{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T08:18:18Z","timestamp":1762244298473,"version":"build-2065373602"},"reference-count":52,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T00:00:00Z","timestamp":1748908800000},"content-version":"unspecified","delay-in-days":2,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    This study aimed to evaluate the effects of acetyl-L-carnitine on follicle survival and growth, stromal cell density and extracellular matrix, as well as on the expression of mRNA for nuclear factor erythroid 2-related factor (\n                    <jats:italic>NRF2<\/jats:italic>\n                    ), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and peroxiredoxin 6 (PRDX6) in cultured bovine ovarian cortical tissues. Ovarian fragments (3 \u00d7 3 \u00d7 1 mm) were cultured for 6 days in \u03b1-MEM\n                    <jats:sup>+<\/jats:sup>\n                    alone or supplemented with 10, 50 or 100 \u03bcM acetyl-L-carnitine at 38.5\u00b0C with 5% CO\n                    <jats:sub>2<\/jats:sub>\n                    in humidified air. Before (non-cultured tissues) and after culture, the ovarian fragments were fixed in 4% paraformaldehyde for 12 h for histological analysis or stored at \u201380\u00baC for mRNA expression analysis of\n                    <jats:italic>NRF2, SOD, CAT, PRDX6<\/jats:italic>\n                    and\n                    <jats:italic>GPX1<\/jats:italic>\n                    . The results showed that 100 \u03bcM acetyl-L-carnitine increased the percentages of morphologically normal follicles and stromal cell density in cultured ovarian tissues. On the other hand, acetyl-L-carnitine did not influence the percentage of collagen in ovarian tissue nor the expression of mRNAs for\n                    <jats:italic>NRF2, SOD, CAT, PRDX6<\/jats:italic>\n                    and\n                    <jats:italic>GPX1<\/jats:italic>\n                    . In conclusion, 100 \u03bcM acetyl-L-carnitine increased follicle survival and stromal cell density in cultured bovine ovarian tissues but does not influence collagen fibre distribution or the expression of mRNAs for\n                    <jats:italic>NRF2, SOD, CAT, PRDX6<\/jats:italic>\n                    and\n                    <jats:italic>GPX1<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1017\/s0967199425000103","type":"journal-article","created":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T03:03:57Z","timestamp":1748919837000},"page":"125-131","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":0,"title":["Acetyl-L-carnitine increases follicle survival and stromal cell density in cultured bovine ovarian tissues"],"prefix":"10.1017","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2404-5524","authenticated-orcid":false,"given":"Allana M.F.","family":"Leit\u00e3o","sequence":"first","affiliation":[{"name":"Federal University of Ceara"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonia V.N.","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Federal University of Ceara"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bianca R.","family":"Silva","sequence":"additional","affiliation":[{"name":"Federal University of Ceara"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Efig\u00eania C.","family":"Barbalho","sequence":"additional","affiliation":[{"name":"Federal University of Ceara"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ernando I.","family":"Teixeira de Assis","sequence":"additional","affiliation":[{"name":"Federal University of Ceara"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7045-1523","authenticated-orcid":false,"given":"Francisco C.","family":"Costa","sequence":"additional","affiliation":[{"name":"Federal University of Ceara"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabricio S.","family":"Martins","sequence":"additional","affiliation":[{"name":"State University of Valley Acara\u00fa (UVA)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5970-6177","authenticated-orcid":false,"given":"Jos\u00e9 R.V.","family":"Silva","sequence":"additional","affiliation":[{"name":"Federal University of Ceara"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2025,6,3]]},"reference":[{"key":"S0967199425000103_ref9","doi-asserted-by":"publisher","DOI":"10.1111\/anu.13093"},{"key":"S0967199425000103_ref12","first-page":"248","article-title":"Tecnologia do ov\u00e1rio artificial: aplica\u00e7\u00f5es, estado da arte, limita\u00e7\u00f5es e perspectivas","volume":"41","author":"De Figueiredo","year":"2017","journal-title":"Revista Brasileira de Reprodu\u00e7\u00e3o Animal"},{"key":"S0967199425000103_ref45","doi-asserted-by":"publisher","DOI":"10.1007\/s12325-021-01789-5"},{"key":"S0967199425000103_ref5","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11061054"},{"key":"S0967199425000103_ref49","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-14936-3"},{"key":"S0967199425000103_ref7","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199418000497"},{"key":"S0967199425000103_ref43","first-page":"166","article-title":"Regula\u00e7\u00e3o enzim\u00e1tica e metab\u00f3lica da succinila\u00e7\u00e3o da lisina","volume":"7","author":"Sreedhar","year":"2020","journal-title":"Genes and