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The constipation and treatment models are generated using adult male C57BL\/6N mice. We perform light microscopy and transmission electron microscopy (TEM) to detect a Muc2-rich inner mucus layer attached to mice colon under different conditions. In addition, 16S rDNA sequencing is performed to examine the intestinal flora. According to TEM images, we demonstrate that Rhubarb can promote mucin secretion and find direct evidence of dendritic structure-linked mucus structures with its assembly into a lamellar network in a pore size distribution in the isolated colon section. Moreover, the diversity of intestinal flora has noticeable changes in constipated mice. The present study characterizes a dendritic structure and persistent cross-links have significant changes accompanied by the alteration of intestinal flora in feces in models of constipation and pretreatment with Rhubarb extract.<\/jats:p>","DOI":"10.1017\/s1431927622000836","type":"journal-article","created":{"date-parts":[[2022,5,30]],"date-time":"2022-05-30T04:58:35Z","timestamp":1653886715000},"page":"1720-1733","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":6,"title":["An Unexpected Alteration Colonic Mucus Appearance in the Constipation Model via an Intestinal Microenvironment"],"prefix":"10.1093","volume":"28","author":[{"given":"Han","family":"Gao","sequence":"first","affiliation":[{"name":"Capital Medical University Department of Physiology and Pathophysiology, School of Basic Medical Science, , Beijing 100069, 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