{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:29:04Z","timestamp":1785421744888,"version":"3.56.0"},"reference-count":46,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T00:00:00Z","timestamp":1764892800000},"content-version":"unspecified","delay-in-days":65,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Centromeres are chromosomal loci essential for the correct segregation of genetic material during cell division. Defects in centromere function can lead to aneuploidy and cancer. During early embryonic development in mammals, prior to the first cell division, male and female genomes are separated in pronuclei located at the centre of the zygote. Parental chromatin clusters at the interface between the two pronuclei and this clustering step is critical to avoid aneuploidy in human and bovine zygotes. Yet, despite their essential function in chromosome segregation, the position and spatial organization of centromeres during the first cell cycle in mammals is mostly unknown. Previous studies conducted in bovine embryos derived from\n                    <jats:italic>in vitro<\/jats:italic>\n                    fertilization (IVF) showed that cell cycle progression impacts on the success rate of blastocyst formation. Specifically, embryos that entered earliest into S-phase or the earliest cleaving embryos were more likely to develop into blastocysts. To determine the precise timing of these events we performed a detailed characterization of key phases of the first cell cycle in bovine zygotes derived from IVF. In parallel we examined the spatial positioning of centromeres. We identify 20 h post insemination (hpi) as the timepoint when male and female pronuclei are juxtaposed and are completing S-phase. At this timepoint, we show that centromeres are positioned distal to the pronuclear interface and use super resolution microscopy to demonstrate extensive centromere clustering into chromocentres. Our results identify distinct nuclear features observed at 20 hpi, which may serve as cell cycle markers in determining successful bovine IVF.\n                  <\/jats:p>","DOI":"10.1017\/s0967199425100221","type":"journal-article","created":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T07:21:35Z","timestamp":1764919295000},"page":"257-264","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":1,"title":["Cell cycle timing and centromere positioning in\n                    <i>Bos taurus<\/i>\n                    zygotes derived from\n                    <i>in vitro<\/i>\n                    fertilization"],"prefix":"10.1017","volume":"33","author":[{"given":"Federica","family":"Modafferi","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03bea9k73","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Galway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Thompson","sequence":"additional","affiliation":[{"name":"University College Dublin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caitr\u00edona M.","family":"Collins","sequence":"additional","affiliation":[{"name":"Technological University of the Shannon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"O\u2019Callaghan","sequence":"additional","affiliation":[{"name":"University College Dublin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Lonergan","sequence":"additional","affiliation":[{"name":"University College Dublin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6885-0421","authenticated-orcid":false,"given":"Elaine M.","family":"Dunleavy","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03bea9k73","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Galway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2025,12,5]]},"reference":[{"key":"S0967199425100221_ref14","doi-asserted-by":"crossref","first-page":"2210","DOI":"10.1038\/s41467-018-04678-8","article-title":"A novel atypical sperm centriole is functional during human fertilization","volume":"9","author":"Fishman","year":"2018","journal-title":"Nature Communications"},{"key":"S0967199425100221_ref39","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1038\/s41576-018-0087-x","article-title":"Mechanisms regulating zygotic genome activation","volume":"20","author":"Schulz","year":"2019","journal-title":"Nature Reviews Genetics"},{"key":"S0967199425100221_ref35","doi-asserted-by":"crossref","first-page":"12","DOI":"10.3390\/biology6010012","article-title":"The consequences of chromosome segregation errors in mitosis and meiosis","volume":"6","author":"Potapova","year":"2017","journal-title":"Biology"},{"key":"S0967199425100221_ref45","doi-asserted-by":"crossref","unstructured":"Wiltbank, M.C. , Baez, G.M. , Garcia-Guerra, A. , Toledo, M.Z. , Monteiro, P.L.J. , Melo, L.F. , Ochoa, J.C. , Santos, J.E.P. and Sartori, R. , (2016) Pivotal periods for pregnancy loss during the first trimester of gestation in lactating dairy cows. Theriogenology, Proceedings of the 18th ICAR 86, 