{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:58:24Z","timestamp":1781107104076,"version":"3.54.1"},"reference-count":28,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:00:00Z","timestamp":1778544000000},"content-version":"unspecified","delay-in-days":41,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Long-term trophectoderm (TE) cell culture provides a powerful model to investigate placenta-specific factors to better understand mechanisms relevant to pregnancy establishment and placental development. However, current TE culture systems rely on costly commercial media and extracellular matrix (ECM) components, which limit their scalability and accessibility. This study evaluated cost-effective alternatives to established conditions by testing modified DMEM\/F-12 and a biphasic TE culture system as substitutes for commercial Advanced DMEM\/F-12 and for continuous TE culture, and by assessing 0.1% gelatine as an ECM alternative to collagen IV. Trophectoderm outgrowths cultured on collagen IV or gelatine did not differ in attachment timing (\n                    <jats:italic>P = 0.78<\/jats:italic>\n                    ) or in expression of placental and differentiation markers\n                    <jats:italic>PLAC8<\/jats:italic>\n                    (\n                    <jats:italic>P = 0.78<\/jats:italic>\n                    ),\n                    <jats:italic>GATA2<\/jats:italic>\n                    (\n                    <jats:italic>P = 0.18<\/jats:italic>\n                    ), and\n                    <jats:italic>PAG10<\/jats:italic>\n                    (\n                    <jats:italic>P = 0.39<\/jats:italic>\n                    ). Similarly, blastocysts cultured in commercial Advanced DMEM\/F-12 or base DMEM\/F-12 exhibited no differences in attachment day (\n                    <jats:italic>P = 0.98<\/jats:italic>\n                    ), TE growth area from days 10\u201320 (\n                    <jats:italic>P<\/jats:italic>\n                    &gt; 0.05), or expression of\n                    <jats:italic>PLAC8<\/jats:italic>\n                    (\n                    <jats:italic>P<\/jats:italic>\n                    = 0.35) and\n                    <jats:italic>PAG10<\/jats:italic>\n                    (\n                    <jats:italic>P<\/jats:italic>\n                    = 0.08), although\n                    <jats:italic>PAG7<\/jats:italic>\n                    differed between treatments (\n                    <jats:italic>P &lt; 0.05<\/jats:italic>\n                    ). Embryos cultured in continuous TE attached later (\n                    <jats:italic>P = 0.01<\/jats:italic>\n                    ) than those cultured in TE media, but no differences were observed in TE growth area or expression of\n                    <jats:italic>PLAC8<\/jats:italic>\n                    ,\n                    <jats:italic>PAG7<\/jats:italic>\n                    , or\n                    <jats:italic>PAG10<\/jats:italic>\n                    (\n                    <jats:italic>P &gt; 0.05<\/jats:italic>\n                    ). Collectively, these results indicate that affordable media formulations and gelatine-coated cultureware support TE attachment, proliferation, and differentiation. This cost-effective culture framework enables broader application of TE models and supports extended studies of trophoblast function, placental signalling, and early conceptus development.\n                  <\/jats:p>","DOI":"10.1017\/s096719942610046x","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T04:01:25Z","timestamp":1778558485000},"page":"98-104","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":0,"title":["Cost-effective strategies for long-term bovine trophectoderm cell culture"],"prefix":"10.1017","volume":"34","author":[{"given":"Ethel Sofia","family":"Moreno","sequence":"first","affiliation":[{"name":"University of Wisconsin-Madison"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Briza","family":"Castro","sequence":"additional","affiliation":[{"name":"University of Wisconsin-Madison"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4027-7314","authenticated-orcid":false,"given":"M. Sofia","family":"Ortega","sequence":"additional","affiliation":[{"name":"University of Wisconsin-Madison"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2026,5,12]]},"reference":[{"key":"S096719942610046X_ref24","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.905438"},{"key":"S096719942610046X_ref7","first-page":"179","volume-title":"Placenta and Trophoblast: Methods and Protocols","volume":"1","author":"Hashizume","year":"2006"},{"key":"S096719942610046X_ref20","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(84)81636-7"},{"key":"S096719942610046X_ref19","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod62.2.235"},{"key":"S096719942610046X_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.07.011"},{"key":"S096719942610046X_ref11","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178041"},{"key":"S096719942610046X_ref18","volume-title":"Hydroxyapatite Coatings for Biomedical Applications","author":"Qiang","year":"2013"},{"key":"S096719942610046X_ref23","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3516"},{"key":"S096719942610046X_ref8","first-page":"135","article-title":"Review: Gelatin, source, extraction and industrial applications","volume":"12","author":"Mariod","year":"2013","journal-title":"Acta Scientiarum Polonorum Technologia Alimentaria"},{"key":"S096719942610046X_ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.08.012"},{"key":"S096719942610046X_ref13","doi-asserted-by":"publisher","DOI":"10.3389\/fanim.2022.898808"},{"key":"S096719942610046X_ref27","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.02.005"},{"key":"S096719942610046X_ref1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.10-186K"},{"key":"S096719942610046X_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.05.079"},{"key":"S096719942610046X_ref4","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1915389116"},{"key":"S096719942610046X_ref17","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-15856-3_13"},{"key":"S096719942610046X_ref5","doi-asserted-by":"publisher","DOI":"10.1039\/C4BM00246F"},{"key":"S096719942610046X_ref10","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.23304"},{"key":"S096719942610046X_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2023.04.003"},{"key":"S096719942610046X_ref12","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioy141"},{"key":"S096719942610046X_ref21","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-9566-0_8"},{"key":"S096719942610046X_ref26","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.111.093203"},{"key":"S096719942610046X_ref3","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2012-5845"},{"key":"S096719942610046X_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2011.12.008"},{"key":"S096719942610046X_ref14","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/iox004"},{"key":"S096719942610046X_ref22","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-9566-0_8"},{"key":"S096719942610046X_ref16","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2013-6348"},{"key":"S096719942610046X_ref28","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0243727"}],"container-title":["Zygote"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S096719942610046X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:08:46Z","timestamp":1781104126000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S096719942610046X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":28,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["S096719942610046X"],"URL":"https:\/\/doi.org\/10.1017\/s096719942610046x","relation":{},"ISSN":["0967-1994","1469-8730"],"issn-type":[{"value":"0967-1994","type":"print"},{"value":"1469-8730","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"\u00a9 The Author(s), 2026. Published by Cambridge University Press","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (https:\/\/creativecommons.org\/licenses\/by\/4.0\/), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}