{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:09:11Z","timestamp":1762160951888},"reference-count":93,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2012,7,18]],"date-time":"2012-07-18T00:00:00Z","timestamp":1342569600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>During meiosis resumption, oocytes undergo a series of nuclear and cytosolic changes that prepare them for fertilization and that are referred to as oocyte maturation. These events are characterized by germinal vesicle breakdown (GVBD), chromatin condensation and spindle formation and, among cytosolic changes, organelle redistribution and maturation of Ca<jats:sup>2+<\/jats:sup>-release mechanisms. The progression of the meiotic cell cycle is regulated by M phase\/maturation-promoting factor (MPF) and mitogen-activated protein kinase (MAPK<jats:bold>)<\/jats:bold>. Changes in the levels of intracellular free Ca<jats:sup>2+<\/jats:sup>ion have also been implicated strongly in the triggering of the initiation of the M phase. Ca<jats:sup>2+<\/jats:sup>signals can be generated by Ca<jats:sup>2+<\/jats:sup>release from intracellular Ca<jats:sup>2+<\/jats:sup>stores (endoplasmic reticulum; ER) or by Ca<jats:sup>2+<\/jats:sup>influx from the extracellular space. In this sense, the L-type Ca<jats:sup>2+<\/jats:sup>channel plays an important role in the incorporation of Ca<jats:sup>2+<\/jats:sup>from the extracellular space. Two types of intracellular Ca<jats:sup>2+<\/jats:sup>receptor\/channels are known to mediate the intracellular Ca<jats:sup>2+<\/jats:sup>release from the ER lumen. The most abundant, the inositol 1,4,5-trisphosphate receptor (IP<jats:sub>3<\/jats:sub>R), and the other Ca<jats:sup>2+<\/jats:sup>channel, the ryanodine receptor (RyR), have also been reported to mediate Ca<jats:sup>2+<\/jats:sup>release in several oocytes. In amphibians, MPF and MAPK play a central role during oocyte maturation, controlling several events. However, no definitive relationships have been identified between Ca<jats:sup>2+<\/jats:sup>and MPF or MAPK. We investigated the participation of Ca<jats:sup>2+<\/jats:sup>in the spontaneous and progesterone-induced nuclear maturation in<jats:italic>Rhinella arenarum<\/jats:italic>oocytes and the effect of different pharmacological agents known to produce modifications in the Ca<jats:sup>2+<\/jats:sup>channels. We demonstrated that loading competent and incompetent oocytes with the intracellular calcium chelator BAPTA\/AM produced suppression of spontaneous and progesterone-induced GVBD. In our results, the capacity of progesterone to trigger meiosis reinitiation in<jats:italic>Rhinella<\/jats:italic>in the presence of L-type Ca<jats:sup>2+<\/jats:sup>channel blockers (nifedipine and lanthane) indicated that spontaneous and progesterone-induced maturation would be independent of extracellular calcium influx, but would be sensitive to intracellular Ca<jats:sup>2+<\/jats:sup>deprivation. As demonstrated by the effect of thimerosal and heparin in<jats:italic>Rhinella arenarum<\/jats:italic>, the intracellular increase in Ca<jats:sup>2+<\/jats:sup>during maturation is also mediated mainly by IP<jats:sub>3<\/jats:sub>R. In addition, our results using caffeine, an agonist of the RyR, could suggest that Ca<jats:sup>2+<\/jats:sup>release from ryanodine-sensitive stores is not essential for oocyte maturation in<jats:italic>Rhinella.<\/jats:italic>The decrease in MPF activity with NaVO<jats:sub>3<\/jats:sub>negatively affected the percentage of thimerosal-induced GVBD. This finding suggests that Ca<jats:sup>2+<\/jats:sup>release through the IP<jats:sub>3<\/jats:sub>R could be involved in the signalling pathway that induces MPF activation. However, the inhibition of MAP\/ERK kinase (MEK) by PD98128 or P90 by geldanamycin produced a significant decrease in the percentages of GVBD induced by thimerosal. This finding suggests that Ca<jats:sup>2+<\/jats:sup>release<jats:italic>per se<\/jats:italic>cannot bypass the inhibition of the MAPK activity.