{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T19:16:03Z","timestamp":1792696563581,"version":"4.5.0"},"reference-count":26,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T00:00:00Z","timestamp":1736121600000},"content-version":"unspecified","delay-in-days":5,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Cardiol Young"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec id=\"S104795112402688X_as1\"><jats:title>Purpose:<\/jats:title><jats:p>Studying the effect of interleukin-17 (IL-17) on the mechanism of CD4+ T-cell immune regulation and the Janus kinase\/signal transducer and activator of transcription (JAK\/STAT) pathway may offer new ideas and methods for the therapy of dilated cardiomyopathy.<\/jats:p><\/jats:sec><jats:sec id=\"S104795112402688X_as2\"><jats:title>Methods:<\/jats:title><jats:p>Naive CD4+ T cells were isolated from mice using a magnetic bead sorting reagent and manipulated by overexpression or knockdown of IL-17. Protein levels of Janus kinase 2 (JAK2), phosphorylated JAK2 (p-JAK2), signal transducer and activator of transcription 3 (STAT3), phosphorylated STAT3 (p-STAT3), matrix metalloproteinase-2 (MMP-2), and matrix metalloproteinase-9 (MMP-9) were determined by Western blotting. Quantitative polymerase chain reaction was used to assess the levels of JAK2, STAT3, MMP-2, and MMP-9. Expression of tumour necrosis factor-alpha (TNF-\u03b1), interleukin-1 beta (IL-1\u03b2), interleukin-4 (IL-4), and interferon-gamma (IFN\u03b3) was determined by enzyme-linked immunosorbent assay test kits. TNF-\u03b1, IL-1\u03b2, IL-4, and IFN\u03b3 secretion was measured by flow cytometry.<\/jats:p><\/jats:sec><jats:sec id=\"S104795112402688X_as3\"><jats:title>Results:<\/jats:title><jats:p>In CD4+ T cells, IL-17 overexpression increased TNF-\u03b1, IL-1\u03b2, IL-4, IFN\u03b3, p-JAK2, p-STAT3, MMP-2, MMP-9 levels, and apoptosis. Knockdown of IL-17 reduced the levels of TNF-\u03b1, IL-1\u03b2, IL-4, IFN\u03b3, p-JAK2, p-STAT3, MMP-2, and MMP-9, as well as the level of apoptosis.<\/jats:p><\/jats:sec><jats:sec id=\"S104795112402688X_as4\"><jats:title>Conclusion:<\/jats:title><jats:p>Through regulation of IL-17 expression in CD4+ T cells, this study reveals its crucial role in regulating the secretion of inflammatory factors, activation of the JAK\/STAT signaling pathway, expression of matrix metalloproteinases, and apoptosis of CD4+ T cells.<\/jats:p><\/jats:sec>","DOI":"10.1017\/s104795112402688x","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T06:29:39Z","timestamp":1736144979000},"page":"16-23","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":2,"title":["IL-17 affects the immune regulation of CD4+ T cells in dilated cardiomyopathy through JAK\/STAT pathway"],"prefix":"10.1017","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3601-9061","authenticated-orcid":false,"given":"Jie","family":"Hao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Jiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanan","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2025,1,6]]},"reference":[{"key":"S104795112402688X_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2019.08.002"},{"key":"S104795112402688X_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2013.10.021"},{"key":"S104795112402688X_ref3","doi-asserted-by":"publisher","DOI":"10.3390\/medicina59071246"},{"key":"S104795112402688X_ref11","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-8703(03)00445-9"},{"key":"S104795112402688X_ref9","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2018.00287"},{"key":"S104795112402688X_ref18","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.112.000434"},{"key":"S104795112402688X_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2017.02.079"},{"key":"S104795112402688X_ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbspin.2016.05.014"},{"key":"S104795112402688X_ref22","doi-asserted-by":"publisher","DOI":"10.1007\/s10517-018-4030-0"},{"key":"S104795112402688X_ref26","first-page":"125","article-title":"Matrix metalloproteinases in cardiovascular disease: mechanisms and therapeutic implications","volume":"75","author":"Wang","year":"2020","journal-title":"J Cardiovasc Pharmacol"},{"key":"S104795112402688X_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2015.09.011"},{"key":"S104795112402688X_ref10","first-page":"317","article-title":"Peripheral CD4+ T-cell differentiation regulated by networks of cytokines and transcription factors","volume":"240","author":"Zhu","year":"2011","journal-title":"Immunol Rev"},{"key":"S104795112402688X_ref13","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.309711"},{"key":"S104795112402688X_ref16","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201142209"},{"key":"S104795112402688X_ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.10.026"},{"key":"S104795112402688X_ref17","doi-asserted-by":"publisher","DOI":"10.2143\/AC.64.2.2036138"},{"key":"S104795112402688X_ref23","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.26.14376"},{"key":"S104795112402688X_ref24","first-page":"123","volume-title":"Interleukin-6 in Autoimmunity","author":"Kimura","year":"2017"},{"key":"S104795112402688X_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2016.03.590"},{"key":"S104795112402688X_ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejheart.2006.06.002"},{"key":"S104795112402688X_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2021.152153"},{"key":"S104795112402688X_ref15","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2005010117"},{"key":"S104795112402688X_ref21","doi-asserted-by":"publisher","DOI":"10.1097\/FJC.0000000000001007"},{"key":"S104795112402688X_ref25","first-page":"76","article-title":"The role of IL-17 and the Th17\/IL-17 axis in the pathogenesis of autoimmune diseases","volume":"21","author":"Martinez-Moreno","year":"2019","journal-title":"Curr Rheumatol Rep"},{"key":"S104795112402688X_ref2","doi-asserted-by":"publisher","DOI":"10.1111\/joim.12944"},{"key":"S104795112402688X_ref4","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.765107"}],"container-title":["Cardiology in the Young"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S104795112402688X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T05:10:42Z","timestamp":1738645842000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S104795112402688X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["S104795112402688X"],"URL":"https:\/\/doi.org\/10.1017\/s104795112402688x","relation":{},"ISSN":["1047-9511","1467-1107"],"issn-type":[{"value":"1047-9511","type":"print"},{"value":"1467-1107","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"\u00a9 The Author(s), 2025. Published by Cambridge University Press","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}