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A novel biological strategy of coupling intracellular biochemical reactions for controllable biosynthesis of CdSe quantum dots by living <jats:italic>B. subtilis<\/jats:italic> cells was demonstrated, through which highly luminant and photostable fluorescent <jats:italic>B. subtilis<\/jats:italic> cells were achieved with good uniformity. With the help of the obtained fluorescent <jats:italic>B. subtilis<\/jats:italic> cells probes, <jats:italic>S. aureus<\/jats:italic> cells responded to co-cultured <jats:italic>B. subtilis<\/jats:italic> and to aggregate. The degree of aggregation was calculated and nonlinearly fitted to a polynomial model. Systematic investigations of their interactions implied that <jats:italic>B. subtilis<\/jats:italic> cells inhibit the growth of neighboring <jats:italic>S. aureus<\/jats:italic> cells, and this inhibition was affected by both the growth stage and the amount of surrounding <jats:italic>B. subtilis<\/jats:italic> cells. Compared to traditional methods of studying bacterial interaction between two species, such as solid culture medium colony observation and imaging mass spectrometry detection, the procedures were more simple, vivid, and photostable due to the efficient fluorescence intralabeling with less influence on the cells\u2019 surface, which might provide a new paradigm for future visualization of microbial behavior.<\/jats:p>","DOI":"10.1017\/s1431927615015548","type":"journal-article","created":{"date-parts":[[2015,12,21]],"date-time":"2015-12-21T11:09:59Z","timestamp":1450696199000},"page":"13-21","source":"Crossref","is-referenced-by-count":12,"title":["Intracellular Biosynthesis of Fluorescent CdSe Quantum Dots in <i>Bacillus subtilis:<\/i> A Strategy to Construct Signaling Bacterial Probes for Visually Detecting Interaction Between <i>Bacillus subtilis<\/i> and <i>Staphylococcus aureus<\/i>"],"prefix":"10.1093","volume":"22","author":[{"given":"Zheng-Yu","family":"Yan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Xia","family":"Ai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi-Long","family":"Su","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin-Ying","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Hui","family":"Shan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng-Mei","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2015,12,21]]},"reference":[{"key":"S1431927615015548_ref27","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2011.09.016"},{"key":"S1431927615015548_ref28","doi-asserted-by":"publisher","DOI":"10.1007\/s10854-014-1838-x"},{"key":"S1431927615015548_ref2","doi-asserted-by":"publisher","DOI":"10.2116\/analsci.18.737"},{"key":"S1431927615015548_ref25","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-003-1027-6"},{"key":"S1431927615015548_ref17","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90268-U"},{"key":"S1431927615015548_ref21","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1008368107"},{"key":"S1431927615015548_ref13","doi-asserted-by":"publisher","DOI":"10.1021\/ac301918t"},{"key":"S1431927615015548_ref9","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3161"},{"key":"S1431927615015548_ref14","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2259"},{"key":"S1431927615015548_ref3","doi-asserted-by":"publisher","DOI":"10.1080\/000187300405228"},{"key":"S1431927615015548_ref23","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-012-9839-3"},{"key":"S1431927615015548_ref7","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.200801492"},{"key":"S1431927615015548_ref200","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.5.1664-1672.1999"},{"key":"S1431927615015548_ref26","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01455-13"},{"key":"S1431927615015548_ref4","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2007.460"},{"key":"S1431927615015548_ref32","doi-asserted-by":"publisher","DOI":"10.1186\/1556-276X-7-480"},{"key":"S1431927615015548_ref10","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01962-10"},{"key":"S1431927615015548_ref38","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0092486"},{"key":"S1431927615015548_ref15","doi-asserted-by":"publisher","DOI":"10.1128\/JB.05534-11"},{"key":"S1431927615015548_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2012.10.030"},{"key":"S1431927615015548_ref5","doi-asserted-by":"publisher","DOI":"10.1038\/509S6a"},{"key":"S1431927615015548_ref20","doi-asserted-by":"publisher","DOI":"10.1021\/nn305346a"},{"key":"S1431927615015548_ref36","doi-asserted-by":"publisher","DOI":"10.1088\/2053-1591\/1\/1\/015401"},{"key":"S1431927615015548_ref35","first-page":"119","article-title":"Effect 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