{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T03:43:22Z","timestamp":1779335002797,"version":"3.51.4"},"reference-count":26,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T00:00:00Z","timestamp":1730246400000},"content-version":"unspecified","delay-in-days":29,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) has been shown to play a significant role in oxidative stress across various tissues and cells; however, its role in sperm function remains poorly understood. This study aimed to investigate the protective effect of GYY4137, a slow-releasing H<jats:sub>2<\/jats:sub>S compound, on sperm damage induced by H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>. We assessed the effects of GYY4137 on motility, viability, lipid peroxidation and caspase-3 activity in human spermatozoa in vitro following oxidative damage mediated by H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>. Spermatozoa from 25 healthy men were selected using a density gradient centrifugation method and cultured in the presence or absence of 10 \u03bcM H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, followed by incubation with varying concentrations of GYY4137 (0.625\u20132.5 \u03bcM). After 24 h of incubation, sperm motility, viability, lipid peroxidation, and caspase-3 activity were evaluated. The results indicated that H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> adversely affected sperm parameters, reducing motility and viability, while increasing oxidative stress, as evidenced by elevated lipid peroxidation and caspase-3 activity. GYY4137 provided dose-dependent protection against H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>-induced oxidative stress (OS). We concluded that supplementation with GYY4137 may offer antioxidant protection during in vitro sperm preparation for assisted reproductive technology.<\/jats:p>","DOI":"10.1017\/s0967199424000340","type":"journal-article","created":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T09:57:28Z","timestamp":1730282248000},"page":"360-365","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":4,"title":["Treatment of human sperm with GYY4137 increases sperm motility and resistance to oxidative 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