{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T01:48:02Z","timestamp":1649209682771},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2001,4,5]],"date-time":"2001-04-05T00:00:00Z","timestamp":986428800000},"content-version":"unspecified","delay-in-days":1070,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zygote"],"published-print":{"date-parts":[[1998,5]]},"abstract":"<jats:p><jats:italic>In vitro<\/jats:italic> fertilisation (IVF) was used to assess the ability of solubilised sperm plasma membrane (PM) proteins to inhibit the interaction of intact gametes. This is a first step before evaluating the ability of individual isolated proteins to competitively inhibit sperm-oocyte interaction as part of the process of studying the molecular events of fertilisation. Porcine oocytes were aspirated from ovaries, matured for 48  h in Medium 199, and the zona pellucida (ZP) was removed by exposure to acid Tyrode's solution. ZP-free matured oocytes were exposed to 200\u2013800 \u03bcg\/ml sperm PM protein for 1 h prior to insemination and during gamete co-incubation. Twenty-four hours after insemination with 5 \u00d7 105 capacitated sperm\/ml, the oocytes were fixed, stained and examined. Sperm PM protein clearly inhibited IVF in a concentration-dependent manner (<jats:italic>r<\/jats:italic> = \u22120.87). The inhibition index (<jats:italic>I<\/jats:italic><jats:sub>50%<\/jats:sub>), representing the sperm PM protein concentration necessary to inhibit IVF to 50% of the control value, was 310 \u00b5g\/ml. These results demonstrate that solubilised sperm PM protein inhibits the interaction of intact gametes as one might expect for receptor-ligand interactions. Furthermore, the complement of sperm PM proteins appeared maximally effective at a calculated concentration of 690 \u00b5m\/ml, providing a foundation for further studies with individual proteins.<\/jats:p>","DOI":"10.1017\/s0967199498000082","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:39:08Z","timestamp":1027762748000},"page":"155-158","source":"Crossref","is-referenced-by-count":1,"title":["Sperm plasma membrane receptors for the porcine oocyte plasma membrane"],"prefix":"10.1017","volume":"6","author":[{"given":"Miguel","family":"Betancourt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvonne","family":"Ducolomb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irma","family":"Jim\u00e9nez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo","family":"Casas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edmundo","family":"Bonilla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trish","family":"Berger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2001,4,5]]},"container-title":["Zygote"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0967199498000082","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,9]],"date-time":"2019-04-09T16:31:46Z","timestamp":1554827506000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0967199498000082\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,5]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1998,5]]}},"alternative-id":["S0967199498000082"],"URL":"https:\/\/doi.org\/10.1017\/s0967199498000082","relation":{},"ISSN":["0967-1994","1469-8730"],"issn-type":[{"value":"0967-1994","type":"print"},{"value":"1469-8730","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,5]]}}}