{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:49:44Z","timestamp":1761709784908,"version":"3.40.5"},"reference-count":37,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2020,11,25]],"date-time":"2020-11-25T00:00:00Z","timestamp":1606262400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The present study investigated the effects of c-type natriuretic peptide (CNP) on the development of murine preantral follicles during <jats:italic>in vitro<\/jats:italic> growth (IVG). Preantral follicles isolated from ovaries of Kunming mice were cultured <jats:italic>in vitro<\/jats:italic>. In the culture system, CNP was supplemented in the experimental groups and omitted in the control groups. In Experiment 1, CNP was only supplemented at the early stage and follicle development was evaluated. In Experiments 2 and 3, CNP was supplemented during the whole period of <jats:italic>in vitro<\/jats:italic> culture. In Experiment 2, follicle development and oocyte maturity were evaluated. In Experiment 3, follicle development and embryo cleavage after <jats:italic>in vitro<\/jats:italic> fertilization (IVF) were assessed. The results showed that in the control groups in all three experiments, granulosa cells migrated from within the follicle and the follicles could not reach the antral stage. In the experimental groups in all three experiments, no migration of granulosa cells was observed and follicle development was assessed as attaining the antral stage, which was significantly superior to that of the control group (<jats:italic>P<\/jats:italic> &lt; 0.0001). Oocyte meiotic arrest was effectively maintained, hence giving good developmental competence. In conclusion, CNP supplementation in the culture system during IVG benefited the development of murine preantral follicles.<\/jats:p>","DOI":"10.1017\/s096719942000060x","type":"journal-article","created":{"date-parts":[[2020,11,25]],"date-time":"2020-11-25T10:00:12Z","timestamp":1606298412000},"page":"150-154","source":"Crossref","is-referenced-by-count":5,"title":["Supplementation of c-type natriuretic peptide during <i>in vitro<\/i> growth period benefits the development of murine preantral follicles"],"prefix":"10.1017","volume":"29","author":[{"given":"Li","family":"Ang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cao","family":"Haixia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Hongxia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Ruijiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo","family":"Xingping","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8913-5689","authenticated-orcid":false,"given":"Wang","family":"Huaixiu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2020,11,25]]},"reference":[{"key":"S096719942000060X_ref27","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-015-1136-y"},{"key":"S096719942000060X_ref32","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2006.12.2739"},{"key":"S096719942000060X_ref37","first-page":"200","article-title":"Effects 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