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This paper describes and evaluates critical factors affecting the success of oocyte vitrification. In particular, an appropriate oocyte stage such as maturation status (germinal vesicle stage, metaphase II stage), presence\/absence of cumulus cells before vitrification, and the effect of follicle size, as well as different culture systems and media for <jats:italic>in vitro<\/jats:italic> production of embryos, the types and concentrations of cryoprotectants, and cooling and warming rates at vitrification are considered. Special attention is paid to various cryocarriers used for low-volume vitrification, which ensures safe storage of oocytes\/embryos in liquid nitrogen and their successful post-thaw recovery. At the end, we focussed on how age of oocyte donors (heifers, cows) influences post-thaw development. This review summarizes results of recently published studies describing different methodologies of cryopreservation and post-thaw oocyte development with the main focus on vitrification of bovine oocytes.<\/jats:p>","DOI":"10.1017\/s0967199420000465","type":"journal-article","created":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T09:05:58Z","timestamp":1599210358000},"page":"1-11","source":"Crossref","is-referenced-by-count":27,"title":["Methodological approaches for vitrification of bovine oocytes"],"prefix":"10.1017","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8634-8432","authenticated-orcid":false,"given":"Linda","family":"Duj\u00ed\u010dkov\u00e1","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander 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