{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T22:01:42Z","timestamp":1791237702927,"version":"4.1.0"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2019,2,4]],"date-time":"2019-02-04T00:00:00Z","timestamp":1549238400000},"content-version":"unspecified","delay-in-days":3,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microsc Microanal"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Through a correlative analytical approach encompassing backscattered electron scanning electron microscopy (BSE-SEM), energy dispersive X-ray spectroscopy (EDX), and micro-Raman spectroscopy, the composition of the mineralized biofilm around a dental implant, retrieved due to peri-implantitis, was investigated. The mineralized biofilm contains two morphologically distinct regions: (i) bacteria-containing zones (Bact+), characterized by aggregations of unmineralized and mineralized bacteria, and intermicrobial mineralization, and (ii) bacteria-free zones (Bact\u2212), comprised mainly of randomly oriented mineral platelets. Intramicrobial mineralization, within Bact+, appears as smooth, solid mineral deposits resembling the morphologies of dental plaque bacteria. Bact\u2212 is associated with micrometer-sized Mg-rich mineral nodules. The Ca\/P ratio of Bact+ is higher than Bact\u2212. The inorganic phase of Bact+ is carbonated apatite (CHAp), while that of Bact\u2212 is predominantly octacalcium phosphate (OCP) and whitlockite (WL) inclusions. Compared with native bone, the inorganic phase of Bact+ (i.e., CHAp) exhibits higher mineral crystallinity, lower carbonate content, and lower Ca\/P, C\/Ca, Mg\/Ca, and Mg\/P ratios. The various CaPs found within the mineralized dental biofilm (CHAp, OCP, and WL) are related to the local presence\/absence of bacteria. In combination with BSE-SEM and EDX, micro-Raman spectroscopy is a valuable analytical tool for nondestructive investigation of mineralized dental biofilm composition and development.<\/jats:p>","DOI":"10.1017\/s1431927618015659","type":"journal-article","created":{"date-parts":[[2019,2,4]],"date-time":"2019-02-04T07:01:07Z","timestamp":1549263667000},"page":"129-134","source":"Crossref","is-referenced-by-count":26,"title":["Micro-Raman Spectroscopy Reveals the Presence of Octacalcium Phosphate and Whitlockite in Association with Bacteria-Free Zones Within the Mineralized Dental Biofilm"],"prefix":"10.1093","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9876-0467","authenticated-orcid":false,"given":"Furqan A.","family":"Shah","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,2,4]]},"reference":[{"key":"S1431927618015659_ref25","doi-asserted-by":"publisher","DOI":"10.1039\/C5TB02247A"},{"key":"S1431927618015659_ref26","doi-asserted-by":"publisher","DOI":"10.1177\/00220345930720121401"},{"key":"S1431927618015659_ref28","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-103-25479"},{"key":"S1431927618015659_ref14","doi-asserted-by":"publisher","DOI":"10.1177\/00220345830620021901"},{"key":"S1431927618015659_ref18","doi-asserted-by":"publisher","DOI":"10.1007\/BF02547382"},{"key":"S1431927618015659_ref7","doi-asserted-by":"publisher","DOI":"10.1007\/BF02556696"},{"key":"S1431927618015659_ref16","doi-asserted-by":"publisher","DOI":"10.1007\/BF02554841"},{"key":"S1431927618015659_ref17","doi-asserted-by":"publisher","DOI":"10.1177\/08959374960100021801"},{"key":"S1431927618015659_ref3","doi-asserted-by":"publisher","DOI":"10.1038\/srep32671"},{"key":"S1431927618015659_ref2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0501.2008.01589.x"},{"key":"S1431927618015659_ref27","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2005.01.008"},{"key":"S1431927618015659_ref23","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.7b02888"},{"key":"S1431927618015659_ref1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12151"},{"key":"S1431927618015659_ref8","doi-asserted-by":"publisher","DOI":"10.1021\/cm9604266"},{"key":"S1431927618015659_ref4","doi-asserted-by":"publisher","DOI":"10.1117\/1.3505010"},{"key":"S1431927618015659_ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.mattod.2013.05.003"},{"key":"S1431927618015659_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2016.08.041"},{"key":"S1431927618015659_ref6","doi-asserted-by":"publisher","DOI":"10.1002\/jrs.5175"},{"key":"S1431927618015659_ref9","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.94"},{"key":"S1431927618015659_ref10","doi-asserted-by":"publisher","DOI":"10.1126\/science.131.3394.156"},{"key":"S1431927618015659_ref11","doi-asserted-by":"publisher","DOI":"10.1038\/bdjopen.2015.3"},{"key":"S1431927618015659_ref12","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-017-0303-0"},{"key":"S1431927618015659_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4165(00)00157-4"},{"key":"S1431927618015659_ref15","first-page":"73","article-title":"Magnesium-containing crystals in human dental calculus","volume":"37","author":"Kodaka","year":"1988","journal-title":"J Electron Microsc (Tokyo)"},{"key":"S1431927618015659_ref19","doi-asserted-by":"publisher","DOI":"10.1080\/05704928.2014.986734"},{"key":"S1431927618015659_ref20","doi-asserted-by":"publisher","DOI":"10.1126\/science.135.3502.439"},{"key":"S1431927618015659_ref21","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1963.tb13458.x"},{"key":"S1431927618015659_ref22","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1969.40.11.643"},{"key":"S1431927618015659_ref29","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b01271"}],"container-title":["Microscopy and Microanalysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S1431927618015659","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T21:31:07Z","timestamp":1676410267000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/mam\/article\/25\/1\/129\/6887495"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["S1431927618015659"],"URL":"https:\/\/doi.org\/10.1017\/s1431927618015659","relation":{},"ISSN":["1431-9276","1435-8115"],"issn-type":[{"value":"1431-9276","type":"print"},{"value":"1435-8115","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2]]}}}