{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T03:20:28Z","timestamp":1767151228914,"version":"build-2238731810"},"reference-count":56,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T00:00:00Z","timestamp":1703635200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Mouse testicular tissue is composed of seminiferous tubules and interstitial tissue. Mammalian spermatogenesis is divided into three stages: spermatocytogenesis (mitotic divisions) in which spermatogonial stem cells (SSCs) turn into spermatocytes, followed by two consecutive meiotic divisions in which spermatocytes form spermatids. Spermatids differentiate into spermatozoa during spermiogenesis. Various factors affect the process of spermatogenesis and the organization of cells in the testis. Any disorder in different stages of spermatogenesis will have negative effects on male fertility. The aim of the current study was to compare the\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    spermatogenesis processes before and after transplantation to azoospermic mice using ultrastructural techniques. In this study, mice were irradiated with single doses of 14 Gy\n                    <jats:sup>60<\/jats:sup>\n                    Co radiation. SSCs isolated from neonatal mice were cultured\n                    <jats:italic>in vitro<\/jats:italic>\n                    for 1 week and were injected into the seminiferous tubule recipient\u2019s mice. Testicular cells of neonatal mice were cultured in the four groups on extracellular matrix-based 3D printing scaffolds. The transplanted testes (8 weeks after transplantation) and cultured testicular cells\n                    <jats:italic>in vitro<\/jats:italic>\n                    (after 3 weeks) were then processed for transmission electron microscopy studies. Our study\u2019s findings revealed that the morphology and ultrastructure of testicular cells after transplantation and\n                    <jats:italic>in vitro<\/jats:italic>\n                    culture are similar to those of\n                    <jats:italic>in vivo<\/jats:italic>\n                    spermatogenesis, indicating that spermatogenic cell nature is unaltered\n                    <jats:italic>in vitro<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1017\/s096719942300062x","type":"journal-article","created":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T04:21:35Z","timestamp":1703650895000},"page":"87-95","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":3,"title":["RETRACTED \u2013 Ultrastructural study:\n                    <i>in vitro<\/i>\n                    and\n                    <i>in vivo<\/i>\n                    differentiation of mice spermatogonial stem"],"prefix":"10.1017","volume":"32","author":[{"given":"Zahra","family":"Bashiri","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mansoureh","family":"Movahedin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vahid","family":"Pirhajati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hamidreza","family":"Asgari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2661-0117","authenticated-orcid":false,"given":"Morteza","family":"Koruji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2023,12,27]]},"reference":[{"key":"S096719942300062X_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2022.07.127"},{"key":"S096719942300062X_ref52","first-page":"117","article-title":"Spermatozoal morphology in the \u201csymmetrical\u201d hermit crab. 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