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However, the development of this technique depends on steps that include ovarian stimulation, collection and oocyte maturation. The purpose of this study was to assess the feasibility of an ovarian stimulation protocol for follicular aspiration, the efficiency of videolaparoscopy for follicular aspiration and test a medium for <jats:italic>in vitro<\/jats:italic> oocyte maturation for the species <jats:italic>Mazama gouazoubira<\/jats:italic>. Five females were submitted to repeated ovarian stimulation (hormone protocol using controlled internal drug release), and estradiol benzoate on D0 and eight injections of follicle-stimulating hormone, once every 12 h, from D4 onwards at 30-day intervals. Fourteen surgical procedures were performed in superstimulated females, resulting in the collection of 94 oocytes and an average of 17.1 \u00b1 9.1 follicles observed, 13.5 \u00b1 6.6 follicles aspirated and 7.2 \u00b1 3.7 oocytes collected per surgery. After collection, the oocytes were submitted to <jats:italic>in vitro<\/jats:italic> maturation for 24 h and stained with Hoechst 33342 dye to evaluate their nuclear status; 64.5% of the oocytes reached MII and 16.1% were spontaneously activated by parthenogenesis. The nuclear status of oocytes that did not undergo <jats:italic>in vitro<\/jats:italic> maturation was evaluated; 80.9% were found to be immature.<\/jats:p>","DOI":"10.1017\/s0967199420000787","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T04:53:21Z","timestamp":1610686401000},"page":"216-222","source":"Crossref","is-referenced-by-count":5,"title":["Collection and <i>in vitro<\/i> maturation of <i>Mazama gouazoubira<\/i> (brown brocket deer) oocytes obtained after ovarian stimulation"],"prefix":"10.1017","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3652-1011","authenticated-orcid":false,"given":"Luciana Diniz","family":"Rola","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eveline dos Santos","family":"Zanetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maite","family":"del Collado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen de F\u00e1tima Carvalho","family":"Peroni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Maur\u00edcio Barbanti","family":"Duarte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2021,1,15]]},"reference":[{"key":"S0967199420000787_ref10","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(00)00065-8"},{"key":"S0967199420000787_ref25","first-page":"8","volume-title":"Guia de identifica\u00e7\u00e3o de cerv\u00eddeos brasileiros","author":"Duarte","year":"1996"},{"key":"S0967199420000787_ref35","first-page":"669","article-title":"Antigonadotropins in the serum of cows following repeated therapeutic pregnant mare serum injections","volume":"27","author":"Jainudeen","year":"1966","journal-title":"Am J Vet Res"},{"key":"S0967199420000787_ref44","doi-asserted-by":"publisher","DOI":"10.1530\/acta.0.XXXV0253"},{"key":"S0967199420000787_ref3","unstructured":"Abreu, CO (2006). Efeito da vitamina E na criopreserva\u00e7\u00e3o de s\u00eamen de veado-boror\u00f3-do-sul (Mazama nana). [Doctoral dissertation.] Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias, Universidade Estadual Paulista, Jaboticabal, S\u00e3o Paulo, p. 57."},{"key":"S0967199420000787_ref48","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00480-0"},{"key":"S0967199420000787_ref12","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod54.2.347"},{"key":"S0967199420000787_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.04.052"},{"key":"S0967199420000787_ref49","unstructured":"Siriaroonrat, B (2006). Development of in vitro fertilization for the endangered Eld\u00b4s deer (Cervus eldi thamin): Effects of in vitro maturation, fertilization and culture on developmental competence. 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