<\/jats:p>","DOI":"10.1017\/s0967199412000287","type":"journal-article","created":{"date-parts":[[2012,7,18]],"date-time":"2012-07-18T10:18:32Z","timestamp":1342606712000},"page":"110-123","source":"Crossref","is-referenced-by-count":9,"title":["Participation of IP<sub>3<\/sub>R, RyR and L-type Ca<sup>2+<\/sup>channel in the nuclear maturation of<i>Rhinella arenarum<\/i>oocytes"],"prefix":"10.1017","volume":"22","author":[{"given":"G. S\u00e1nchez","family":"Toranzo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.C. Gramajo","family":"B\u00fchler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.I.","family":"B\u00fchler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2012,7,18]]},"reference":[{"key":"S0967199412000287_ref20","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5365.895"},{"key":"S0967199412000287_ref90","doi-asserted-by":"crossref","first-page":"2645","DOI":"10.1242\/dev.121.8.2645","article-title":"The existence of inositol 1,4,5-tris-phosphate and ryanodine receptors in mature bovine oocytes","volume":"121","author":"Yue","year":"1995","journal-title":"Development"},{"key":"S0967199412000287_ref11","doi-asserted-by":"crossref","first-page":"9404","DOI":"10.1016\/S0021-9258(19)70576-6","article-title":"Xenopus laevis oocyte calmodulin in the process of meiotic maturation","volume":"255","author":"Cartaud","year":"1980","journal-title":"J. Biol. Chem."},{"key":"S0967199412000287_ref33","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.111.1.183"},{"key":"S0967199412000287_ref56","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1993.1168"},{"key":"S0967199412000287_ref77","doi-asserted-by":"publisher","DOI":"10.1016\/S0012-1606(89)80034-X"},{"key":"S0967199412000287_ref34","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402650110"},{"key":"S0967199412000287_ref53","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-13443-2_13"},{"key":"S0967199412000287_ref47","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.26.15821"},{"key":"S0967199412000287_ref58","first-page":"1309","article-title":"Motificationes precoces des properties electriques de la membrana plasmique des oocytes de Xenopus laevis. Et cours de la reinitiation meiotique par la progesterona, la paramercuri benzoate (PCMB) on l'ionophore","volume":"282","author":"Moreau","year":"1976","journal-title":"C. R. Acad. Sci. Paris"},{"key":"S0967199412000287_ref44","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1242\/dev.127.3.631","article-title":"Sperm-induced calcium oscillations at fertilisation in ascidians are controlled by cyclin B1-dependent kinase activity","volume":"127","author":"Levasseur","year":"2000","journal-title":"Development"},{"key":"S0967199412000287_ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpc.2004.06.010"},{"key":"S0967199412000287_ref16","doi-asserted-by":"crossref","first-page":"11726","DOI":"10.1016\/S0021-9258(19)38457-1","article-title":"Effect of inositol trisphosphate and calcium on oscillating elevations of intracellular calcium in Xenopus oocytes","volume":"265","author":"DeLisle","year":"1990","journal-title":"J. Biol. Chem."},{"key":"S0967199412000287_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/S1642-431X(12)60001-1"},{"key":"S0967199412000287_ref29","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402580111"},{"key":"S0967199412000287_ref15","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20404"},{"key":"S0967199412000287_ref2","doi-asserted-by":"crossref","first-page":"2233","DOI":"10.1242\/dev.121.7.2233","article-title":"Regulation of mouse egg activation: presence of ryanodine receptors and effects of microinjected ryanodine and cyclic ADP ribose on uninseminated and inseminated eggs","volume":"121","author":"Ayabe","year":"1995","journal-title":"Development"},{"key":"S0967199412000287_ref82","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.117.4.799"},{"key":"S0967199412000287_ref43","doi-asserted-by":"publisher","DOI":"10.1126\/science.2011747"},{"key":"S0967199412000287_ref36","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.1994.Supplement_18.17"},{"key":"S0967199412000287_ref65","doi-asserted-by":"publisher","DOI":"10.1016\/S1096-4959(01)00344-X"},{"key":"S0967199412000287_ref46","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod57.5.1123"},{"key":"S0967199412000287_ref8","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1994.1118"},{"key":"S0967199412000287_ref48","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1152\/ajpcell.1998.275.1.C179","article-title":"Effects of divalent cations on single-channel conduction properties of Xenopus c receptor","volume":"275","author":"Mak","year":"1998","journal-title":"Am. J. Physiol."},{"key":"S0967199412000287_ref30","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a138240"},{"key":"S0967199412000287_ref24","doi-asserted-by":"publisher","DOI":"10.1126\/science.8392748"},{"key":"S0967199412000287_ref39","doi-asserted-by":"publisher","DOI":"10.1126\/science.278.5345.1940"},{"key":"S0967199412000287_ref76","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1995.1239"},{"key":"S0967199412000287_ref55","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(92)81090-9"},{"key":"S0967199412000287_ref25","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1242\/jcs.107.1.275","article-title":"Histone H1 kinase activity, germinal vesicle breakdown and M phase entry in mouse oocytes","volume":"107","author":"Gavin","year":"1994","journal-title":"J. Cell Sci."