{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T17:02:23Z","timestamp":1740157343950,"version":"3.37.3"},"reference-count":32,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[2018,7,5]],"date-time":"2018-07-05T00:00:00Z","timestamp":1530748800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"},{"start":{"date-parts":[[2018,7,5]],"date-time":"2018-07-05T00:00:00Z","timestamp":1530748800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["J Dev Orig Health Dis"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Impaired \u03b2-cell development and insulin secretion are characteristic of intrauterine growth-restricted (IUGR) fetuses. In normally grown late gestation fetal sheep pancreatic \u03b2-cell numbers and insulin secretion are increased by 7\u201310 days of pulsatile hyperglycemia (PHG). Our objective was to determine if IUGR fetal sheep \u03b2-cell numbers and insulin secretion could also be increased by PHG or if IUGR fetal \u03b2-cells do not have the capacity to respond to PHG. Following chronic placental insufficiency producing IUGR in twin gestation pregnancies (<jats:italic>n<\/jats:italic>=7), fetuses were administered a PHG infusion, consisting of 60 min, high rate, pulsed infusions of dextrose three times a day with an additional continuous, low-rate infusion of dextrose to prevent a decrease in glucose concentrations between the pulses or a control saline infusion. PHG fetuses were compared with their twin IUGR fetus, which received a saline infusion for 7 days. The pulsed glucose infusion increased fetal arterial glucose concentrations an average of 83% during the infusion. Following the 7-day infusion, a square-wave fetal hyperglycemic clamp was performed in both groups to measure insulin secretion. The rate of increase in fetal insulin concentrations during the first 20 min of a square-wave hyperglycemic clamp was 44% faster in the PHG fetuses compared with saline fetuses (<jats:italic>P<\/jats:italic>&lt;0.05). There were no differences in islet size, the insulin<jats:sup>+<\/jats:sup> area of the pancreas and of the islets, and \u03b2-cell mass between groups (<jats:italic>P<\/jats:italic>&gt;0.23). Chronic PHG increases early phase insulin secretion in response to acute hyperglycemia, indicating that IUGR fetal \u03b2-cells are functionally responsive to chronic PHG.<\/jats:p>","DOI":"10.1017\/s2040174418000417","type":"journal-article","created":{"date-parts":[[2018,7,5]],"date-time":"2018-07-05T04:50:38Z","timestamp":1530766238000},"page":"492-499","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":4,"title":["Pulsatile hyperglycemia increases insulin secretion but not pancreatic \u03b2-cell mass in intrauterine growth-restricted fetal sheep"],"prefix":"10.1017","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8895-9299","authenticated-orcid":false,"given":"B. H.","family":"Boehmer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. D.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. R.","family":"Wesolowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"W. W.","family":"Hay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. J.","family":"Rozance","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2018,7,5]]},"reference":[{"key":"S2040174418000417_ref17","doi-asserted-by":"crossref","unstructured":"Benjamin JS , Culpepper CB , Brown LD , et al. Chronic anemic hypoxemia attenuates glucose-stimulated insulin secretion in fetal sheep. 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