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The PI3K inhibitor wortmannin abrogates recombinant human (rh)-insulin action on histone H1 kinase activation and meiotic G2\u2013M1 transition in denuded and follicle-enclosed oocytes<jats:italic>in vitro.<\/jats:italic>While the translational inhibitor cycloheximide attenuates rh-insulin action, priming with transcriptional blocker actinomycin D prevents insulin-stimulated maturational response appreciably, albeit in low amounts. Compared with rh-insulin, human chorionic gonadotrophin (hCG) stimulation of follicle-enclosed oocytes<jats:italic>in vitro<\/jats:italic>triggers a sharp increase in 17\u03b1,20\u03b2-dihydroxy-4-pregnen-3-one (17\u03b1,20\u03b2-DHP) secreted in the incubation medium at 12 h. Interestingly, the insulin, but not the hCG-induced, maturational response shows less susceptibility to steroidogenesis inhibitors, trilostane or<jats:sc>dl<\/jats:sc>-aminoglutethimide. In addition, priming with phosphodiesterase inhibitor, 3-isobutyl-1-methylxanthine (IBMX) or cell-permeable dbcAMP or adenylyl cyclase activator forskolin reverses the action of insulin on meiotic G2\u2013M1 transition. Conversely, the adenylyl cyclase inhibitor, SQ 22536, or PKA inhibitor H89 promotes the resumption of meiosis alone and further potentiates the GVBD response in the presence of rh-insulin. Furthermore, insulin-mediated meiotic maturation involves the down-regulation of endogenous protein kinase A (PKA) activity in a manner sensitive to PI3K activation, suggesting potential involvement of a cross-talk between cAMP\/PKA and insulin-mediated signalling cascade in catfish oocytes<jats:italic>in vitro.<\/jats:italic>Taken together, these results suggest that rh-insulin regulation of the maturational response in<jats:italic>C. batrachus<\/jats:italic>oocytes involves down-regulation of PKA, synthesis of cyclin B, and histone H1 kinase activation and demonstrates reduced sensitivity to steroidogenesis and transcriptional inhibition.<\/jats:p>","DOI":"10.1017\/s0967199415000015","type":"journal-article","created":{"date-parts":[[2015,2,24]],"date-time":"2015-02-24T09:45:01Z","timestamp":1424771101000},"page":"181-194","source":"Crossref","is-referenced-by-count":8,"title":["Regulation of recombinant human insulin-induced maturational events in<i>Clarias batrachus<\/i>(L.) oocytes<i>in vitro<\/i>"],"prefix":"10.1017","volume":"24","author":[{"given":"Sudip","family":"Hajra","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debabrata","family":"Das","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pritha","family":"Ghosh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soumojit","family":"Pal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Poulomi","family":"Nath","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sudipta","family":"Maitra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2015,2,24]]},"reference":[{"key":"S0967199415000015_ref021","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601431"},{"key":"S0967199415000015_ref061","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.036251"},{"key":"S0967199415000015_ref025","doi-asserted-by":"publisher","DOI":"10.1006\/gcen.1994.1126"},{"key":"S0967199415000015_ref044","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2010.04.021"},{"key":"S0967199415000015_ref022","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(83)90213-0"},{"key":"S0967199415000015_ref013","first-page":"641","article-title":"Insulin-like growth factors and ovarian follicular development","volume":"13","author":"Giudice","year":"1992","journal-title":"Endocr. 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