{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T14:48:41Z","timestamp":1790952521775,"version":"4.1.0"},"reference-count":58,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2023,2,27]],"date-time":"2023-02-27T00:00:00Z","timestamp":1677456000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Regarding the low number of embryos that reach the blastocyst stage when cultured <jats:italic>in vitro<\/jats:italic>, this study aimed to evaluate the effects of quercetin on pre-implantation mouse (<jats:italic>Mus musculus<\/jats:italic>) embryos obtained using <jats:italic>in vitro<\/jats:italic> fertilization, especially during the passage from morula to blastocyst. Furthermore, we studied whether quercetin also affected the expression of hypoxia-inducible factor 1\u03b1 (HIF-1\u03b1). The culture medium for the embryos was supplemented with quercetin, for long or short periods of time, and then the development potential, total cell number, apoptosis rates and expression of HIF-1\u03b1 were studied to determine the effect of quercetin. Embryos failed to develop when cultured for long periods of time with quercetin, implying the possible toxic effects of this, alternatively antioxidant, compound. However, a short culture from morula to blastocyst significantly improved the development potential of <jats:italic>in vitro<\/jats:italic> produced embryos, increasing the final total cell number and reducing the apoptosis rate, observing similar results to those embryos cultured in low-oxygen concentrations or developed <jats:italic>in utero<\/jats:italic>. Furthermore, in embryos treated with quercetin for 2 or 4 h we found an increase in HIF-1\u03b1 compared with untreated embryos. This work could imply a way to use quercetin in fertility clinics to improve the production of healthy blastocysts and, consequently, increase the success rates in assisted reproduction techniques.<\/jats:p>","DOI":"10.1017\/s0967199423000060","type":"journal-article","created":{"date-parts":[[2023,2,27]],"date-time":"2023-02-27T01:47:11Z","timestamp":1677462431000},"page":"225-236","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":3,"title":["Supplementation of culture medium with quercetin improves mouse blastocyst quality and increases the expression of HIF-1\u03b1 protein"],"prefix":"10.1017","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6812-1189","authenticated-orcid":false,"given":"Nuria","family":"Hern\u00e1ndez-Roll\u00e1n","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soledad","family":"S\u00e1nchez-Mateos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"L\u00f3pez-Morat\u00f3","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco M.","family":"S\u00e1nchez-Margallo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio S.","family":"\u00c1lvarez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2023,2,27]]},"reference":[{"key":"S0967199423000060_ref18","doi-asserted-by":"publisher","DOI":"10.1080\/10715760290025951"},{"key":"S0967199423000060_ref32","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)60312-4"},{"key":"S0967199423000060_ref41","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-8-83"},{"key":"S0967199423000060_ref49","doi-asserted-by":"publisher","DOI":"10.1038\/229132a0"},{"key":"S0967199423000060_ref54","first-page":"29","volume-title":"Abstracts of the 2nd Annual Meeting","author":"Whitten","year":"1969"},{"key":"S0967199423000060_ref14","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0990673"},{"key":"S0967199423000060_ref16","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/7.2.175"},{"key":"S0967199423000060_ref24","first-page":"e17834","article-title":"Quercetin preventive effects on theophylline-induced anomalies in rat embryo","volume":"9","author":"Karampour","year":"2014","journal-title":"Jundishapur Journal of Natural Pharmaceutical Products"},{"key":"S0967199423000060_ref31","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dms055"},{"key":"S0967199423000060_ref8","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-2519"},{"key":"S0967199423000060_ref58","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0089520"},{"key":"S0967199423000060_ref56","doi-asserted-by":"publisher","DOI":"10.1111\/j.1753-4887.2008.00059.x"},{"key":"S0967199423000060_ref6","first-page":"155","article-title":"Response to hypoxia. 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