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Male C57BL\/6 mice were divided into groups: Control\u2014fed with standard chow, BET\u2014standard chow supplemented with betaine (1.5% w\/v drinking water), MCD\u2014fed with MCD diet, and MCD + BET\u2014MCD diet with betaine supplementation for 6 weeks. Liver samples were taken for pathohistology and transmission electron microscopy. The MCD diet-induced steatosis, inflammation, and balloon-altered hepatocytes were alleviated by betaine. MCD diet induced an increase in mitochondrial size versus the control group (<jats:italic>p<\/jats:italic> &lt; 0.01), which was decreased in the betaine-treated group. In the MCD diet-fed group, the total mitochondrial count decreased versus the control group (<jats:italic>p<\/jats:italic> &lt; 0.01), while it increased in the MCD + BET group versus MCD (<jats:italic>p<\/jats:italic> &lt; 0.01). Electron microscopy showed an increase in the number of autophagosomes in the MCD and MCD + BET group versus control, and a significant difference in autophagosomes number was detected in the MCD + BET group by comparison with the MCD diet-treated group (<jats:italic>p<\/jats:italic> &lt; 0.05). Betaine decreases the number of enlarged mitochondria, alleviates steatosis, and increases the number of autophagosomes in the liver of mice with NAFLD.<\/jats:p>","DOI":"10.1017\/s1431927620024265","type":"journal-article","created":{"date-parts":[[2020,8,12]],"date-time":"2020-08-12T07:46:23Z","timestamp":1597218383000},"page":"997-1006","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":20,"title":["Effect of Betaine Supplementation on Liver Tissue and Ultrastructural Changes in Methionine\u2013Choline-Deficient Diet-Induced 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