Doen\u00e7as"},{"key":"S0967199425000103_ref50","first-page":"e920250","article-title":"Anti-inflammatory and antioxidant effects of acetyl-l-carnitine in atherosclerotic rats","volume":"26","author":"Wang","year":"2020","journal-title":"Medical Sciences Monitor"},{"key":"S0967199425000103_ref18","doi-asserted-by":"publisher","DOI":"10.1071\/RD18218"},{"key":"S0967199425000103_ref20","doi-asserted-by":"publisher","DOI":"10.1530\/REP-19-0501"},{"key":"S0967199425000103_ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2023.03.016"},{"key":"S0967199425000103_ref33","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11040745"},{"key":"S0967199425000103_ref38","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2023.108490"},{"key":"S0967199425000103_ref41","doi-asserted-by":"publisher","DOI":"10.1016\/j.domaniend.2023.106824"},{"key":"S0967199425000103_ref8","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13565"},{"key":"S0967199425000103_ref3","doi-asserted-by":"publisher","DOI":"10.3390\/cimb45030155"},{"key":"S0967199425000103_ref4","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12080"},{"key":"S0967199425000103_ref39","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2009.03.002"},{"key":"S0967199425000103_ref44","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E19-12-0709"},{"key":"S0967199425000103_ref51","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2018.05.033"},{"key":"S0967199425000103_ref52","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2015.04.008"},{"key":"S0967199425000103_ref53","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-019-0509-4"},{"key":"S0967199425000103_ref40","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2022.107186"},{"key":"S0967199425000103_ref30","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-023-03794-2"},{"key":"S0967199425000103_ref34","doi-asserted-by":"publisher","DOI":"10.32604\/biocell.2021.011827"},{"key":"S0967199425000103_ref6","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-37086-0"},{"key":"S0967199425000103_ref17","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dead008"},{"key":"S0967199425000103_ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2022.102848"},{"key":"S0967199425000103_ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2015.11.015"},{"key":"S0967199425000103_ref2","doi-asserted-by":"publisher","DOI":"10.1002\/iub.1646"},{"key":"S0967199425000103_ref31","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.5309"},{"key":"S0967199425000103_ref35","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12256"},{"key":"S0967199425000103_ref42","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2023.05.027"},{"key":"S0967199425000103_ref46","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2013.03.043"},{"key":"S0967199425000103_ref36","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7113-8_26"},{"key":"S0967199425000103_ref10","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199421000824"},{"key":"S0967199425000103_ref13","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioz215"},{"key":"S0967199425000103_ref14","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9090867"},{"key":"S0967199425000103_ref16","doi-asserted-by":"publisher","DOI":"10.1530\/REP-13-0251"},{"key":"S0967199425000103_ref21","doi-asserted-by":"publisher","DOI":"10.3390\/ani12151957"},{"key":"S0967199425000103_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2020.11.003"},{"key":"S0967199425000103_ref28","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2023.11860"},{"key":"S0967199425000103_ref29","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"S0967199425000103_ref32","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24031839"},{"key":"S0967199425000103_ref1","doi-asserted-by":"publisher","DOI":"10.1007\/s10561-020-09844-1"},{"key":"S0967199425000103_ref37","doi-asserted-by":"publisher","DOI":"10.21451\/1984-3143-AR2018-0140"},{"key":"S0967199425000103_ref47","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/den070"},{"key":"S0967199425000103_ref26","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2021.06.050"},{"key":"S0967199425000103_ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2021.07.005"},{"key":"S0967199425000103_ref48","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2015.11.001"},{"key":"S0967199425000103_ref24","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-5198(19)30606-7"},{"key":"S0967199425000103_ref22","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0073875"}],"container-title":["Zygote"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0967199425000103","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T08:14:49Z","timestamp":1762244089000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0967199425000103\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":52,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["S0967199425000103"],"URL":"https:\/\/doi.org\/10.1017\/s0967199425000103","relation":{},"ISSN":["0967-1994","1469-8730"],"issn-type":[{"type":"print","value":"0967-1994"},{"type":"electronic","value":"1469-8730"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"\u00a9 The Author(s), 2025. Published by Cambridge University Press","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}