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The current study aimed to investigate the effect of<jats:italic>Stevia rebaudiana<\/jats:italic>hydroalcoholic extract on serum testosterone levels, sperm parameters,<jats:italic>in vitro<\/jats:italic>fertilization (IVF) success, and<jats:italic>in vitro<\/jats:italic>embryonic developmental potential to reach the blastocyst stage in a streptozotocin (STZ)-induced mouse model of diabetes. In this research, 30 male mice were distributed randomly into control, diabetic (streptozotocin 150 mg\/kg) and diabetic +<jats:italic>Stevia<\/jats:italic>(400 mg\/kg) groups. The results revealed a decrease in body and testis weight and elevated blood fasting blood sugar (FBS) levels in the diabetic group, compared with the control. However,<jats:italic>Stevia<\/jats:italic>treatment significantly increased body and testis weight, while serum FBS levels were decreased compared with the diabetic group. In addition,<jats:italic>Stevia<\/jats:italic>significantly increased blood testosterone levels compared with the diabetic group. Moreover, sperm parameters were improved considerably by<jats:italic>Stevia<\/jats:italic>treatment compared with the diabetic group. Furthermore,<jats:italic>Stevia<\/jats:italic>administration significantly promoted IVF success rate and<jats:italic>in vitro<\/jats:italic>development of fertilized oocytes compared with the diabetic group. In summary, our data indicated that<jats:italic>Stevia<\/jats:italic>enhanced sperm parameters, IVF success, and<jats:italic>in vitro<\/jats:italic>embryonic developmental competency in diabetic mice, probably because of its antioxidant effects. Therefore,<jats:italic>Stevia<\/jats:italic>could ameliorate sperm parameters that, in turn, increase fertilization outcomes in experimental-induced diabetes.<\/jats:p>","DOI":"10.1017\/s0967199423000266","type":"journal-article","created":{"date-parts":[[2023,7,7]],"date-time":"2023-07-07T07:17:36Z","timestamp":1688714256000},"page":"475-482","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":6,"title":["Ameliorative effect of<i>Stevia rebaudiana<\/i>Bertoni on sperm parameters,<i>in vitro<\/i>fertilization, and early embryo development in a streptozotocin-induced mouse model of diabetes"],"prefix":"10.1017","volume":"31","author":[{"given":"Mahdad","family":"Abdi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fathemeh","family":"Alizadeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3427-5478","authenticated-orcid":false,"given":"Erfan","family":"Daneshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morteza","family":"Abouzaripour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fardin","family":"Fathi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8674-3993","authenticated-orcid":false,"given":"Kaveh","family":"Rahimi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2023,7,7]]},"reference":[{"key":"S0967199423000266_ref11","first-page":"299","article-title":"Stevia aquatic extract protects the pancreas from streptozocin (STZ) induced damage: A stereological study","volume":"60","author":"Dastghaib","year":"2022","journal-title":"Indian Journal of Experimental Biology"},{"key":"S0967199423000266_ref22","first-page":"193","article-title":"Diagnosis of diabetes","volume":"368","author":"Inzucchi","year":"2013","journal-title":"New England Journal of Medicine"},{"key":"S0967199423000266_ref13","doi-asserted-by":"publisher","DOI":"10.1002\/0471141755.ph0547s70"},{"key":"S0967199423000266_ref21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.37962\/jbas.v12i1.344","article-title":"Therapeutic attributes of Stevia rebaudiana Leaves in diabetic animal model","volume":"12","author":"Hussain","year":"2021","journal-title":"RADS Journal of Biological Research and Applied Science"},{"key":"S0967199423000266_ref1","doi-asserted-by":"publisher","DOI":"10.5620\/eaht.2021013"},{"key":"S0967199423000266_ref6","first-page":"65","article-title":"Hypoglycemic effect of aquatic extract of Stevia in pancreas of diabetic rats: PPAR\u03b3-dependent regulation or antioxidant potential","volume":"8","author":"Assaei","year":"2016","journal-title":"Avicenna Journal of Medical Biotechnology"},{"key":"S0967199423000266_ref15","doi-asserted-by":"publisher","DOI":"10.5812\/semj.61044"},{"key":"S0967199423000266_ref19","doi-asserted-by":"publisher","DOI":"10.1166\/asl.2018.12110"},{"key":"S0967199423000266_ref31","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmx033"},{"key":"S0967199423000266_ref38","doi-asserted-by":"publisher","DOI":"10.18203\/2320-1770.ijrcog20181892"},{"key":"S0967199423000266_ref41","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)43019-5"},{"key":"S0967199423000266_ref43","doi-asserted-by":"publisher","DOI":"10.15344\/2456-8007\/2020\/142"},{"key":"S0967199423000266_ref45","doi-asserted-by":"publisher","DOI":"10.1201\/9780429244216-5"},{"key":"S0967199423000266_ref20","doi-asserted-by":"publisher","DOI":"10.21873\/invivo.12531"},{"key":"S0967199423000266_ref26","doi-asserted-by":"publisher","DOI":"10.2147\/JEP.S249964"},{"key":"S0967199423000266_ref3","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.451773"},{"key":"S0967199423000266_ref18","doi-asserted-by":"publisher","DOI":"10.4158\/EP09355.OR"},{"key":"S0967199423000266_ref29","doi-asserted-by":"publisher","DOI":"10.1007\/s42399-018-0012-9"},{"key":"S0967199423000266_ref14","doi-asserted-by":"publisher","DOI":"10.1111\/and.13469"},{"key":"S0967199423000266_ref30","first-page":"267","article-title":"Stevia rebaudiana (Bert.) 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