{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T01:29:54Z","timestamp":1720574994757},"reference-count":20,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2017,12,4]],"date-time":"2017-12-04T00:00:00Z","timestamp":1512345600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Cardiol Young"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec id=\"S1047951117002311_abs1\" sec-type=\"general\"><jats:title>Background<\/jats:title><jats:p>Cyclooxygenase inhibitors are widely applied to facilitate ductal closure in preterm infants. The mechanisms that lead to patent ductus arteriosus closure are incompletely understood. Vascular endothelial growth factor plays pivotal roles during ductal closure and remodelling.<\/jats:p><\/jats:sec><jats:sec id=\"S1047951117002311_abs2\" sec-type=\"general\"><jats:title>Aim<\/jats:title><jats:p>The aim of this study was to investigate the effects of ibuprofen and indomethacin on the expression of vascular endothelial growth factor and its receptors in a primary rat ductus arteriosus endothelial cell culture.<\/jats:p><\/jats:sec><jats:sec id=\"S1047951117002311_abs3\" sec-type=\"methods\"><jats:title>Methods<\/jats:title><jats:p>Protein expression of vascular endothelial growth factor and vascular endothelial growth factor receptor 1 and 2 was confirmed in rat ductus arteriosus and aorta by immunofluorescence staining. Fetal rat endothelial cells were isolated from ductus arteriosus and aorta using immunomagnetic cell sorting and treated with ibuprofen or indomethacin. mRNA expression levels were assessed by quantitative polymerase chain reaction analysis.<\/jats:p><\/jats:sec><jats:sec id=\"S1047951117002311_abs4\" sec-type=\"results\"><jats:title>Results<\/jats:title><jats:p>In ductal endothelial cells, ibuprofen significantly induced vascular endothelial growth factor and its receptor 2, but not receptor 1, whereas indomethacin did not alter the expression levels of the vascular endothelial growth factor system. In contrast, ibuprofen significantly induced vascular endothelial growth factor and its receptors 1 and 2 in aortic endothelial cells, whereas indomethacin only induced vascular endothelial growth factor receptor 2.<\/jats:p><\/jats:sec><jats:sec id=\"S1047951117002311_abs5\" sec-type=\"conclusion\"><jats:title>Conclusion<\/jats:title><jats:p>Our results indicate differential effects of ibuprofen and indomethacin on the expression levels of the vascular endothelial growth factor system in ductus arteriosus endothelial cells. In addition, vessel-specific differences between ductal and aortic endothelial cells were found. Further in vivo studies are needed to elucidate the biological significance of these findings.<\/jats:p><\/jats:sec>","DOI":"10.1017\/s1047951117002311","type":"journal-article","created":{"date-parts":[[2017,12,3]],"date-time":"2017-12-03T23:57:00Z","timestamp":1512345420000},"page":"432-437","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":3,"title":["Ibuprofen and indomethacin differentially regulate vascular endothelial growth factor and its receptors in ductus arteriosus endothelial cells"],"prefix":"10.1017","volume":"28","author":[{"given":"Hannes","family":"Sallmon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Akanbi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sven C.","family":"Weber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Gratopp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cornelia","family":"Rheinl\u00e4nder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"Koehne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2017,12,4]]},"reference":[{"key":"S1047951117002311_ref11","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.06-0057"},{"key":"S1047951117002311_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clp.2015.11.008"},{"key":"S1047951117002311_ref18","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2005-2870"},{"key":"S1047951117002311_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2015.06.032"},{"key":"S1047951117002311_ref17","doi-asserted-by":"publisher","DOI":"10.1001\/jamapediatrics.2016.5143"},{"key":"S1047951117002311_ref8","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.77.3.638"},{"key":"S1047951117002311_ref7","doi-asserted-by":"publisher","DOI":"10.1203\/PDR.0b013e318225f748"},{"key":"S1047951117002311_ref9","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.18.6.2980"},{"key":"S1047951117002311_ref12","doi-asserted-by":"publisher","DOI":"10.1136\/archdischild-2014-306176"},{"key":"S1047951117002311_ref20","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00185.2005"},{"key":"S1047951117002311_ref1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2009-3506"},{"key":"S1047951117002311_ref5","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2060"},{"key":"S1047951117002311_ref3","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00298.2001"},{"key":"S1047951117002311_ref4","doi-asserted-by":"publisher","DOI":"10.1203\/PDR.0b013e318180a375"},{"key":"S1047951117002311_ref6","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2012-0499"},{"key":"S1047951117002311_ref10","doi-asserted-by":"publisher","DOI":"10.1203\/PDR.0b013e3182294471"},{"key":"S1047951117002311_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2015.08.023"},{"key":"S1047951117002311_ref15","first-page":"13","article-title":"Prophylactic indomethacin compared with delayed conservative management of the patent ductus arteriosus in extremely preterm infants: effects on neonatal outcomes","volume":"189","author":"Liebowitz","year":"2017","journal-title":"J Pediatr"},{"key":"S1047951117002311_ref16","doi-asserted-by":"publisher","DOI":"10.1186\/s12887-016-0762-z"},{"key":"S1047951117002311_ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2016.08.103"}],"container-title":["Cardiology in the Young"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S1047951117002311","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T15:55:08Z","timestamp":1555343708000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S1047951117002311\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,4]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,3]]}},"alternative-id":["S1047951117002311"],"URL":"https:\/\/doi.org\/10.1017\/s1047951117002311","relation":{},"ISSN":["1047-9511","1467-1107"],"issn-type":[{"value":"1047-9511","type":"print"},{"value":"1467-1107","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12,4]]},"assertion":[{"value":"\u00a9 Cambridge University Press 2017\u00a0","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}