239\u2013253. https:\/\/doi.org\/10.1016\/j.theriogenology.2016.04.037","DOI":"10.1016\/j.theriogenology.2016.04.037"},{"key":"S0967199425100221_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.04.013"},{"key":"S0967199425100221_ref27","first-page":"113","volume-title":"Current Topics in Developmental Biology, Gastrulation: From Embryonic Pattern to Form","author":"Mol\u00e8","year":"2020"},{"key":"S0967199425100221_ref28","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/j.tig.2019.05.003","article-title":"The impact of centromeres on spatial genome architecture","volume":"35","author":"Muller","year":"2019","journal-title":"Trends in Genetics"},{"key":"S0967199425100221_ref10","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evz061"},{"key":"S0967199425100221_ref3","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-022-00517-3"},{"key":"S0967199425100221_ref29","doi-asserted-by":"crossref","first-page":"5","DOI":"10.3390\/biology6010005","article-title":"A molecular view of kinetochore assembly and function","volume":"6","author":"Musacchio","year":"2017","journal-title":"Biology"},{"key":"S0967199425100221_ref34","doi-asserted-by":"publisher","DOI":"10.1515\/znc-1985-3-418"},{"key":"S0967199425100221_ref4","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod62.6.1677"},{"key":"S0967199425100221_ref9","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod51.6.1232"},{"key":"S0967199425100221_ref15","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1530\/jrf.0.0860637","article-title":"Qualitative and quantitative changes in protein synthesis occur at the 8\u201316-cell stage of embryogenesis in the cow","volume":"86","author":"Frei","year":"1989","journal-title":"Reproduction"},{"key":"S0967199425100221_ref26","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1038\/nrm.2015.5","article-title":"The molecular basis for centromere identity and function","volume":"17","author":"McKinley","year":"2016","journal-title":"Nature Reviews Molecular Cell Biology"},{"key":"S0967199425100221_ref32","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.06.028"},{"key":"S0967199425100221_ref40","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1017\/S1751731114000615","article-title":"Effect of fertility on the economics of pasture-based dairy systems","volume":"8","author":"Shalloo","year":"2014","journal-title":"Animal"},{"key":"S0967199425100221_ref42","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cancerbio-062722-021823"},{"key":"S0967199425100221_ref24","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1530\/jrf.0.1170159","article-title":"Effect of time interval from insemination to first cleavage on the developmental characteristics, sex ratio and pregnancy rate after transfer of bovine embryos","volume":"117","author":"Lonergan","year":"1999","journal-title":"Reproduction"},{"key":"S0967199425100221_ref44","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1060"},{"key":"S0967199425100221_ref12","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1080\/09513590.2017.1388365","article-title":"Pronuclear pattern does not predict morphokinetics behavior in human embryos","volume":"34","author":"Faramarzi","year":"2018","journal-title":"Gynecological Endocrinology"},{"key":"S0967199425100221_ref19","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/BF00305013","article-title":"Ultrastructural abnormalities of in vitro fertilization of in vitro matured bovine oocytes","volume":"178","author":"Hyttel","year":"1988","journal-title":"Anatomy and Embryology(Berl.)"},{"key":"S0967199425100221_ref22","doi-asserted-by":"crossref","first-page":"1707","DOI":"10.1095\/biolreprod.103.017178","article-title":"Cell cycle duration at the time of maternal zygotic transition for in vitro produced bovine embryos: effect of oxygen tension and transcription inhibition1","volume":"69","author":"Lequarre","year":"2003","journal-title":"Biology of Reproduction"},{"key":"S0967199425100221_ref33","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/0022-2836(82)90434-X","article-title":"Nucleotide sequence of bovine 1.715 satellite DNA and its relation to other bovine satellite sequences","volume":"158","author":"P\u0142lucienniczak","year":"1982","journal-title":"Journal of Molecular Biology"},{"key":"S0967199425100221_ref17","doi-asserted-by":"publisher","DOI":"10.1007\/BF00319585"},{"key":"S0967199425100221_ref36","unstructured":"Rasband, W.S. , (1997) ImageJ, U.S. National Institutes of Health. Httprsbinfonihgovij 1997-2007."