproduction programmes; long-term storage of oocytes or spermatozoa is necessary when <jats:italic>in vitro<\/jats:italic> fertilization (IVF) or artificial insemination is to be performed at a future date. Cryopreservation of epididymal spermatozoa offers a potential tool for rescuing genetic material from males of endangered populations. The objectives of this work were to: (1) examine sperm motility, viability, abnormality and acrosome integrity of frozen\u2013thawed domestic cat epididymal spermatozoa; and (2) evaluate the same cryopreservation method on wild feline spermatozoa, needed to preserve their genetic resources. Epididymides were collected from 20 domestic cats during routine neutering procedure and from two wild felines at autopsy. The sperm samples, diluted with 4% glycerol\/Tris\/egg yolk, were loaded into 0.25 ml mini-straws, exposed to nitrogen vapour and stored in liquid nitrogen. After 4 weeks, samples were thawed and re-evaluated. The quality of each fresh and frozen\u2013thawed sperm sample was tested by determining the motility (54.7 \u00b1 11.3% and 32 \u00b1 13.1% respectively for cat spermatozoa; 38.3 \u00b1 18.7% and 21.5 \u00b1 16.8% respectively for tiger spermatozoa), viability (74.3 \u00b1 8.6% and 45.2 \u00b1 9.4% respectively for cat spermatozoa; 42.4 \u00b1 14.5% and 33.5 \u00b1 12.9% respectively for wild felid spermatozoa), morphology and acrosomal status. The present study showed that feline epididymal spermatozoa can be frozen in egg-yolk extender with 4.0% glycerol in 0.25 ml straws. The procedure used in the present study for epididymal cat sperm cryopreservation may be applied to bank the genetic resources of wild felid species.<\/jats:p>","DOI":"10.1017\/s0967199409990256","type":"journal-article","created":{"date-parts":[[2009,11,27]],"date-time":"2009-11-27T20:43:46Z","timestamp":1259354626000},"page":"1-8","source":"Crossref","is-referenced-by-count":32,"title":["Cryopreservation of feline epididymal spermatozoa from dead and alive animals and its use in assisted reproduction"],"prefix":"10.1017","volume":"18","author":[{"given":"N.","family":"Cocchia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Ciani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"El-Rass","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Russo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Borzacchiello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Esposito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Montagnaro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Avallone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Tortora","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Lorizio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,11,26]]},"reference":[{"key":"S0967199409990256_ref32","first-page":"189","volume-title":"Effects of Low Temperatures on Biological Membranes","author":"Watson","year":"1981"},{"key":"S0967199409990256_ref31","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(84)90084-8"},{"key":"S0967199409990256_ref29","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0531.2002.00365.x"},{"key":"S0967199409990256_ref25","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.62.1163"},{"key":"S0967199409990256_ref24","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod61.1.188"},{"key":"S0967199409990256_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/S0011-2240(02)00035-4"},{"key":"S0967199409990256_ref15","volume-title":"Impiego Della Clortetraciclina Nella Valutazione Dell'integrita\u2019 Funzionale Degli Spermatozoi Epididimali Felini Congelati","author":"Marinoni","year":"2001"},{"key":"S0967199409990256_ref21","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod61.1.135"},{"key":"S0967199409990256_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.03.012"},{"key":"S0967199409990256_ref12","doi-asserted-by":"publisher","DOI":"10.2307\/1380997"},{"key":"S0967199409990256_ref10","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.69.869"},{"key":"S0967199409990256_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2007.01.008"},{"key":"S0967199409990256_ref30","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.02.011"},{"key":"S0967199409990256_ref5","first-page":"239","article-title":"Cold shock and its prevention by egg yolk in spermatozoa of the cat (Felis catus)","volume":"6","author":"Glover","year":"1985","journal-title":"Cryo Lett"},{"key":"S0967199409990256_ref18","unstructured":"Novell K. & Jackson P. (1996). Wild Cats: Status, Survey and Conservation Action Plan. IUCN-Report. Gland, Switzerland."},{"key":"S0967199409990256_ref4","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080400213"},{"key":"S0967199409990256_ref33","doi-asserted-by":"publisher","DOI":"10.1071\/RD9930639"},{"key":"S0967199409990256_ref11","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.17052"},{"key":"S0967199409990256_ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.10.022"},{"key":"S0967199409990256_ref16","first-page":"1","volume-title":"Development in Mammals","author":"Meizel","year":"1978"},{"key":"S0967199409990256_ref26","doi-asserted-by":"publisher","DOI":"10.1071\/RD05098"},{"key":"S0967199409990256_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2006.09.022"},{"key":"S0967199409990256_ref6","first-page":"297","article-title":"Comparative cryopreservation and capacitation of spermatozoa from epididymides and vasa deferentia of the domestic cat","volume":"47","author":"Hay","year":"1993","journal-title":"J. Reprod. Fertil. Suppl."},{"key":"S0967199409990256_ref20","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/S0015-0282(16)57672-9","article-title":"A time course study of capacitation and the acrosome reaction in human spermatozoa using a revised CTC pattern classification","volume":"64","author":"Perry","year":"1995","journal-title":"Fert. Steril."},{"key":"S0967199409990256_ref17","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1220463"},{"key":"S0967199409990256_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(98)00079-7"},{"key":"S0967199409990256_ref13","doi-asserted-by":"publisher","DOI":"10.1086\/303350"},{"key":"S0967199409990256_ref27","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.65.397"},{"key":"S0967199409990256_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(98)00200-3"},{"key":"S0967199409990256_ref34","first-page":"497","volume-title":"Encyclopedia of Reproduction","author":"Wildt","year":"1999"},{"key":"S0967199409990256_ref28","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.01.054"},{"key":"S0967199409990256_ref19","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012399770-8\/50006-X"},{"key":"S0967199409990256_ref2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod2.Supplement_2.128"},{"key":"S0967199409990256_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.04.005"}],"container-title":["Zygote"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0967199409990256","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,28]],"date-time":"2019-04-28T20:49:48Z","timestamp":1556484588000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0967199409990256\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,11,26]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,2]]}},"alternative-id":["S0967199409990256"],"URL":"https:\/\/doi.org\/10.1017\/s0967199409990256","relation":{},"ISSN":["0967-1994","1469-8730"],"issn-type":[{"value":"0967-1994","type":"print"},{"value":"1469-8730","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,11,26]]}}}