{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T09:51:01Z","timestamp":1791798661842,"version":"4.3.4"},"reference-count":58,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The aim of this study was to investigate the chemical composition of\n                    <jats:italic>Lippia sidoides<\/jats:italic>\n                    essential oil (LSEO) and the influence of different concentrations of LSEO on activation and survival of primordial follicles, ovarian stromal cell density, collagen area in the extracellular matrix (ECM), as well as on thiol levels, messenger RNA (mRNA) of CAT, SOD1, GPX1 and PRDX6 and activity of antioxidant enzymes CAT, SOD1 and GPX1. To this end, the chemical composition of the oil was analysed using the gas chromatography (GC) method. The bovine ovaries were collected from a local slaughterhouse. In the laboratory, fragments of the ovarian cortex were then cultured in control medium alone or in the presence of 400, 800, 1600 and 3200 \u00b5g\/mL LSEO. After six days of culture, the tissues were fixed and sent for histological analysis. Other fragments were used in molecular tests to determine the redox profile and to quantify mRNA levels. The results showed that a concentration of 400 \u03bcg\/mL LSEO promoted follicular activation and growth, as well as increased stromal cell density and the mRNA levels of SOD1, CAT, and GPX1. Additionally, the LSEO at all tested concentrations reduced CAT activity in cultured bovine ovarian tissues.\n                  <\/jats:p>","DOI":"10.1017\/s0967199426100616","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T08:42:07Z","timestamp":1790757727000},"page":"240-249","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":0,"title":["<i>Lippia sidoides<\/i>\n                    essential oil: chemical composition and antioxidant activity during\n                    <i>in vitro<\/i>\n                    culture of bovine ovarian tissue"],"prefix":"10.1017","volume":"34","author":[{"given":"Francisco Freire","family":"Caetano Filho","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03srtnf24","id-type":"ROR","asserted-by":"publisher"}],"name":"Federal University of Ceara"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vit\u00f3ria Santos","family":"Bezerra","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03srtnf24","id-type":"ROR","asserted-by":"publisher"}],"name":"Federal University of Ceara"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"La\u00eds R. 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Delta"},{"name":"State University of Cear\u00e1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Solano Dantas","family":"Martins","sequence":"additional","affiliation":[{"name":"State University of Ceara"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7045-1523","authenticated-orcid":false,"given":"Francisco das Chagas","family":"Costa","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03srtnf24","id-type":"ROR","asserted-by":"publisher"}],"name":"Federal University of Ceara"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5970-6177","authenticated-orcid":false,"given":"Jose Roberto V.","family":"Silva","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03srtnf24","id-type":"ROR","asserted-by":"publisher"}],"name":"Federal University of 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S\u00e3o Paulo State University (UNESP), School of Veterinary Medicine and Animal Science, Botucatu, Brazil, Available at https:\/\/acervodigital.unesp.br\/handle\/11449\/138464 (accessed 4 September 2024)."},{"key":"S0967199426100616_ref47","doi-asserted-by":"publisher","DOI":"10.1016\/S0308-8146(99)00247-2"},{"key":"S0967199426100616_ref61","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.30468"},{"key":"S0967199426100616_ref36","first-page":"668","article-title":"Justicia insularis improves the in vitro survival and development of ovine preantral follicles enclosed in ovarian tissue","volume":"5","author":"Mbemya","year":"2017","journal-title":"Journal of Pharmacy and Pharmacology"},{"key":"S0967199426100616_ref43","doi-asserted-by":"publisher","DOI":"10.1016\/j.domaniend.2015.09.002"},{"key":"S0967199426100616_ref41","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/9613090"},{"key":"S0967199426100616_ref37","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gay002"},{"key":"S0967199426100616_ref45","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-020-02450-z"},{"key":"S0967199426100616_ref22","doi-asserted-by":"publisher","DOI":"10.3390\/ani13010018"},{"key":"S0967199426100616_ref51","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2022.822367"},{"key":"S0967199426100616_ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2025.01.020"},{"key":"S0967199426100616_ref17","first-page":"365","article-title":"Aloe vera increases collagen fibres in extracellular matrix and mRNA expression of peroxiredoxin-6 in bovine ovarian cortical tissues cultured in vitro","volume":"3","author":"Costa","year":"2021","journal-title":"Zygote"},{"key":"S0967199426100616_ref26","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(84)05015-1"},{"key":"S0967199426100616_ref25","first-page":"248","article-title":"Artificial ovary technology: Applications, state of art, limitations and prospects","volume":"41","author":"Figueiredo","year":"2017","journal-title":"Animal Reproduction"},{"key":"S0967199426100616_ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2018.04.009"},{"key":"S0967199426100616_ref30","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26020307"},{"key":"S0967199426100616_ref38","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2006.11.036"},{"key":"S0967199426100616_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2024.107514"},{"key":"S0967199426100616_ref46","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7113-8_26"},{"key":"S0967199426100616_ref65","doi-asserted-by":"publisher","DOI":"10.3390\/ani14101443"},{"key":"S0967199426100616_ref8","first-page":"117","article-title":"Dynamic in vitro culture of bovine and human ovarian tissue enhances follicle progression and health","volume":"21","author":"Barbato","year":"2023","journal-title":"Scientific Reports"},{"key":"S0967199426100616_ref63","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.2c18734"},{"key":"S0967199426100616_ref64","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28052308"},{"key":"S0967199426100616_ref40","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26092621"}],"container-title":["Zygote"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0967199426100616","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T09:40:58Z","timestamp":1791798058000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0967199426100616\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,30]]},"references-count":58,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["S0967199426100616"],"URL":"https:\/\/doi.org\/10.1017\/s0967199426100616","relation":{},"ISSN":["0967-1994","1469-8730"],"issn-type":[{"value":"0967-1994","type":"print"},{"value":"1469-8730","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,30]]},"assertion":[{"value":"\u00a9 The Author(s), 2026. 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