{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T20:14:03Z","timestamp":1786479243776,"version":"build-2736575974"},"reference-count":50,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2019,11,22]],"date-time":"2019-11-22T00:00:00Z","timestamp":1574380800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Dev Orig Health Dis"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Placental lipids transfer is essential for optimal fetal development, and alterations of these mechanisms could lead to a higher risk of adverse birth outcomes. Low-density lipoprotein receptor (<jats:italic>LDLR<\/jats:italic>), LDL receptor-related protein 1 (<jats:italic>LRP1<\/jats:italic>), and scavenger receptor class B type 1 (<jats:italic>SCARB1<\/jats:italic>) genes are encoding lipoprotein receptors expressed in the placenta where they participate in cholesterol exchange from maternal to fetal circulation. The aim of this study was thus to investigate the association between maternal lipid changes occurring in pregnancy, placental DNA methylation (DNAm) variations at <jats:italic>LDLR, LRP1<\/jats:italic>, and <jats:italic>SCARB1<\/jats:italic> gene loci, and newborn\u2019s anthropometric profile at birth. Sixty-nine normoglycemic women were followed from the first trimester of pregnancy until delivery. Placental DNAm was quantified at 43 Cytosine-phosphate-Guanines (CpGs) at <jats:italic>LDLR, LRP1<\/jats:italic>, and <jats:italic>SCARB1<\/jats:italic> gene loci using pyrosequencing: 4 CpGs were retained for further analysis. Maternal clinical data were collected at each trimester of pregnancy. Newborns\u2019 data were collected from medical records. Statistical models included minimally newborn sex and gestational and maternal age. Maternal total cholesterol changes during pregnancy (\u0394T3-T1) were correlated with DNAm variations at <jats:italic>LDLR<\/jats:italic> (<jats:italic>r<\/jats:italic> = \u22120.32, <jats:italic>p<\/jats:italic> = 0.01) and <jats:italic>LRP1<\/jats:italic> (<jats:italic>r<\/jats:italic> = 0.34, <jats:italic>p<\/jats:italic> = 0.007). DNAm at these loci was also correlated with newborns\u2019 cord blood triglyceride and leptin levels. Mediation analysis supports a causal relationship between maternal cholesterol changes, DNAm levels at <jats:italic>LRP1<\/jats:italic> locus, and cord blood leptin concentration (<jats:italic>p<\/jats:italic><jats:sub>mediation<\/jats:sub> = 0.02). These results suggest that <jats:italic>LRP1<\/jats:italic> DNAm link maternal blood cholesterol changes in pregnancy and offspring adiposity at birth, which provide support for a better follow-up of blood lipids in pregnancy.<\/jats:p>","DOI":"10.1017\/s204017441900076x","type":"journal-article","created":{"date-parts":[[2019,11,22]],"date-time":"2019-11-22T05:56:56Z","timestamp":1574402216000},"page":"369-378","source":"Crossref","is-referenced-by-count":14,"title":["DNA methylation at <i>LRP1<\/i> gene locus mediates the association between maternal total cholesterol changes in pregnancy and cord blood leptin 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