{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T09:51:02Z","timestamp":1791798662169,"version":"4.3.4"},"reference-count":12,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Trafficking Protein Particle Complex Subunit 4 (TRAPPC4) is essential for protein transport from the endoplasmic reticulum to the Golgi apparatus. It plays a crucial role in tethering COPII vesicles and assembling SNARE complexes, ensuring precise protein delivery, which is vital for cellular communication and growth. Mutations in TRAPPC4 and other TRAPP subunits are associated with a group of human diseases collectively known as TRAPPopathies, often manifesting as neurological disorders. This study investigates the role of TRAPPC4 during early embryonic development by analysing the phenotypic consequences when the gene is knocked out in the mouse. Our results show that TRAPPC4 knockout leads to early embryonic lethality, where TRAPPC4 homozygous mutant embryos form morphologically normal blastocysts but fail to implant in the uterus and are not present at embryonic day 7.5. We show that mutant embryos can hatch from the zona pellucida, but do not form proper inner cell mass (ICM) outgrowths. Unlike the tightly compressed ICM colonies in control littermates, the mutant ICM appears necrotic with cells that are not adherent. This dispersion suggests that ICM cells may begin deteriorating at peri-implantation stages, underscoring TRAPPC4\u2019s pivotal role in maintaining cellular viability during early embryonic development.<\/jats:p>","DOI":"10.1017\/s0967199426100598","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T06:21:27Z","timestamp":1789107687000},"page":"234-239","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":0,"title":["Trappc4 is required for early mammalian development"],"prefix":"10.1017","volume":"34","author":[{"given":"Sarah S.","family":"Mirza","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0072zz521","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Massachusetts Amherst"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise","family":"Bassom","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0072zz521","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Massachusetts Amherst"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minjun","family":"Ahn","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0072zz521","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Massachusetts Amherst"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4123-0633","authenticated-orcid":false,"given":"Jesse","family":"Mager","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0072zz521","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Massachusetts Amherst"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"key":"S0967199426100598_ref8","doi-asserted-by":"publisher","DOI":"10.1101\/gr.978703"},{"key":"S0967199426100598_ref2","first-page":"e109784","article-title":"Impact of euploid embryo transfer and maternal age on implantation outcomes in in vitro fertilization-embryo transfer (IVF-ET): A systematic review","volume":"8","author":"Belal","year":"2026","journal-title":"Cureus"},{"key":"S0967199426100598_ref12","doi-asserted-by":"publisher","DOI":"10.1038\/srep43207"},{"key":"S0967199426100598_ref10","unstructured":"Online Mendelian Inheritance in Man, OMIM\u00ae . (2023) Johns Hopkins University, Baltimore, MD. MIM Number: 618741. World Wide Web. Available at https:\/\/www.omim.org\/entry\/610971?search=trappc4&highlight=trappc4 (accessed 09 August 2026)."},{"key":"S0967199426100598_ref4","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00003.2012"},{"key":"S0967199426100598_ref5","doi-asserted-by":"publisher","DOI":"10.1038\/nature19356"},{"key":"S0967199426100598_ref7","doi-asserted-by":"publisher","DOI":"10.3390\/ijms252413329"},{"key":"S0967199426100598_ref1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.112.142810"},{"key":"S0967199426100598_ref6","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.14553"},{"key":"S0967199426100598_ref11","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201705068"},{"key":"S0967199426100598_ref9","unstructured":"Mager Lab (2025) Early gene expression. Available at https:\/\/websites.umass.edu\/jmager\/early-gene-expression\/ (accessed 01 March 2026)."},{"key":"S0967199426100598_ref3","volume-title":"Anembryonic Pregnancy","author":"Carlson","year":"2026"}],"container-title":["Zygote"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0967199426100598","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T09:41:03Z","timestamp":1791798063000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0967199426100598\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,11]]},"references-count":12,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["S0967199426100598"],"URL":"https:\/\/doi.org\/10.1017\/s0967199426100598","relation":{},"ISSN":["0967-1994","1469-8730"],"issn-type":[{"value":"0967-1994","type":"print"},{"value":"1469-8730","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,11]]},"assertion":[{"value":"\u00a9 The Author(s), 2026. Published by Cambridge University Press","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (https:\/\/creativecommons.org\/licenses\/by\/4.0\/), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}