{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T10:29:05Z","timestamp":1762252145746},"reference-count":25,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2014,11,26]],"date-time":"2014-11-26T00:00:00Z","timestamp":1416960000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Mature oocytes are arrested in metaphase II due to the presence of high levels of active maturation promoting factor (MPF). After fertilization, active MPF levels decline abruptly, enabling oocytes to complete meiosis II. One of the first and universal events of oocyte activation is an increase in cytosolic Ca<jats:sup>2+<\/jats:sup>that would be responsible for MPF inactivation. Mature oocytes can also be activated by parthenogenetic activation. The aims of this work are to test the ability of dehydroleucodine (DhL) and its hydrogenated derivative 11,13-dihydro-dehydroleucodine (2H-DhL) to induce chemical activation in amphibian oocytes and to study the participation of calcium in the process. Results indicated that DhL and 2H-DhL induced oocyte activation in a dose-dependent manner. After 90 min of treatment, DhL 36 \u03bcM was able to induce 95% activation, while 2H-DhL 36 \u03bcM was less active, with only 40% activation. Our results suggest that DhL induced the inhibition of MPF activity, probably by an increase in intracellular Ca<jats:sup>2+<\/jats:sup>concentration. Extracellular Ca<jats:sup>2+<\/jats:sup>would not be significant, although Ca<jats:sup>2+<\/jats:sup>release from intracellular stores is critical. In this sense, IP<jats:sub>3<\/jats:sub>Rs and RyRs were involved in the Ca<jats:sup>2+<\/jats:sup>transient induced by lactones. In this species, RyRs appears to be the largest contributor to Ca<jats:sup>2+<\/jats:sup>release in DhL-induced activation. Although more studies are needed on the mechanism of action through which these lactones induce oocyte activation in<jats:italic>Rhinella arenarum<\/jats:italic>, the results of this research provide interesting perspectives for the use of these lactones as chemical activators in<jats:italic>in vitro<\/jats:italic>fertilization and cloning.<\/jats:p>","DOI":"10.1017\/s0967199414000641","type":"journal-article","created":{"date-parts":[[2014,11,26]],"date-time":"2014-11-26T07:33:32Z","timestamp":1416987212000},"page":"924-932","source":"Crossref","is-referenced-by-count":1,"title":["Chemical activation in<i>Rhinella arenarum<\/i>oocytes: effect of dehydroleucodine (DhL) and its hydrogenated derivative (2H-DhL)"],"prefix":"10.1017","volume":"23","author":[{"given":"M.F.","family":"Medina","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.I.","family":"B\u00fchler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"S\u00e1nchez-Toranzo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2014,11,26]]},"reference":[{"key":"S0967199414000641_ref018","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199407004212"},{"key":"S0967199414000641_ref015","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1993.1168"},{"key":"S0967199414000641_ref013","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1007\/s007090200008","article-title":"Sesquiterpene lactone dehydroleucodine selectively induces transient arrest in G2 in Allium cepa root meristematic cells","volume":"219","author":"L\u00f3pez","year":"2002","journal-title":"Protoplasma"},{"key":"S0967199414000641_ref023","doi-asserted-by":"publisher","DOI":"10.1089\/clo.2005.7.306"},{"key":"S0967199414000641_ref010","doi-asserted-by":"publisher","DOI":"10.1021\/jm00091a013"},{"key":"S0967199414000641_ref011","doi-asserted-by":"crossref","first-page":"17624","DOI":"10.1016\/S0021-9258(19)37088-7","article-title":"Thapsigargin activates a calcium influx pathway in the unfertilized mouse egg and suppresses repetitive calcium transients in the fertilized egg","volume":"267","author":"Kline","year":"1992","journal-title":"J. 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