},{"key":"S0967199412000287_ref27","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1095\/biolreprod57.5.1245","article-title":"Calcium, calcium release receptors, and meiotic resumption in bovine oocytes","volume":"57","author":"He","year":"1997","journal-title":"Biol. Reprod."},{"key":"S0967199412000287_ref31","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(82)90011-2"},{"key":"S0967199412000287_ref75","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20764"},{"key":"S0967199412000287_ref61","doi-asserted-by":"publisher","DOI":"10.1038\/313141a0"},{"key":"S0967199412000287_ref42","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20162"},{"key":"S0967199412000287_ref87","doi-asserted-by":"publisher","DOI":"10.1016\/0039-9140(93)80149-L"},{"key":"S0967199412000287_ref70","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199410000456"},{"key":"S0967199412000287_ref49","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1401660309"},{"key":"S0967199412000287_ref68","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90458-2"},{"key":"S0967199412000287_ref5","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod61.4.1050"},{"key":"S0967199412000287_ref62","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199400005116"},{"key":"S0967199412000287_ref63","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402170312"},{"key":"S0967199412000287_ref14","doi-asserted-by":"crossref","first-page":"C1485","DOI":"10.1152\/ajpcell.1994.266.6.C1485","article-title":"Structure and function of ryanodine receptors","volume":"266","author":"Coronado","year":"1994","journal-title":"Am. J. Physiol."},{"key":"S0967199412000287_ref85","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1993.1192"},{"key":"S0967199412000287_ref52","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1996.0322"},{"key":"S0967199412000287_ref73","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod10.2.150"},{"key":"S0967199412000287_ref60","doi-asserted-by":"crossref","first-page":"5597","DOI":"10.1002\/j.1460-2075.1995.tb00247.x","article-title":"Newly synthesized protein(s) must associate with p34cdc32 to activate MAP kinase and MPF during progesterone\u2013induced maturation of Xenopus oocytes","volume":"14","author":"Nebreda","year":"1995","journal-title":"EMBO J."},{"key":"S0967199412000287_ref26","doi-asserted-by":"publisher","DOI":"10.1261\/rna.1195709"},{"key":"S0967199412000287_ref88","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1242\/dev.108.4.525","article-title":"Calcium and cell cycle control","volume":"108","author":"Whitaker","year":"1990","journal-title":"Development"},{"key":"S0967199412000287_ref32","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1993.1148"},{"key":"S0967199412000287_ref71","doi-asserted-by":"publisher","DOI":"10.1038\/262289a0"},{"key":"S0967199412000287_ref59","doi-asserted-by":"publisher","DOI":"10.1210\/endo.141.6.7510"},{"key":"S0967199412000287_ref67","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0660001"},{"key":"S0967199412000287_ref58a","doi-asserted-by":"publisher","DOI":"10.1097\/00004424-197811000-00021"},{"key":"S0967199412000287_ref84","doi-asserted-by":"publisher","DOI":"10.4161\/cc.4.3.1532"},{"key":"S0967199412000287_ref13","doi-asserted-by":"publisher","DOI":"10.1038\/370065a0"},{"key":"S0967199412000287_ref83","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402050213"},{"key":"S0967199412000287_ref79","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.107.063693"},{"key":"S0967199412000287_ref10","doi-asserted-by":"crossref","first-page":"3507","DOI":"10.1242\/dev.120.12.3507","article-title":"Spatiotemporal dynamics of intracellular [Ca2+]i oscillations during the growth and meiotic maturation of mouse