{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T21:57:37Z","timestamp":1747173457762,"version":"3.40.5"},"reference-count":35,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T00:00:00Z","timestamp":1610668800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Knowledge of the sperm\u2013oocyte ratio in fish fertilization serves as the basis for studies on artificial reproduction and gamete manipulation. The aim of this study was to determine the minimum insemination dose for <jats:italic>Brycon amazonicus<\/jats:italic> oocyte fertilization. Female and male gametes were used and tested with the following doses of spermatozoa oocyte<jats:sup>\u20131<\/jats:sup> ml<jats:sup>\u20131<\/jats:sup>: 10,000, 20,000, 40,000, 60,000 and 80,000 (in triplicate). Fertilization rates were calculated and estimated from the regression equation by applying the segmented regression model \u2018Linear Response Plateau\u2019 to determine the appropriate proportion of gametes. Based on the equation \u0176 = 14.3415 + 0.0007836X, the fertilization rate increased up to 63.34% as it reached a plateau with a proportion of 62,524 spermatozoa oocyte<jats:sup>\u20131<\/jats:sup> ml<jats:sup>\u20131<\/jats:sup>, which is the minimum insemination dose recommended for artificial insemination of the species.<\/jats:p>","DOI":"10.1017\/s096719942000074x","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T04:53:22Z","timestamp":1610686402000},"page":"252-255","source":"Crossref","is-referenced-by-count":0,"title":["Inseminating dose for the artificial fertilization of <i>Brycon amazonicus<\/i> (Teleostei: Characidae)"],"prefix":"10.1017","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8777-1662","authenticated-orcid":false,"given":"Rosilane Gomes de Souza de","family":"Oliveira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marle Ang\u00e9lica","family":"Villacorta-Correa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2021,1,15]]},"reference":[{"key":"S096719942000074X_ref34","first-page":"655","article-title":"Priming hormone administration to induce spawning of some Brazilian migratory fish","volume":"56","author":"Zaniboni-Filho","year":"1996","journal-title":"Rev Bras Biol"},{"key":"S096719942000074X_ref8","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2109.1999.00326.x"},{"key":"S096719942000074X_ref24","first-page":"139","article-title":"Sele\u00e7\u00e3o e caracteriza\u00e7\u00e3o de f\u00eameas de matrinx\u00e3, Brycon cephalus, induzidas a reprodu\u00e7\u00e3o","volume":"27","author":"Romagosa","year":"2001","journal-title":"Bol Inst Pesca"},{"key":"S096719942000074X_ref29","doi-asserted-by":"publisher","DOI":"10.1016\/0044-8486(94)00395-5"},{"key":"S096719942000074X_ref35","first-page":"41","article-title":"Caracteriza\u00e7\u00e3o morfol\u00f3gica da matrinx\u00e3, Brycon cephalus (G\u00fcnther, 1869) (Teleostei: Characidae)","volume":"48","author":"Zaniboni-Filho","year":"1988","journal-title":"Revista Brasileira Biologia"},{"key":"S096719942000074X_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2019.05.004"},{"key":"S096719942000074X_ref18","doi-asserted-by":"publisher","DOI":"10.1016\/0044-8486(88)90030-0"},{"key":"S096719942000074X_ref23","doi-asserted-by":"publisher","DOI":"10.1054\/tice.1999.0064"},{"key":"S096719942000074X_ref27","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-09352007000400009"},{"key":"S096719942000074X_ref9","first-page":"104","volume-title":"Manual para exames androl\u00f3gicos e avalia\u00e7\u00e3o de s\u00eamen animal","author":"Col\u00e9gio Brasileiro de Reprodu\u00e7\u00e3o","year":"2013"},{"key":"S096719942000074X_ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2003.12.006"},{"key":"S096719942000074X_ref2","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199415000210"},{"key":"S096719942000074X_ref17","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-09352013000200018"},{"key":"S096719942000074X_ref10","first-page":"152","article-title":"Evaluaci\u00f3n de diferentes crioprotectores para la crioconservaci\u00f3n de espermatozoides de yam\u00fa (Brycon amazonicus)","volume":"19","author":"Cruz-Casallas","year":"2006","journal-title":"Rev Colomb Cienc Pec"},{"key":"S096719942000074X_ref33","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2009.02.021"},{"key":"S096719942000074X_ref21","first-page":"19","article-title":"Inducci\u00f3n de la ovulaci\u00f3n y del desove del yam\u00fa, Brycon siebenthale, con implantes de mGnRH-a","volume":"28","author":"Pardo-Carrasco","year":"2002","journal-title":"Bol Inst Pesca"},{"key":"S096719942000074X_ref7","doi-asserted-by":"publisher","DOI":"10.1590\/S1516-35982006000500001"},{"key":"S096719942000074X_ref12","first-page":"59","volume-title":"Cryopreservation in aquatic species","author":"Denniston","year":"2000"},{"key":"S096719942000074X_ref15","unstructured":"Honczaryk, A and Inoue, LAKA (2009). 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Embrapa Amaz\u00f4nia Ocidental-Circular T\u00e9cnica (INFOTECA-E)."},{"key":"S096719942000074X_ref13","first-page":"44","article-title":"Spermatozoal traits and sperm competition in Atlantic salmon: relative sperm velocity is the primary determinant of fertilization success","volume":"14","author":"Gage","year":"2004","journal-title":"Curr Biol"},{"key":"S096719942000074X_ref22","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199417000624"},{"key":"S096719942000074X_ref31","first-page":"335","article-title":"Eugenol como anest\u00e9sico para juvenis de matrinx\u00e3 (\u2018Brycon cephalus\u2019)","volume":"8","author":"Vidal","year":"2007","journal-title":"Rev Bras Sa\u00fad Prod Anim"},{"key":"S096719942000074X_ref5","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402610203"},{"key":"S096719942000074X_ref14","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-09352012000200038"},{"key":"S096719942000074X_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/0044-8486(94)00231-C"},{"key":"S096719942000074X_ref20","doi-asserted-by":"publisher","DOI":"10.1590\/S1516-89132006000500015"},{"key":"S096719942000074X_ref11","unstructured":"Cruz-Casallas, PE and Velasco-Santamar\u00eda, YM (2004). 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