{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T02:19:26Z","timestamp":1788315566622,"version":"build-2803163510"},"reference-count":64,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T00:00:00Z","timestamp":1747785600000},"content-version":"unspecified","delay-in-days":140,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["J Dev Orig Health Dis"],"published-print":{"date-parts":[[2025]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t  <jats:p>Pre-pregnancy obesity (ppOB) is linked to pregnancy complications and abnormal fetal growth through placental mechanisms, and long non-coding RNAs (lncRNAs) may play an epigenetic role in these processes. We investigated overall and sex-specific associations of pre-pregnancy body mass index (ppBMI), ppOB, and birthweight with placental lncRNA transcripts in two birth cohorts. Study participants were mother-child dyads recruited to the CANDLE (Memphis, TN)(<jats:italic>n<\/jats:italic> = 725) and GAPPS (Seattle and Yakima, WA)(<jats:italic>n<\/jats:italic> = 159) cohorts. Maternal ppBMI was assessed at enrollment using interviewer-administered questionnaires. LncRNAs (1,077 and 1,033 for CANDLE and GAPPS, respectively) were sequenced from placental samples collected at birth. Placental lncRNA was regressed on ppBMI, ppOB (ppBMI \u226530kg\/m<jats:sup>2<\/jats:sup>), or continuous birthweight in cohort-specific weighted linear models controlling for <jats:italic>a priori<\/jats:italic>-specified confounders and experimental variables. Potential effect modification by infant-sex was examined in sex-stratified analyses and models including BMI-infant-sex interaction terms. No lncRNA transcripts were significantly associated with ppBMI, ppOB, or birthweight in primary models. Among male infants in CANDLE, expression of three lncRNA transcripts (<jats:italic>ERVH48-1<\/jats:italic>, <jats:italic>AC139099.1<\/jats:italic>, <jats:italic>CEBPA-DT<\/jats:italic>) was associated with ppBMI and one transcript (<jats:italic>AC104083.1<\/jats:italic>) with birthweight. In GAPPS, ppBMI was associated with two lncRNA transcripts (<jats:italic>AP000879.1<\/jats:italic> and <jats:italic>AL365203.2<\/jats:italic>) among males, and birthweight was associated with 17 lncRNA transcripts (including <jats:italic>LINC02709<\/jats:italic>, <jats:italic>KANSL1-AS1, DANCR, EPB41L4A-AS1<\/jats:italic>, and <jats:italic>GABPB1-AS1<\/jats:italic>) among females. No BMI-infant-sex interactions were observed. Though many of these potential associations are for uncharacterized transcripts, several identified lncRNAs (e.g., <jats:italic>ERVH48-1<\/jats:italic> and <jats:italic>CEBPA-DT<\/jats:italic>) have been linked to pathways controlling cancer or placental growth, trophoblast differentiation, and gene expression. These associations warrant validation in future studies.<\/jats:p>","DOI":"10.1017\/s204017442500011x","type":"journal-article","created":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T03:44:35Z","timestamp":1747799075000},"update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":2,"title":["Associations of placental lncRNA expression with maternal pre-pregnancy BMI and infant birthweight in two birth cohorts"],"prefix":"10.1017","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4234-0030","authenticated-orcid":false,"given":"Michael R.","family":"Hussey","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"MacDonald","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theo K.","family":"Bammler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fasil","family":"Tekola-Ayele","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen F.","family":"Kerr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison G.","family":"Paquette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4566-150X","authenticated-orcid":false,"given":"Carmen J.","family":"Marsit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaja Z.","family":"LeWinn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine J.","family":"Karr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheela","family":"Sathyanarayana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel A.","family":"Enquobahrie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2025,5,21]]},"reference":[{"key":"S204017442500011X_ref41","doi-asserted-by":"publisher","DOI":"10.1017\/S2040174417000927"},{"key":"S204017442500011X_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.siny.2009.09.006"},{"key":"S204017442500011X_ref40","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2016.12.008"},{"key":"S204017442500011X_ref55","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2021.740134"},{"key":"S204017442500011X_ref56","doi-asserted-by":"publisher","DOI":"10.21037\/tp-22-253"},{"key":"S204017442500011X_ref3","doi-asserted-by":"publisher","DOI":"10.2174\/1570161118666200513115316"},{"key":"S204017442500011X_ref35","doi-asserted-by":"publisher","DOI":"10.1159\/000495086"},{"key":"S204017442500011X_ref62","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033294"},{"key":"S204017442500011X_ref37","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-55933-x"},{"key":"S204017442500011X_ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.03.050"},{"key":"S204017442500011X_ref58","doi-asserted-by":"publisher","DOI":"10.1007\/s43032-021-00687-z"},{"key":"S204017442500011X_ref39","doi-asserted-by":"publisher","DOI":"10.1093\/eep\/dvaa003"},{"key":"S204017442500011X_ref51","doi-asserted-by":"publisher","DOI":"10.1126\/science.aah7111"},{"key":"S204017442500011X_ref17","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2022-064288"},{"key":"S204017442500011X_ref54","doi-asserted-by":"publisher","DOI":"10.1186\/s12865-021-00439-3"},{"key":"S204017442500011X_ref1","doi-asserted-by":"publisher","DOI":"10.1017\/S2040174410000280"},{"key":"S204017442500011X_ref61","doi-asserted-by":"publisher","DOI":"10.1038\/sj.clpt.6100376"},{"key":"S204017442500011X_ref10","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-020-00315-9"},{"key":"S204017442500011X_ref26","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2023.05.004"},{"key":"S204017442500011X_ref52","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2019.09.017"},{"key":"S204017442500011X_ref16","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-95051-1"},{"key":"S204017442500011X_ref13","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19071894"},{"key":"S204017442500011X_ref28","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts034"},{"key":"S204017442500011X_ref64","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2019.110622"},{"key":"S204017442500011X_ref33","first-page":"1","article-title":"Knockdown 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Published by Cambridge University Press in association with The International Society for Developmental Origins of Health and Disease (DOHaD)","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (https:\/\/creativecommons.org\/licenses\/by\/4.0\/), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}],"article-number":"e20"}}