{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T19:00:44Z","timestamp":1648666844813},"reference-count":37,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2013,5,17]],"date-time":"2013-05-17T00:00:00Z","timestamp":1368748800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Natural resistance-associated macrophage protein 1 (Nramp1) plays an important role in restraining the growth of intracellular pathogens within macrophages. In this study,<jats:italic>Nramp1<\/jats:italic>cDNA was cloned from Qinchuan cattle and its anti-bacterial activity was demonstrated as being able to significantly inhibit the growth of<jats:italic>Salmonella abortusovis<\/jats:italic>and<jats:italic>Brucella abortus<\/jats:italic>in macrophages. Calf fibroblasts stably transfected with pSP\u2013NRAMP1\u2013HA vector were used to reconstruct bovine embryos by somatic cell nuclear transfer (SCNT). Reconstructed embryos were maturated<jats:italic>in vitro<\/jats:italic>and the blastocyst formation rate (14.0%) was similar to that of control embryos (14.5%). Transgenic blastocysts were transplanted into 43 recipient cattle, of which 14 recipients became pregnant as evidenced by non-return estrus and by rectal palpation. One fetus was aborted after 6\u00bd months of pregnancy and transgene integration was confirmed by semi-quantitative polymerase chain reaction. Together, this study showed that bovine Nramp1 retains biological function against the growth of intracellular bacteria and can be used to reconstruct embryos and produce<jats:italic>Nramp1<\/jats:italic>transgenic cattle, which may benefit the animal and enhance their ability to prevent attack by intracellular pathogens.<\/jats:p>","DOI":"10.1017\/s096719941300021x","type":"journal-article","created":{"date-parts":[[2013,5,17]],"date-time":"2013-05-17T13:06:58Z","timestamp":1368796018000},"page":"83-92","source":"Crossref","is-referenced-by-count":2,"title":["Functional analysis of bovine Nramp1 and production of transgenic cloned embryos<i>in vitro<\/i>"],"prefix":"10.1017","volume":"23","author":[{"given":"Xiang","family":"Cheng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoli","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yajun","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Deng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoling","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huayan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2013,5,17]]},"reference":[{"key":"S096719941300021X_ref33","doi-asserted-by":"publisher","DOI":"10.1084\/jem.182.3.655"},{"key":"S096719941300021X_ref34","first-page":"441","article-title":"Construction and targeting study of eukaryotic expression vector modulated by a macrophage-specific promoter","volume":"24","author":"Wen","year":"2008","journal-title":"Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi"},{"key":"S096719941300021X_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2007.05.059"},{"key":"S096719941300021X_ref31","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00153-07"},{"key":"S096719941300021X_ref8","first-page":"685","article-title":"The cloning and its expression in the cells and the tissues of the bovine natural resistance-associated macrophage protein 1 gene isoform (NRAMP1-ISO)","volume":"19","author":"Cheng","year":"2011","journal-title":"Chin. 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