{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T18:39:18Z","timestamp":1785523158085,"version":"3.56.0"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T00:00:00Z","timestamp":1726704000000},"content-version":"unspecified","delay-in-days":49,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The aims of this study were to evaluate the doxorubicin concentration that induces toxic effects on <jats:italic>in vitro<\/jats:italic> culture of isolated mouse secondary follicles and to investigate whether resveratrol can inhibit or reduce this toxicity. Secondary follicles were isolated and cultured for 12 days in control medium (\u03b1-MEM<jats:sup>+<\/jats:sup>) or in \u03b1-MEM<jats:sup>+<\/jats:sup> supplemented with doxorubicin (0.1 \u00b5g\/ml) or different concentrations of resveratrol (0.5, 2, or 5 \u00b5M) associated with doxorubicin (0.1 \u00b5g\/ml) (experiment 1). For experiment 2, follicles were cultured in \u03b1-MEM<jats:sup>+<\/jats:sup> alone or supplemented with doxorubicin (0.3 \u00b5g\/ml) or different concentrations of resveratrol (5 or 10 \u00b5M) associated or not with doxorubicin (0.3 \u00b5g\/ml) (experiment 2). The endpoints analyzed were morphology (survival), antrum formation, follicular diameter, mitochondrial activity, glutathione (GSH) levels and DNA fragmentation. In the first experiment, doxorubicin (0.1 \u00b5g\/ml) maintained survival and antrum formation similar to the control, while 5 \u00b5M resveratrol showed increased parameters, maintained mitochondrial activity and increased GSH levels compared to the control. In the second experiment, doxorubicin (0.3 \u00b5g\/ml) reduced survival, antrum formation and follicular diameter compared to the control. Resveratrol at a concentration of 10 \u00b5M attenuated the damage caused by doxorubicin by improving follicular survival and did not present DNA fragmentation. In conclusion, supplementation of the <jats:italic>in vitro<\/jats:italic> culture medium with 0.3 \u00b5g\/ml doxorubicin reduced the survival and impaired the development of mouse-isolated preantral follicles. Resveratrol at 10 \u00b5M reduced doxorubicin-induced follicular atresia, without DNA fragmentation in the follicles.<\/jats:p>","DOI":"10.1017\/s0967199424000315","type":"journal-article","created":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T08:55:28Z","timestamp":1726736128000},"page":"294-302","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":5,"title":["Resveratrol attenuates doxorubicin-induced toxicity during <i>in vitro<\/i> culture of mouse-isolated preantral follicles"],"prefix":"10.1017","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7954-573X","authenticated-orcid":false,"given":"G.A.L.","family":"Silva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.P.O","family":"Monte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.T.V.","family":"Fran\u00e7a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.M.","family":"Mota","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.L.","family":"Oliveira Junior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.O.","family":"Andrade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.M.","family":"Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.L.S.","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.S.","family":"Guimar\u00e3es","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.S.","family":"Barberino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.C.","family":"Palheta Junior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.E.J.","family":"Smitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6215-5292","authenticated-orcid":false,"given":"M.H.T.","family":"Matos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2024,9,19]]},"container-title":["Zygote"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0967199424000315","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T06:25:20Z","timestamp":1752560720000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0967199424000315\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["S0967199424000315"],"URL":"https:\/\/doi.org\/10.1017\/s0967199424000315","relation":{},"ISSN":["0967-1994","1469-8730"],"issn-type":[{"value":"0967-1994","type":"print"},{"value":"1469-8730","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8]]},"assertion":[{"value":"\u00a9 The Author(s), 2024. Published by Cambridge University Press","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}