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The main prevention and control of the infection are meat inspection by the magnetic stirrer method for the detection of <jats:italic>Trichinella<\/jats:italic> larvae in muscle samples. The treatment can be effective if the parasite is discovered early in the intestinal phase. Once the <jats:italic>Trichinella<\/jats:italic> larva has reached the muscle tissue, the parasite remains therein and there is no treatment for this life cycle stage. The <jats:italic>Trichinella<\/jats:italic> species is dioecious with separate male and female individuals. The developed staining technique that uses confocal laser scanning microscopy (CLSM) displays sufficient results for <jats:italic>Trichinella<\/jats:italic> larvae examination and this protocol is applicable to study the internal and external structures and for the sex determination of <jats:italic>T. britovi<\/jats:italic> and <jats:italic>T. spiralis<\/jats:italic> larvae samples. In the present study, a luminescent derivative was synthesized and used for staining of <jats:italic>T. spiralis<\/jats:italic> and <jats:italic>T. britovi<\/jats:italic> larvae samples for the examination by CLSM. Various fixatives, such as AFA, 70% ethanol, and Bouin's and Carnoy's solutions were tested for sample preparation. The synthesized luminescent compound demonstrates best visualization results for samples fixed in Bouin's fixative.<\/jats:p>","DOI":"10.1017\/s1431927619015046","type":"journal-article","created":{"date-parts":[[2019,10,28]],"date-time":"2019-10-28T07:05:59Z","timestamp":1572246359000},"page":"1491-1497","source":"Crossref","is-referenced-by-count":15,"title":["A Simple and Rapid Staining Technique for Sex Determination of <i>Trichinella<\/i> Larvae Parasites by Confocal Laser Scanning 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