},{"key":"S0967199425100221_ref38","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nature Methods"},{"key":"S0967199425100221_ref41","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1093\/humrep\/12.7.1531","article-title":"Early cleavage of in-vitro fertilized human embryos to the 2-cell stage: a novel indicator of embryo quality and viability","volume":"12","author":"Shoukir","year":"1997","journal-title":"Human Reproduction (Oxford, England)"},{"key":"S0967199425100221_ref43","doi-asserted-by":"crossref","first-page":"RD24139","DOI":"10.1071\/RD24139","article-title":"Morphokinetic prediction of embryo viability in cattle","volume":"37","author":"Sugimura","year":"2024","journal-title":"Reproduction, Fertility and Development"},{"key":"S0967199425100221_ref46","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1038\/nbt.1686","article-title":"Non-invasive imaging of human embryos before embryonic genome activation predicts development to the blastocyst stage","volume":"28","author":"Wong","year":"2010","journal-title":"Nature Biotechnology"},{"key":"S0967199425100221_ref25","doi-asserted-by":"crossref","unstructured":"Manske, G. , Jorgensen, K. , Ma, B. , Aboelenain, M. , Tower, C. , Chakraborty, S. , Ranjan, R. , Das, A. , Lampson, M.A. , Black, B.E. , Schindler, K. , Chen, X. and Hammoud, S.S. , (2022) Equalizing epigenetically imprinted centromeres in early mammalian embryos. https:\/\/doi.org\/10.1101\/2022.10.27.514094","DOI":"10.1101\/2022.10.27.514094"},{"key":"S0967199425100221_ref11","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1080\/24750263.2018.1558294","article-title":"Bovine satellite DNAs \u2013 a history of the evolution of complexity and its impact in the Bovidae family","volume":"86","author":"Escudeiro","year":"2019","journal-title":"The European Zoological Journal"},{"key":"S0967199425100221_ref1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abl4178"},{"key":"S0967199425100221_ref23","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-animal-022114-110822"},{"key":"S0967199425100221_ref5","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2024-25139"},{"key":"S0967199425100221_ref16","doi-asserted-by":"crossref","first-page":"4654","DOI":"10.1038\/s41467-020-18508-3","article-title":"Chromatin remodeling in bovine embryos indicates species-specific regulation of genome activation","volume":"11","author":"Halstead","year":"2020","journal-title":"Nature Communications"},{"key":"S0967199425100221_ref21","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S1642-431X(12)60002-3","article-title":"Timing of the first zygotic cleavage as a marker of developmental potential of mammalian embryos","volume":"8","author":"Lechniak","year":"2008","journal-title":"Reproductive Biology"},{"key":"S0967199425100221_ref30","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1006\/dbio.1994.1064","article-title":"Microtubule organization in the cow during fertilization, polyspermy, parthenogenesis and nuclear transfer: the role of the sperm aster","volume":"162","author":"Navara","year":"1994","journal-title":"Developmental Biology"},{"key":"S0967199425100221_ref37","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gaw058"},{"key":"S0967199425100221_ref31","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1083\/jcb.116.5.1095","article-title":"Dynamic organization of DNA replication in mammalian cell nuclei: spatially and temporally defined replication of chromosome-specific alpha-satellite DNA sequences","volume":"116","author":"O\u2019Keefe","year":"1992","journal-title":"The Journal of Cell Biology"},{"key":"S0967199425100221_ref6","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-022-00897-w"},{"key":"S0967199425100221_ref8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2008.01171.x"},{"key":"S0967199425100221_ref7","doi-asserted-by":"publisher","DOI":"10.1101\/gr.200527.115"},{"key":"S0967199425100221_ref20","doi-asserted-by":"crossref","first-page":"200051","DOI":"10.1098\/rsob.200051","article-title":"The centromere comes into focus: from CENP-A nucleosomes to kinetochore connections with the spindle","volume":"10","author":"Kixmoeller","year":"2020","journal-title":"Open Biology"},{"key":"S0967199425100221_ref18","first-page":"35","article-title":"Ultrastructural aspects of oocyte maturation and fertilization in cattle","volume":"38","author":"Hyttel","year":"1989","journal-title":"Journal of Reproduction and Fertility. Supplement"},{"key":"S0967199425100221_ref13","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1017\/S1751731119002775","article-title":"Review: recent advances in bovine in vitro embryo production: reproductive biotechnology history and methods","volume":"14","author":"Ferr\u00e9","year":"2020","journal-title":"Animal"}],"container-title":["Zygote"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0967199425100221","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T05:15:42Z","timestamp":1768194942000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0967199425100221\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":46,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["S0967199425100221"],"URL":"https:\/\/doi.org\/10.1017\/s0967199425100221","relation":{},"ISSN":["0967-1994","1469-8730"],"issn-type":[{"value":"0967-1994","type":"print"},{"value":"1469-8730","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"\u00a9 The Author(s), 2025. Published by Cambridge University Press","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (https:\/\/creativecommons.org\/licenses\/by\/4.0\/), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}