{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,29]],"date-time":"2024-06-29T03:40:17Z","timestamp":1719632417240},"reference-count":54,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2017,11,28]],"date-time":"2017-11-28T00:00:00Z","timestamp":1511827200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>We produced a new chemical compound based on methylxanthines and polyphenols (CCMP) present in the chemical matrix of guaran\u00e1 (<jats:italic>Paullinia cupana<\/jats:italic>), a seed extract with antioxidant properties. After supplementation with the standard extract of resveratrol, a well documented antioxidant found in other plant sources, we investigated whether this resveratrol-enriched compound could improve sperm viability and modulate differentially reactive oxygen species (ROS) and nitric oxide (NO) levels in thawed sperm. Sperm samples obtained from healthy young donors were treated with different concentrations of guaran\u00e1 extract (0.1, 1, 5 or 10 mg\/ml) and cells were frozen at \u221280\u00b0C for 24 h. In addition, the potential protective effects of guaran\u00e1 treatment on sperm treated with pro-oxidant compound (200 \u00b5M hydrogen peroxide, H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) were assessed. Samples were also exposed to three concentrations of CCMP before being frozen in liquid nitrogen (\u2212196\u00b0C) or in an ultrafreezer (\u221280\u00b0C) for 24 h, and both pre-freezing and post-thaw measurements of viability and oxidative stress were performed. Guaran\u00e1 supplementation at 10 mg\/ml significantly increased post-thaw viability and decreased oxidative metabolism of the sperm. Moreover, selected concentrations of CCMP improved viability and oxidative metabolism in sperm samples pre-freezing. Furthermore, CCMP showed cryoprotective activity by increasing viability and decreasing oxidative stress in post-thaw samples. In summary, these findings suggested that CCMP supplementation acts as a cryoprotectant to modulate ROS and NO levels in thawed sperm. CCMP could be used to enhance sperm quality and reproductive success.<\/jats:p>","DOI":"10.1017\/s0967199417000600","type":"journal-article","created":{"date-parts":[[2017,11,28]],"date-time":"2017-11-28T05:26:12Z","timestamp":1511846772000},"page":"719-730","source":"Crossref","is-referenced-by-count":3,"title":["A chemical compound based on methylxanthine\u2013polyphenols lowers nitric oxide levels and increases post-thaw human sperm viability"],"prefix":"10.1017","volume":"25","author":[{"given":"Cleiton","family":"Werner","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francine Carla","family":"Cadon\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivana Beatrice M\u00e2nica","family":"da Cruz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliza Ribas da Silveira","family":"Fl\u00f4res","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alencar Kolinski","family":"Machado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mara Rejane","family":"Fantinel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grazielle Castagna Cezimbra","family":"Weis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles Elias","family":"Assmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Audrei de Oliveira","family":"Alves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz da Silva Rosa","family":"Bonadiman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Euler Esteves","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco Aur\u00e9lio Echart","family":"Montano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2017,11,28]]},"reference":[{"key":"S0967199417000600_ref050","doi-asserted-by":"publisher","DOI":"10.1071\/RD9930639"},{"key":"S0967199417000600_ref045","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-2115(99)00255-9"},{"key":"S0967199417000600_ref051","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2004.03.002"},{"key":"S0967199417000600_ref053","doi-asserted-by":"publisher","DOI":"10.1038\/aja.2010.182"},{"key":"S0967199417000600_ref016","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2012.12.007"},{"key":"S0967199417000600_ref026","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(99)00239-3"},{"key":"S0967199417000600_ref019","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(00)01494-1"},{"key":"S0967199417000600_ref029","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1002\/ptr.3437","article-title":"Habitual intake of guaran\u00e1 and metabolic morbidities: an epidemiological study of an elderly Amazonian population","volume":"25","author":"Krewer","year":"2011","journal-title":"Phytother. 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