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At 18 h post injection, the oocyte was found to be fertilized with two clear pronuclei. The embryo divided and generated a four-cell embryo on day 2, which was replaced to the uterine cavity. Pregnancy test gave a positive \u03b2-human chorionic gonadotropin result, the scan performed at 7 weeks, revealed the presence of one amniotic sac with fetal heartbeat. Healthy live birth was obtained after 39 weeks of gestation.<\/jats:p>","DOI":"10.1017\/s0967199420000404","type":"journal-article","created":{"date-parts":[[2020,9,3]],"date-time":"2020-09-03T09:08:19Z","timestamp":1599124099000},"page":"516-518","source":"Crossref","is-referenced-by-count":0,"title":["Live birth following a single oocyte fertilized with ICSI and embryo transfer on day 2: a case report"],"prefix":"10.1017","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7698-8823","authenticated-orcid":false,"given":"Romualdo","family":"Sciorio","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisavet","family":"Angelaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naser","family":"Al-Azemi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2020,9,3]]},"reference":[{"key":"S0967199420000404_ref6","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00917"},{"key":"S0967199420000404_ref14","first-page":"1","article-title":"Embryo culture at a reduced oxygen concentration of 5%: a mini review","volume":"23","author":"Sciorio","year":"2019","journal-title":"Zygote"},{"key":"S0967199420000404_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2015.11.008"},{"key":"S0967199420000404_ref4","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00099"},{"key":"S0967199420000404_ref12","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(92)92425-F"},{"key":"S0967199420000404_ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2012.11.037"},{"key":"S0967199420000404_ref5","doi-asserted-by":"publisher","DOI":"10.23736\/S0031-0808.18.03507-3"},{"key":"S0967199420000404_ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2011.05.001"},{"key":"S0967199420000404_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(98)00024-7"},{"key":"S0967199420000404_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(78)92957-4"},{"key":"S0967199420000404_ref18","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1097\/GCO.0000000000000452","article-title":"What is new in the management of poor ovarian response in IVF?","volume":"30","author":"Vaiarelli","year":"2018","journal-title":"Curr Opin Obstet Gynecol"},{"key":"S0967199420000404_ref2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dey321"},{"key":"S0967199420000404_ref19","doi-asserted-by":"publisher","DOI":"10.1016\/S0070-2153(03)58003-6"},{"key":"S0967199420000404_ref17","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00094"},{"key":"S0967199420000404_ref10","unstructured":"Human Fertilisation and Embryology Authority (2018). 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