{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T16:04:13Z","timestamp":1791561853686,"version":"4.3.4"},"reference-count":25,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T00:00:00Z","timestamp":1598486400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec id=\"S0967199420000441_as1\"><jats:title>Purpose:<\/jats:title><jats:p>To report the utilization of diagnostic intracytoplasmic sperm injection (D-ICSI), an ICSI cycle performed in the natural cycle, to obtain information about embryo development potential after sperm injection into zona pellucida (ZP)-free oocytes.<\/jats:p><\/jats:sec><jats:sec id=\"S0967199420000441_as2\"><jats:title>Materials and Methods:<\/jats:title><jats:p>We report the case of a couple with primary unexplained infertility with a history of previous failed, <jats:italic>in vitro<\/jats:italic> fertilization intracytoplasmic sperm injection (IVF-ICSI) cycles characterized by the presence of ZP-free oocytes. Whole exome sequencing (WES) was carried out to analyse the possible genetic basis of oocyte abnormality.<\/jats:p><\/jats:sec><jats:sec id=\"S0967199420000441_as3\"><jats:title>Results:<\/jats:title><jats:p>Diagnostic ICSI provided information about the embryo development potential from ZP-free oocytes and allowed better planning of the subsequent ICSI cycle. WES revealed that the absence of ZP was likely to be due to a new (ZP1) mutation. The subsequent ICSI cycle resulted in the delivery of a healthy baby.<\/jats:p><\/jats:sec><jats:sec id=\"S0967199420000441_as4\"><jats:title>Discussion:<\/jats:title><jats:p>To the best of our knowledge, our report is the first to describe the use of D-ICSI to determine the feasibility of embryo development and implantation in a patient with ZP1 mutation, resulting in the subsequent delivery of a healthy baby. We used \u2018diagnostic\u2019 ICSI in the normal menstrual cycle to explore the feasibility of embryo development after sperm injection into ZP-free oocytes. Our results may expand the spectrum of diagnostic procedures associated with unexplained infertility.<\/jats:p><\/jats:sec>","DOI":"10.1017\/s0967199420000441","type":"journal-article","created":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T03:46:42Z","timestamp":1598500002000},"page":"519-523","source":"Crossref","is-referenced-by-count":16,"title":["Role of diagnostic intracytoplasmic sperm injection (ICSI) in the management of genetically determined zona pellucida-free oocytes during <i>in vitro<\/i> fertilization: a case report"],"prefix":"10.1017","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5751-9506","authenticated-orcid":false,"given":"Ahmad","family":"Metwalley","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nabeel","family":"Brasha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandro C.","family":"Esteves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Fawzy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hadiya","family":"Brasha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Hellani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manal","family":"El Hamshary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omaima","family":"Khamiss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2020,8,27]]},"reference":[{"key":"S0967199420000441_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)61187-X"},{"key":"S0967199420000441_ref16","unstructured":"World Health Organization (2010). 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