{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T05:30:45Z","timestamp":1783488645245,"version":"3.55.0"},"reference-count":45,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T00:00:00Z","timestamp":1676851200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Carvacrol (C<jats:sub>10<\/jats:sub>H<jats:sub>14<\/jats:sub>O), an efficient phenolic antioxidant substance for several cell types, may become a useful antioxidant for female germ cells and embryo culture. This study investigates the effects of carvacrol supplementation on bovine oocytes in <jats:italic>in vitro<\/jats:italic> maturation (IVM) and embryo production. In total, 1222 cumulus\u2013oocyte complexes were cultured in TCM-199<jats:sup>+<\/jats:sup> alone (control treatment) or supplemented with carvacrol at the concentrations of 3 \u00b5M (Carv-3), 12.5 \u00b5M (Carv-12.5), or 25 \u00b5M (Carv-25). After IVM, the oocytes were subjected to <jats:italic>in vitro<\/jats:italic> fertilization and embryo production, and the spent medium post-IVM was used for evaluating the levels of reactive oxygen species and the antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate and 2,2\u2032-azinobis-3-ethyl-benzothiozoline-6-sulphonic acid quantification). A greater (<jats:italic>P<\/jats:italic> &lt; 0.05) antioxidant potential was observed in the spent medium of all carvacrol-treated groups compared with the control medium. Moreover, the addition of carvacrol to the maturation medium did not affect (<jats:italic>P<\/jats:italic> &gt; 0.05) blastocyst production on days 7 and 10 of culture; however, the total number of cells per blastocyst was reduced (<jats:italic>P<\/jats:italic> &lt; 0.05) in two carvacrol-treated groups (Carv-3 and Carv-25). In conclusion, carvacrol demonstrated a high antioxidant capacity in the spent medium after oocyte maturation; however, although embryo production was not affected, in general, carvacrol addition to IVM medium reduced the total number of cells per blastocyst. Therefore, due to the high antioxidant capacity of carvacrol, new experiments are warranted to investigate the beneficial effects of lower concentrations of carvacrol on embryo production in cattle and other species.<\/jats:p>","DOI":"10.1017\/s0967199422000673","type":"journal-article","created":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T09:56:35Z","timestamp":1676886995000},"page":"173-179","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":20,"title":["Effect of carvacrol antioxidant capacity on oocyte maturation and embryo production in 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Published by Cambridge University Press","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http:\/\/creativecommons.org\/licenses\/by\/4.0\/), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}