{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T08:45:16Z","timestamp":1648975516900},"reference-count":25,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2011,8,15]],"date-time":"2011-08-15T00:00:00Z","timestamp":1313366400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The aim of our study was to investigate the influence of vitrification on developmental rate and quality (total number of cells, number of blastomeres in inner cell mass (ICM) area, apoptotic index and embryo diameter) of transgenic (carrying an endogenous\u2013<jats:italic>hFVIII<\/jats:italic> or exogenous\u2013enhanced green fluorescent protein (<jats:italic>EGFP<\/jats:italic>) gene) rabbit embryos. <jats:italic>EGFP<\/jats:italic>-positive rabbit embryos were produced under <jats:italic>in vitro<\/jats:italic> conditions by the microinjection of foreign genes into the pronucleus of fertilized eggs. The transgenic rabbit embryos with the <jats:italic>hFVIII<\/jats:italic> gene were produced by mating homozygous transgenic rabbits and flushing at the single-cell stage. Developmental rate of vitrified\/thawed transgenic embryos that reached hatching blastocyst stage (68.00% and 69.00%) and differed significantly (<jats:italic>p<\/jats:italic> &lt; 0.001) from those in control embryos (100.00%). Significant difference (<jats:italic>p<\/jats:italic> &lt; 0.05) was found in total cell counts between control (117.00 \u00b1 36.00) and vitrified (141.00 \u00b1 34.80) hFVIII-positive embryos. The higher proportion of ICM cells (32.00%) and greatest embryo diameter (130.85 \u00b1 10.90) were found in the control group compared with the transgenic. Ratio of apoptotic cells was significantly higher (<jats:italic>p<\/jats:italic> &lt; 0.01) in the control group (2.50%) and vitrified EGFP-positive embryos (2.90%) compared with the vitrified, hFVIII-positive group of embryos (0.70%). Our results demonstrate that neither gene microinjection itself, nor exogenous (EGFP) and endogenous (hFVIII) gene expression interferes with developmental rate and quality of rabbit embryos. However, a combination of microinjection and vitrification significantly decreases (<jats:italic>p<\/jats:italic> &lt; 0.001) the survival rate of rabbit embryos.<\/jats:p>","DOI":"10.1017\/s0967199411000384","type":"journal-article","created":{"date-parts":[[2011,8,15]],"date-time":"2011-08-15T09:30:57Z","timestamp":1313400657000},"page":"53-58","source":"Crossref","is-referenced-by-count":1,"title":["Quality of rabbit vitrified\/thawed transgenic embryos"],"prefix":"10.1017","volume":"21","author":[{"given":"P.","family":"Chrenek","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Turanov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"J.","family":"Slame\u010dka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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