{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T22:54:54Z","timestamp":1789772094253,"version":"4.0.1"},"reference-count":41,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2018,9,17]],"date-time":"2018-09-17T00:00:00Z","timestamp":1537142400000},"content-version":"unspecified","delay-in-days":47,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The aim of this study was to establish a functional freezing\u2013thawing protocol for epididymal sperm of collared peccaries (<jats:italic>Pecari tajacu<\/jats:italic> L., 1758) by comparing different extenders. The epididymal sperm from 12 sexually mature males was recovered by retrograde flushing using Tris-based or coconut water-based (ACP\u00ae-116c) extenders. After initial evaluation, samples were diluted and frozen with the same extenders to which 20% egg yolk and 6% glycerol were added. After 2 weeks, thawing was performed at 37\u00b0C\/60 s and sperm motility, vigour, morphology, functional membrane integrity, sperm viability, sperm plasma membrane integrity, and a computer-assisted semen analysis (CASA) were assessed. In addition, to evaluate the survival of frozen\u2013thawed sperm, a thermal resistance test (TRT) was executed. Samples preserved using Tris were in better condition compared with those preserved using ACP<jats:sup>\u00ae<\/jats:sup>, showing higher values for most assessments performed, including CASA and the TRT (<jats:italic>P<\/jats:italic>&lt;0.05). After determining Tris to be the better of the two extenders, additional samples were thawed using different thawing rates (37\u00b0C\/60 s, 55\u00b0C\/7 s, 70\u00b0C\/8 s). Sperm thawed at 37\u00b0C\/60 s had the greatest preservation (<jats:italic>P<\/jats:italic>&lt;0.05) of viability (54.1 \u00b1 5.9%) and functional membrane integrity (43.2 \u00b1 5.4%), and had higher values for various CASA parameters. In conclusion, we suggest the use of a Tris-based extender added to egg yolk and glycerol for the cryopreservation of epididymal sperm obtained from collared peccaries. In order to achieve better post-thawing sperm quality, we suggest that samples should be thawed at 37\u00b0C\/60 s.<\/jats:p>","DOI":"10.1017\/s0967199418000230","type":"journal-article","created":{"date-parts":[[2018,9,17]],"date-time":"2018-09-17T04:14:07Z","timestamp":1537157647000},"page":"301-307","source":"Crossref","is-referenced-by-count":7,"title":["Cryopreservation of collared peccary (<i>Pecari tajacu<\/i> L., 1758) epididymal sperm using extenders based on Tris and powdered coconut water (ACP\u00ae-116c)"],"prefix":"10.1017","volume":"26","author":[{"given":"Jos\u00e9 A. B.","family":"Bezerra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9ia M.","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia C","family":"Sousa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00edvia B.","family":"Campos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c9rica C. G.","family":"Praxedes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luana G. P.","family":"Bezerra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thib\u00e9rio S.","family":"Castelo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana L. P.","family":"Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6027-1062","authenticated-orcid":false,"given":"Alexandre R.","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2018,9,17]]},"reference":[{"key":"S0967199418000230_ref15","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12755"},{"key":"S0967199418000230_ref20","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-09352013000300010"},{"key":"S0967199418000230_ref10","first-page":"257","article-title":"Use of the powdered coconut water (ACP-106\u00ae) for cryopreservation of canine spermatozoa","volume":"2","author":"Cardoso","year":"2005","journal-title":"Anim. Reprod."},{"key":"S0967199418000230_ref29","doi-asserted-by":"publisher","DOI":"10.1111\/azo.12177"},{"key":"S0967199418000230_ref33","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.05.014"},{"key":"S0967199418000230_ref2","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12115"},{"key":"S0967199418000230_ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2010.09.005"},{"key":"S0967199418000230_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2014.04.013"},{"key":"S0967199418000230_ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.05.045"},{"key":"S0967199418000230_ref41","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(00)00099-3"},{"key":"S0967199418000230_ref3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2008.01240.x"},{"key":"S0967199418000230_ref9","first-page":"1","article-title":"Survival of collared peccaries (Pecari tajacu) sperm after freezing\u2013thawing using different extenders","volume":"42","author":"Campos","year":"2014","journal-title":"Acta Sci. Vet."},{"key":"S0967199418000230_ref34","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-736X2013000700014"},{"key":"S0967199418000230_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2015.05.033"},{"key":"S0967199418000230_ref6","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2001.tb03446.x"},{"key":"S0967199418000230_ref27","first-page":"253","article-title":"Developmental competence of dromedary camel oocytes fertilized in vitro by frozen\u2013thawed ejaculated and epididymal spermatozoa. Iran J.","volume":"17","author":"Scholkamy","year":"2016","journal-title":"Vet. Res."},{"key":"S0967199418000230_ref28","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12214"},{"key":"S0967199418000230_ref35","doi-asserted-by":"publisher","DOI":"10.1016\/j.repbio.2015.10.006"},{"key":"S0967199418000230_ref32","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.03.006"},{"key":"S0967199418000230_ref26","first-page":"787","article-title":"Superoxide dismutase and catalase activity in collared peccary (Pecari tajacu) seminal plasma and their relation to sperm quality","volume":"39","author":"Santos","year":"2018","journal-title":"Semina"},{"key":"S0967199418000230_ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.05.002"},{"key":"S0967199418000230_ref7","doi-asserted-by":"publisher","DOI":"10.2108\/zs130149"},{"key":"S0967199418000230_ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.08.002"},{"key":"S0967199418000230_ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.09.007"},{"key":"S0967199418000230_ref4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2010.01646.x"},{"key":"S0967199418000230_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.10.033"},{"key":"S0967199418000230_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2015.09.005"},{"key":"S0967199418000230_ref37","doi-asserted-by":"publisher","DOI":"10.1051\/rnd:19960506"},{"key":"S0967199418000230_ref39","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.09.044"},{"key":"S0967199418000230_ref23","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-09352011000600003"},{"key":"S0967199418000230_ref40","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.03.018"},{"key":"S0967199418000230_ref18","unstructured":"IUCN (2017) The IUCN Red List of Threatened Species. http:\/\/www.iucnredlist.org. Accessed 11 April 2017."},{"key":"S0967199418000230_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2017.01.008"},{"key":"S0967199418000230_ref24","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-09352013000400044"},{"key":"S0967199418000230_ref30","doi-asserted-by":"publisher","DOI":"10.1053\/tvjl.2002.0704"},{"key":"S0967199418000230_ref38","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00664-1"},{"key":"S0967199418000230_ref31","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2013.04.009"},{"key":"S0967199418000230_ref25","doi-asserted-by":"publisher","DOI":"10.1530\/REP-13-0220"},{"key":"S0967199418000230_ref36","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.01.007"},{"key":"S0967199418000230_ref17","doi-asserted-by":"publisher","DOI":"10.1530\/REP-15-0403"},{"key":"S0967199418000230_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2012.05.006"}],"container-title":["Zygote"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0967199418000230","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,13]],"date-time":"2019-04-13T18:30:24Z","timestamp":1555180224000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0967199418000230\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8]]},"references-count":41,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,8]]}},"alternative-id":["S0967199418000230"],"URL":"https:\/\/doi.org\/10.1017\/s0967199418000230","relation":{},"ISSN":["0967-1994","1469-8730"],"issn-type":[{"value":"0967-1994","type":"print"},{"value":"1469-8730","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8]]}}}