{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:11:40Z","timestamp":1790691100474,"version":"4.1.0"},"reference-count":43,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T00:00:00Z","timestamp":1661904000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Domestic cat embryos generated by<jats:italic>in vitro<\/jats:italic>fertilization (IVF) and cultured without the zona pellucida have a reduced implantation capacity after embryo transfer at the blastocyst stage. The objective of this study was to evaluate the expression of trophectoderm markers in domestic cat blastocysts cultured without the zona pellucida. Two experimental groups were selected: (1) domestic cat embryos generated by IVF and cultured<jats:italic>in vitro<\/jats:italic>normally (zona intact group, ZI); and (2) domestic cat embryos generated by IVF and cultured<jats:italic>in vitro<\/jats:italic>without a zona pellucida (zona-free group, ZF). In the ZF group, the zona pellucida of the presumptive zygote was removed and these were cultured using the well of the well (WOW) system.<jats:italic>In vitro<\/jats:italic>culture was carried out for 7 days. The cleavage, morula and blastocyst rates were estimated. Finally, the relative expression levels of the trophectoderm markers<jats:italic>TEAD4<\/jats:italic>,<jats:italic>YAP1<\/jats:italic>,<jats:italic>CDX2<\/jats:italic>and<jats:italic>EOMES<\/jats:italic>, the cell adhesion marker<jats:italic>E-cadherin<\/jats:italic>and the apoptosis marker<jats:italic>CASP3<\/jats:italic>were evaluated by RT-qPCR in the blastocysts. The Wilcoxon test was used to evaluate differences (<jats:italic>P<\/jats:italic>&lt; 0.05). No differences were observed in the cleavage, morula and blastocyst rates between the ZF and ZI groups. No differences were found in the expression of<jats:italic>TEAD4<\/jats:italic>,<jats:italic>CDX2<\/jats:italic>,<jats:italic>E-cadherin<\/jats:italic>and<jats:italic>CASP3<\/jats:italic>between groups. The expression of<jats:italic>YAP1<\/jats:italic>and<jats:italic>EOMES<\/jats:italic>was higher in ZF blastocysts than in ZI blastocysts. In conclusion, the<jats:italic>in vitro<\/jats:italic>culture without the zona pellucida generates an overexpression of<jats:italic>YAP1<\/jats:italic>and<jats:italic>EOMES<\/jats:italic>in the domestic cat blastocysts. More studies are needed to confirm if this overexpression might affect<jats:italic>in vivo<\/jats:italic>development.<\/jats:p>","DOI":"10.1017\/s096719942200034x","type":"journal-article","created":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T08:08:49Z","timestamp":1661933329000},"page":"841-848","source":"Crossref","is-referenced-by-count":4,"title":["Analysis of trophectoderm markers in domestic cat blastocysts cultured without zona pellucida"],"prefix":"10.1017","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3401-1237","authenticated-orcid":false,"given":"Daniel","family":"Veraguas-D\u00e1vila","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darling","family":"Sa\u00e9z-Ru\u00edz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Consuelo","family":"\u00c1lvarez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Saravia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fidel Ovidio","family":"Castro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lleretny","family":"Rodr\u00edguez-Alvarez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2022,8,31]]},"reference":[{"key":"S096719942200034X_ref26","doi-asserted-by":"publisher","DOI":"10.1089\/153623003321512111"},{"key":"S096719942200034X_ref12","doi-asserted-by":"publisher","DOI":"10.1071\/RD08222"},{"key":"S096719942200034X_ref29","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.12.018"},{"key":"S096719942200034X_ref39","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2020.02.012"},{"key":"S096719942200034X_ref43","doi-asserted-by":"publisher","DOI":"10.1242\/dev.056630"},{"key":"S096719942200034X_ref7","article-title":"TEAD4, YAP1 and WWTR1 prevent the premature onset of pluripotency prior to the 16-cell stage","volume":"146","author":"Frum","year":"2019","journal-title":"Development"},{"key":"S096719942200034X_ref21","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-30406-4_9"},{"key":"S096719942200034X_ref19","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.40167"},{"key":"S096719942200034X_ref2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg507"},{"key":"S096719942200034X_ref16","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.57.641"},{"key":"S096719942200034X_ref30","doi-asserted-by":"publisher","DOI":"10.1038\/35003601"},{"key":"S096719942200034X_ref3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0146390"},{"key":"S096719942200034X_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)53460-8"},{"key":"S096719942200034X_ref8","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.112.098855"},{"key":"S096719942200034X_ref9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0110998"},{"key":"S096719942200034X_ref37","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13229"},{"key":"S096719942200034X_ref41","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-010X(19991015)285:3<251::AID-JEZ8>3.0.CO;2-1"},{"key":"S096719942200034X_ref11","doi-asserted-by":"publisher","DOI":"10.1089\/clo.2008.0021"},{"key":"S096719942200034X_ref13","doi-asserted-by":"publisher","DOI":"10.1089\/cell.2010.0111"},{"key":"S096719942200034X_ref15","unstructured":"International Union for Conservation of Nature and Natural Resources. 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