{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T10:07:12Z","timestamp":1789726032012,"version":"4.0.1"},"reference-count":17,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Dynamic systems for\n                    <jats:italic>in vitro<\/jats:italic>\n                    embryo production (IVEP) have long been sought to allow a higher resemblance to the physiological conditions of the oviduct and uterus. Although significant progress has been demonstrated in the microfluidic field, some challenges are still present regarding the microfluidic devices\u2019 sterilization protocol. This complication is related to the leached components from the poly(dimethylsiloxane) (PDMS). The PDMS is one of the safest materials used in soft lithography for embryo production. However, the use of moulds required for soft lithography can compromise embryo culture. This limitation arises from the restricted range of biocompatible resins available for 3D-printing of moulds. While the replacement of the biomaterial for mould production is not a straightforward option, we hypothesized that PDMS devices fabricated from such moulds could be rendered biocompatible through an optimized cleaning protocol. In this current study, we evaluated the effectiveness of sequential washes, sonication, and extended incubation in washing solutions. Our results demonstrate that a prior incubation of the PDMS devices in PBS supplemented with antibiotics and antifungal (at 4\u00b0C for 48 hours), in addition to the incubation in IVC medium (at 38.5\u00b0C for 48 hours), enables bovine embryo development within PDMS devices fabricated with moulds from a 3D printer. This optimized protocol supported IVEP at rates equivalent to standard conditions, with no increase in apoptosis or oxidative stress, validating its suitability for artificial reproductive techniques applications.\n                  <\/jats:p>","DOI":"10.1017\/s0967199426100550","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T05:42:07Z","timestamp":1784612527000},"page":"167-172","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":0,"title":["Establishing a safe and effective cleaning method for PDMS devices derived from 3D-printed resin moulds in embryo production"],"prefix":"10.1017","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4280-9575","authenticated-orcid":false,"given":"Patricia","family":"Kubo Fontes","sequence":"first","affiliation":[{"name":"Federal University of the ABC Centre for Natural Sciences and Humanities"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roksan","family":"Franko","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05591te55","id-type":"ROR","asserted-by":"publisher"}],"name":"Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuliana","family":"de Avila Ferronato","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05591te55","id-type":"ROR","asserted-by":"publisher"}],"name":"Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcella","family":"Milazzotto","sequence":"additional","affiliation":[{"name":"Federal University of the ABC Centre for Natural Sciences and Humanities"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcia","family":"de Almeida Monteiro Melo Ferraz","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05591te55","id-type":"ROR","asserted-by":"publisher"}],"name":"Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen"},{"id":[{"id":"https:\/\/ror.org\/04et42c10","id-type":"ROR","asserted-by":"publisher"}],"name":"Toronto Zoo"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2026,7,21]]},"reference":[{"key":"S0967199426100550_ref8","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07119-8"},{"key":"S0967199426100550_ref13","doi-asserted-by":"publisher","DOI":"10.1021\/ac061990v"},{"key":"S0967199426100550_ref12","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dep449"},{"key":"S0967199426100550_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2021.130820"},{"key":"S0967199426100550_ref2","doi-asserted-by":"publisher","DOI":"10.1039\/c2lc20982a"},{"key":"S0967199426100550_ref3","doi-asserted-by":"publisher","DOI":"10.1039\/C6LC00163G"},{"key":"S0967199426100550_ref15","unstructured":"McKenna, C. (2016). Efficient cleaning of a microfluidic chip. Available at https:\/\/blogs.rsc.org\/chipsandtips\/2016\/02\/08\/efficient-cleaning-of-a-microfluidic-chip\/?doing_wp_cron=1710781933.4187800884246826171875 (accessed 18 March 2024)."},{"key":"S0967199426100550_ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2021.07.010"},{"key":"S0967199426100550_ref10","doi-asserted-by":"publisher","DOI":"10.1039\/D4LC00660G"},{"key":"S0967199426100550_ref1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2023.1260886"},{"key":"S0967199426100550_ref11","doi-asserted-by":"publisher","DOI":"10.1098\/rsif.2021.0929"},{"key":"S0967199426100550_ref17","doi-asserted-by":"publisher","DOI":"10.3390\/s21051754"},{"key":"S0967199426100550_ref7","doi-asserted-by":"publisher","DOI":"10.1039\/C6LC01566B"},{"key":"S0967199426100550_ref14","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200900157"},{"key":"S0967199426100550_ref5","doi-asserted-by":"publisher","DOI":"10.1021\/acs.estlett.7b00495"},{"key":"S0967199426100550_ref9","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioaf202"},{"key":"S0967199426100550_ref6","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-57816-y"}],"container-title":["Zygote"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0967199426100550","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T09:31:49Z","timestamp":1789723909000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0967199426100550\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,21]]},"references-count":17,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["S0967199426100550"],"URL":"https:\/\/doi.org\/10.1017\/s0967199426100550","relation":{},"ISSN":["0967-1994","1469-8730"],"issn-type":[{"value":"0967-1994","type":"print"},{"value":"1469-8730","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,21]]},"assertion":[{"value":"\u00a9 The Author(s), 2026. Published by Cambridge University Press","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (https:\/\/creativecommons.org\/licenses\/by\/4.0\/), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}