{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:57:31Z","timestamp":1787237851091,"version":"build-2736575974"},"reference-count":30,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The aim of this study was to evaluate the performance of cavy (\n                    <jats:italic>Galea spixii<\/jats:italic>\n                    ) epididymal sperm following addition to TES or TRIS extenders and using a thermal resistance test (TRT), as well as fluorescence analysis as a complementary method to predict the viability of these gametes. Nine testicle\u2013epididymis complexes were used for sperm collection using a flotation method. Epididymis tails were sliced and one was immersed in 3 ml of TRIS buffer, and the other in 3 ml of TES, for 5 min. After sperm recovery, the samples were subjected to a TRT which involved incubation in a water bath at 37\u00b0C for 3 h. During incubation, sample parameters were assessed at 0, 15, 30, 60, 90, 120, 150 or 180 min intervals. Results indicated that the TRIS diluent was more efficient than TES (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05) for the maintenance of sperm parameters in Spix's yellow-toothed cavies over the whole TRT, maintaining sperm longevity for an extended time. In conclusion, we indicate the use of TRIS diluent for recovery and maintenance of longevity of epididymal sperm from cavies (\n                    <jats:italic>G. spixii<\/jats:italic>\n                    ).\n                  <\/jats:p>","DOI":"10.1017\/s0967199417000016","type":"journal-article","created":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T02:58:40Z","timestamp":1488337120000},"page":"176-182","source":"Crossref","is-referenced-by-count":5,"title":["Comparison of different extenders on the recovery and longevity of epididymal sperm from Spix's yellow-toothed cavies (\n                    <i>Galea spixii<\/i>\n                    Wagler, 1831)"],"prefix":"10.1017","volume":"25","author":[{"given":"Andr\u00e9ia Maria","family":"da Silva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia Cunha","family":"Sousa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00edvia Batista","family":"Campos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Artur Brilhante","family":"Bezerra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur Emannuel","family":"de Ara\u00fajo Lago","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moacir Franco","family":"de Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre Rodrigues","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2017,3,1]]},"reference":[{"key":"S0967199417000016_ref025","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-736X2016000800015"},{"key":"S0967199417000016_ref024","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.09.010"},{"key":"S0967199417000016_ref016","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5288(98)90132-1"},{"key":"S0967199417000016_ref027","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1501\/Vetfak_0000002658","article-title":"Influence of extenders and cooling rates on epididymal sperm of Lewis rat strain","volume":"62","author":"Varisli","year":"2015","journal-title":"Ankara. \u00dcniv. Vet. Fak. Derg"},{"key":"S0967199417000016_ref017","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.10111"},{"key":"S0967199417000016_ref030","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.18145"},{"key":"S0967199417000016_ref018","unstructured":"Rodrigues B.A. (1997). Efeito do diluidor \u00e0 base de albumina s\u00e9rica bovina (BSA) sobre a viabilidade in vitro do s\u00eamen canino criopreservado. [Effect of the extender based in bovine serum albumine (BSA) on the in vitro viability of canine frozen semen.] Master's Thesis in Veterinary Medicine \u2013 Post-graduation Course in Veterinary Science at the Federal University of Rio Grande do Sul, Brazil. 176 pp."},{"key":"S0967199417000016_ref003","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-09352010000300006"},{"key":"S0967199417000016_ref026","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-736X2013000800014"},{"key":"S0967199417000016_ref006","first-page":"536","article-title":"Avalia\u00e7\u00e3o da integridade de membrana em espermatoz\u00f3ide bovino criopreservado para prever o \u00edndice de prenhez","volume":"12","author":"Emerick","year":"2011","journal-title":"Ci. 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