{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T22:11:41Z","timestamp":1759961501255,"version":"3.40.5"},"reference-count":17,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2021,6,22]],"date-time":"2021-06-22T00:00:00Z","timestamp":1624320000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Microsc Microanal"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:p>The ductus deferens is a fundamental part of the male genital tract and the continuation of the epididymal duct. As a male secondary sex organ, the ductus deferens plays a crucial role in the nourishment, storage, and maturation of spermatozoa. Some studies have provided information about the ductus deferens structure in reptiles; however, the full description of the ductus deferens remains to be clarified. The current study aimed to describe the Nile monitor lizard (<jats:italic>Varanus niloticus)<\/jats:italic>ductus deferens from histological, histochemical, and ultrastructural perspectives. The results revealed that the ductus deferens is formed histologically from two main cell types: principal and basal. The principal cells were tall and filled with periodic acid Schiff (+)\/alcian blue (\u2212) cytoplasmic granules. The basal cells were found just above the basement membrane. By transmission electron microscopy, the principal cells exhibited typical protein-secreting cell features. Additionally, some intraepithelial cells, such as halo cells, undifferentiated mesenchymal cells, and agranular leukocytes, were identified. This study presents the first detailed description of the<jats:italic>Varanus niloticus<\/jats:italic>ductus deferens. Further immunohistochemical studies are required to explore the function(s) of the cellular components.<\/jats:p>","DOI":"10.1017\/s1431927621012046","type":"journal-article","created":{"date-parts":[[2021,6,22]],"date-time":"2021-06-22T06:14:15Z","timestamp":1624342455000},"page":"935-940","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":2,"title":["Histological, Histochemical, and Ultrastructural Approach to the Ductus Deferens in Male Nile Monitor Lizard (<i>Varanus niloticus<\/i>)"],"prefix":"10.1093","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2882-6280","authenticated-orcid":false,"given":"Mahmoud","family":"Awad","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammed","family":"Alshehri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed Saad A.","family":"Hassaneen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,6,22]]},"reference":[{"key":"S1431927621012046_ref5","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1090690105"},{"key":"S1431927621012046_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/S0940-9602(04)80009-8"},{"volume-title":"Bancroft's Theory and Practice of Histological Techniques","year":"2013","author":"Bancroft","key":"S1431927621012046_ref2"},{"key":"S1431927621012046_ref10","first-page":"127","article-title":"Is there any interspecific competition between dwarf crocodiles (Osteolaemus tetraspis) and Nile monitors (Varanus niloticus ornatus) in the swamps of Central Africa? A study from south-eastern","volume":"247","author":"Luiselli","year":"1999","journal-title":"Nigeria J Zool"},{"volume-title":"Goannas: The Biology of Varanid Lizards (Australian Natural History Series)","year":"1999","author":"King","key":"S1431927621012046_ref9"},{"volume-title":"Diagnostic Electron Microscopy","year":"1978","author":"Burns","key":"S1431927621012046_ref4"},{"key":"S1431927621012046_ref15","doi-asserted-by":"publisher","DOI":"10.1046\/j.1524-4725.2000.09220.x"},{"key":"S1431927621012046_ref1","first-page":"1","article-title":"Histochemical and ultrastructural investigations on the renal parenchyma of the Egyptian Nile Monitor Lizard (Varanus niloticus)","author":"Awad","year":"2019","journal-title":"Acta Zool"},{"key":"S1431927621012046_ref11","doi-asserted-by":"crossref","first-page":"72","DOI":"10.21157\/j.ked.hewan.v10i1.3375","article-title":"Micromorphological structure of primary reproductive organ of male water monitor lizard (Varanus salvator bivittatus)","volume":"10","author":"Mahfud","year":"2016","journal-title":"J Ked Hewan"},{"key":"S1431927621012046_ref14","first-page":"79","article-title":"Goanna metabolism: Is it different to other lizards, and if so what are the ecological consequences?","volume":"11","author":"Thompson","year":"1999","journal-title":"Mertensiella"},{"key":"S1431927621012046_ref12","doi-asserted-by":"publisher","DOI":"10.7589\/2013-01-002"},{"key":"S1431927621012046_ref3","doi-asserted-by":"publisher","DOI":"10.1670\/0022-1511(2002)036[0116:DOJVNS]2.0.CO;2"},{"key":"S1431927621012046_ref16","doi-asserted-by":"publisher","DOI":"10.1590\/1676-06032014006413"},{"key":"S1431927621012046_ref13","first-page":"407","article-title":"Alcian blue technics for the histochemical study of acidic carbohydrates","volume":"4","author":"Mowry","year":"1956","journal-title":"J Histochem Cytochem"},{"key":"S1431927621012046_ref6","first-page":"262","article-title":"Preliminary data on size, growth and avoidance of the Nile monitor lizard (Varanus niloticus) in the region of Lake Chad (in French: Donne\u00b4es pre\u00b4liminaires sur la taille, la croissance et la longe \u00b4vite\u00b4du varan du Nil (Varanus niloticus) dans la re\u00b4gion du Lac Tchad)","volume":"72","author":"de Buffrenil","year":"1994","journal-title":"Can J Zool"},{"key":"S1431927621012046_ref17","unstructured":"Wyneken, J & Mader, D (2002). The reproductive system of reptiles\u2014Anatomy, physiology and clinical perspectives. In 2002 Proceedings. Association of Reptilian and Amphibian Veterinarians, p. 187."},{"key":"S1431927621012046_ref8","first-page":"777","article-title":"On the rapid conversion of hematoxylin into haematein in staining reactions","volume":"3","author":"Harris","year":"1900","journal-title":"J Appl Microsc Lab Methods"}],"container-title":["Microscopy and Microanalysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S1431927621012046","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T06:45:28Z","timestamp":1699166728000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/mam\/article\/27\/4\/935\/6888013"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,22]]},"references-count":17,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["S1431927621012046"],"URL":"https:\/\/doi.org\/10.1017\/s1431927621012046","relation":{},"ISSN":["1431-9276","1435-8115"],"issn-type":[{"type":"print","value":"1431-9276"},{"type":"electronic","value":"1435-8115"}],"subject":[],"published":{"date-parts":[[2021,6,22]]},"assertion":[{"value":"Copyright \u00a9 The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}