{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T10:47:20Z","timestamp":1792320440970,"version":"4.4.0"},"reference-count":13,"publisher":"Cambridge University Press (CUP)","issue":"8","license":[{"start":{"date-parts":[[2010,1,15]],"date-time":"2010-01-15T00:00:00Z","timestamp":1263513600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Public Health Nutr."],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec id=\"S1368980009992205_abs1\" sec-type=\"general\"><jats:title>Objective<\/jats:title><jats:p>There are few studies regarding vitamin B<jats:sub>12<\/jats:sub> deficiency in developing countries. In Brazil, a late diagnosis of vitamin B<jats:sub>12<\/jats:sub> deficiency progressing to severe neurological damage is common. Thus, the aim of the present study was to verify the frequency of vitamin B<jats:sub>12<\/jats:sub> deficiency in two Brazilian populations (elderly and adult participants) and to compare different methods of vitamin B<jats:sub>12<\/jats:sub> deficiency detection.<\/jats:p><\/jats:sec><jats:sec id=\"S1368980009992205_abs2\" sec-type=\"general\"><jats:title>Design<\/jats:title><jats:p>Five hundred participants were recruited from health centres from south-east Brazil and were separated into two groups: 60 years old or more and 30\u201359 years old. Vitamin B<jats:sub>12<\/jats:sub> and folate concentrations were measured using electrochemiluminescence immunoassay (ECI) and RIA. Methylmalonic acid (MMA) was measured by LC coupled to tandem MS. Full blood counts were acquired using standard methods.<\/jats:p><\/jats:sec><jats:sec id=\"S1368980009992205_abs3\" sec-type=\"results\"><jats:title>Results<\/jats:title><jats:p>All participants had normal blood count results and mean cell volume less than 99 fl; none of them presented folate deficiency according to the results, which were all greater than 3 ng\/ml. Cobalamin levels less than 200 pmol\/l were identified by one of the two or by both methods in 7\u00b72 % of the participants aged 60 years or more and 6\u00b74 % of the participants aged 30\u201359 years. MMA levels were higher in older subjects (<jats:italic>P<\/jats:italic> = 0\u00b7007) compared with younger subjects. A greater correlation of MMA <jats:italic>v<\/jats:italic>. RIA was observed than of MMA <jats:italic>v<\/jats:italic>. ECI (<jats:italic>P<\/jats:italic> = 0\u00b70017 <jats:italic>v<\/jats:italic>. <jats:italic>P<\/jats:italic> = 0\u00b7014). MMA quantification estimated that cobalamin deficiency was present in more than 11 % of the subjects for both studied groups.<\/jats:p><\/jats:sec><jats:sec id=\"S1368980009992205_abs4\" sec-type=\"conclusion\"><jats:title>Conclusions<\/jats:title><jats:p>The study shows that vitamin B<jats:sub>12<\/jats:sub> deficiency is frequent in Brazilian adults and suggests that RIA is more sensitive than ECl for measuring cobalamin levels.<\/jats:p><\/jats:sec>","DOI":"10.1017\/s1368980009992205","type":"journal-article","created":{"date-parts":[[2010,1,15]],"date-time":"2010-01-15T06:11:01Z","timestamp":1263535861000},"page":"1191-1197","source":"Crossref","is-referenced-by-count":13,"title":["High frequency of vitamin B<sub>12<\/sub> deficiency in a Brazilian population"],"prefix":"10.1017","volume":"13","author":[{"given":"JM","family":"Xavier","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FF","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JM","family":"Annichino-Bizzacchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"STO","family":"Saad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2010,1,15]]},"reference":[{"key":"S1368980009992205_ref5","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-04-1641"},{"key":"S1368980009992205_ref12","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-11-4293"},{"key":"S1368980009992205_ref1","doi-asserted-by":"publisher","DOI":"10.1093\/ageing\/afl033"},{"key":"S1368980009992205_ref9","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/137.7.1789"},{"key":"S1368980009992205_ref6","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/81.5.1155"},{"key":"S1368980009992205_ref2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/77.5.1241"},{"key":"S1368980009992205_ref3","doi-asserted-by":"publisher","DOI":"10.1017\/S0029665107005873"},{"key":"S1368980009992205_ref8","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2006.080382"},{"key":"S1368980009992205_ref10","first-page":"1591","article-title":"High frequency of suboptimal serum vitamin B12 level in adults in Jordan","volume":"26","author":"Fora","year":"2005","journal-title":"Saudi Med J"},{"key":"S1368980009992205_ref11","first-page":"334","article-title":"Folate and vitamin B12 status of adolescent girls in northern Nigeria","volume":"92","author":"VanderJagt","year":"2000","journal-title":"J Natl Med Assoc"},{"key":"S1368980009992205_ref13","first-page":"120","article-title":"Serum vitamin B12 levels in an Indian population: an evaluation of three assay methods","volume":"46","author":"Kumar","year":"1989","journal-title":"Med Lab Sci"},{"key":"S1368980009992205_ref4","doi-asserted-by":"publisher","DOI":"10.1157\/13112591"},{"key":"S1368980009992205_ref7","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.nutr.24.012003.132440"}],"container-title":["Public Health Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S1368980009992205","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,4]],"date-time":"2019-04-04T17:53:36Z","timestamp":1554400416000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S1368980009992205\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,1,15]]},"references-count":13,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2010,8]]}},"alternative-id":["S1368980009992205"],"URL":"https:\/\/doi.org\/10.1017\/s1368980009992205","relation":{},"ISSN":["1368-9800","1475-2727"],"issn-type":[{"value":"1368-9800","type":"print"},{"value":"1475-2727","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,1,15]]}}}