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Details of the unique process of the interaction between egg jelly and sperm in these species is still unclear. The current study showed the fine structure of egg jelly in the newt<jats:italic>Cynops pyrrhogaster<\/jats:italic>, a urodele species, revealing that its outer surface was covered by a sheet-like structure of approximately 0.29 \u03bcm in thickness. Granules of approximately 2 \u03bcm in diameter with small particles of approximately 54 nm were attached to its surface and distributed inhomogeneously just beneath the sheet-like structure. Emission spectrometry revealed that the Ca<jats:sup>2+<\/jats:sup>concentration was maintained at a high level compared with that of the blood plasma and the vas deferens fluid, suggesting that egg jelly is a reliable source of Ca<jats:sup>2+<\/jats:sup>for the sperm\u2013egg interaction. Blockers of the T-type voltage-dependent Ca<jats:sup>2+<\/jats:sup>channel (VDCC), but not the L-type VDCC, inhibited both AR and initiation of sperm motility. Conversely, Ni<jats:sup>+<\/jats:sup>, which affects the \u03b1<jats:sub>1 H<\/jats:sub>subunit of T-type VDCC, only inhibited the initiation of sperm motility. These data suggest that, in response to ARIS and SMIS, sequential gating of distinct Ca<jats:sup>2+<\/jats:sup>channels occurs in the AR, followed by the initiation of sperm motility on the surface of the egg jelly in<jats:italic>C. pyrrhogaster<\/jats:italic>at fertilization.<\/jats:p>","DOI":"10.1017\/s0967199413000609","type":"journal-article","created":{"date-parts":[[2013,12,20]],"date-time":"2013-12-20T10:34:25Z","timestamp":1387535665000},"page":"342-351","source":"Crossref","is-referenced-by-count":11,"title":["Contribution of different Ca<sup>2+<\/sup>channels to the acrosome reaction-mediated initiation of sperm motility in the newt<i>Cynops pyrrhogaster<\/i>"],"prefix":"10.1017","volume":"23","author":[{"given":"Eriko","family":"Takayama-Watanabe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroto","family":"Ochiai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shunpei","family":"Tanino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiko","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2013,12,20]]},"reference":[{"key":"S0967199413000609_ref034","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.108.074039"},{"key":"S0967199413000609_ref028","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199412000226"},{"key":"S0967199413000609_ref016","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.75.1.144"},{"key":"S0967199413000609_ref020","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402650315"},{"key":"S0967199413000609_ref009","doi-asserted-by":"publisher","DOI":"10.1002\/jez.10019"},{"key":"S0967199413000609_ref008","doi-asserted-by":"publisher","DOI":"10.1002\/jez.376"},{"key":"S0967199413000609_ref010","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012311629-1\/50005-X"},{"key":"S0967199413000609_ref012","first-page":"1","article-title":"Role of oviductal secretions in mediating gamete fusion in anuran amphibians","volume":"4","author":"Katagiri","year":"1987","journal-title":"Zool. 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