oocytes","volume":"120","author":"Carroll","year":"1994","journal-title":"Development"},{"key":"S0967199412000287_ref38","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90142-D"},{"key":"S0967199412000287_ref22","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod53.4.766"},{"key":"S0967199412000287_ref35","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402440116"},{"key":"S0967199412000287_ref23","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1993.1059"},{"key":"S0967199412000287_ref12","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.10.4730"},{"key":"S0967199412000287_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(77)90018-1"},{"key":"S0967199412000287_ref41","doi-asserted-by":"publisher","DOI":"10.1126\/science.8392749"},{"key":"S0967199412000287_ref45","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1120120103"},{"key":"S0967199412000287_ref86","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1401930313"},{"key":"S0967199412000287_ref78","doi-asserted-by":"publisher","DOI":"10.1038\/332364a0"},{"key":"S0967199412000287_ref7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.12.4362"},{"key":"S0967199412000287_ref57","first-page":"293","article-title":"Electrical properties of the starfish oocyte membranes","volume":"72","author":"Moreau","year":"1976","journal-title":"J Physiol (Paris)"},{"key":"S0967199412000287_ref28","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/3.11.965"},{"key":"S0967199412000287_ref89","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod56.1.253"},{"key":"S0967199412000287_ref4","doi-asserted-by":"publisher","DOI":"10.1038\/361315a0"},{"key":"S0967199412000287_ref21","doi-asserted-by":"crossref","first-page":"3111","DOI":"10.1002\/j.1460-2075.1993.tb05980.x","article-title":"The MO15 gene encodes the catalytic subunit of a protein kinase that activates cdc2 and other cyclin-dependent kinases (CDKs) through phosphorylation of Thr161 and its homologues","volume":"12","author":"Fesquet","year":"1993","journal-title":"EMBO J."},{"key":"S0967199412000287_ref54","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.02270"},{"key":"S0967199412000287_ref9","doi-asserted-by":"crossref","first-page":"11196","DOI":"10.1016\/S0021-9258(19)49895-5","article-title":"Spontaneous cytosolic calcium oscillations driven by inositol trisphosphate occur during in vitro maturation of mouse oocytes","volume":"267","author":"Carroll","year":"1992","journal-title":"J. Biol. Chem."},{"key":"S0967199412000287_ref81","doi-asserted-by":"publisher","DOI":"10.1042\/bj2870079"},{"key":"S0967199412000287_ref51","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-0436.1977.tb01518.x"},{"key":"S0967199412000287_ref91","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod58.2.608"},{"key":"S0967199412000287_ref40","doi-asserted-by":"crossref","first-page":"3053","DOI":"10.1002\/j.1460-2075.1989.tb08456.x","article-title":"MPF from starfish oocytes at first meiotic metaphase is a heterodimer containing one molecule of cdc2 and one molecule of cyclin B","volume":"8","author":"Labb\u00e9","year":"1989","journal-title":"EMBO J."},{"key":"S0967199412000287_ref92","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402720505"},{"key":"S0967199412000287_ref37","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199404002564"},{"key":"S0967199412000287_ref50","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1401770202"},{"key":"S0967199412000287_ref66","doi-asserted-by":"publisher","DOI":"10.1038\/315147a0"},{"key":"S0967199412000287_ref18","doi-asserted-by":"publisher","DOI":"10.1038\/208349a0"},{"key":"S0967199412000287_ref93","doi-asserted-by":"publisher","DOI":"10.1017\/S096719940400259X"},{"key":"S0967199412000287_ref80","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(91)80110-O"},{"key":"S0967199412000287_ref6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.44.27847"},{"key":"S0967199412000287_ref17","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199400002859"},{"key":"S0967199412000287_ref69","doi-asserted-by":"publisher","DOI":"10.1038\/342512a0"},{"key":"S0967199412000287_ref74","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.66.1.86"},{"key":"S0967199412000287_ref64","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.17.5037"}],"container-title":["Zygote"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0967199412000287","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,22]],"date-time":"2022-01-22T01:28:47Z","timestamp":1642814927000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0967199412000287\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,7,18]]},"references-count":93,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,5]]}},"alternative-id":["S0967199412000287"],"URL":"https:\/\/doi.org\/10.1017\/s0967199412000287","relation":{},"ISSN":["0967-1994","1469-8730"],"issn-type":[{"value":"0967-1994","type":"print"},{"value":"1469-8730","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,7,18]]}}}